LOCUS       sequence-331407-        6976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Arabinose-inducible expression of phzSM for conversion of 
            phenazine-1-carboxylic acid to pyocyanin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6976)
  TITLE     Bioelectronic sensors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSB4A3-PBAD-phzSM
FEATURES             Location/Qualifiers
     source          1..6976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      21..78
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(102..119)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(381..1331)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      1379..1601
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2093..2187)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2217..3008,3009..3077))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2840..2859
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3078..3182)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3247..3265
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3269..3312)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3315..3336
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             complement(3337..4215)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4242..4526
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4449..4468
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      6855..6926
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6942..6969
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    join(6976,1..20)
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 actagtagcg gccgctgcag tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt
       61 tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg
      121 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatctcgagg
      181 ttacattgtc gatctgttca tggtgaacag ctttgaatgc accaaaaact cgtaaaagct
      241 ctgatgtatc tatctttttt acaccgtttt catctgtgca tatggacagt tttccctttg
      301 atatgtaacg gtgaacagtt gttctacttt tgtttgttag tcttgatgct tcactgatag
      361 atacaagagc cataagaacc tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg
      421 gttcgttgtt tttgcgtgag ccatgagaac gaaccattga gatcatactt actttgcatg
      481 tcactcaaaa attttgcctc aaaactggtg agctgaattt ttgcagttaa agcatcgtgt
      541 agtgtttttc ttagtccgtt atgtaggtag gaatctgatg taatggttgt tggtattttg
      601 tcaccattca tttttatctg gttgttctca agttcggtta cgagatccat ttgtctatct
      661 agttcaactt ggaaaatcaa cgtatcagtc gggcggcctc gcttatcaac caccaatttc
      721 atattgctgt aagtgtttaa atctttactt attggtttca aaacccattg gttaagcctt
      781 ttaaactcat ggtagttatt ttcaagcatt aacatgaact taaattcatc aaggctaatc
      841 tctatatttg ccttgtgagt tttcttttgt gttagttctt ttaataacca ctcataaatc
      901 ctcatagagt atttgttttc aaaagactta acatgttcca gattatattt tatgaatttt
      961 tttaactgga aaagataagg caatatctct tcactaaaaa ctaattctaa tttttcgctt
     1021 gagaacttgg catagtttgt ccactggaaa atctcaaagc ctttaaccaa aggattcctg
     1081 atttccacag ttctcgtcat cagctctctg gttgctttag ctaatacacc ataagcattt
     1141 tccctactga tgttcatcat ctgagcgtat tggttataag tgaacgatac cgtccgttct
     1201 ttccttgtag ggttttcaat cgtggggttg agtagtgcca cacagcataa aattagcttg
     1261 gtttcatgct ccgttaagtc atagcgacta atcgctagtt catttgcttt gaaaacaact
     1321 aattcagaca tacatctcaa ttggtctagg tgattttaat cactatacca attgagatgg
     1381 gctagtcaat gataattaca tgtccttttc ctttgagttg tgggtatctg taaattctgc
     1441 tagacctttg ctggaaaact tgtaaattct gctagaccct ctgtaaattc cgctagacct
     1501 ttgtgtgttt tttttgttta tattcaagtg gttataattt atagaataaa gaaagaataa
     1561 aaaaagataa aaagaataga tcccagccct gtgtataact cactacttta gtcagttccg
     1621 cagtattaca aaaggatgtc gcaaacgctg tttgctcctc tacaaaacag accttaaaac
     1681 cctaaaggct taagtagcac cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc
     1741 tccgaccatc aggcacctga gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac
     1801 ggctctggca gtgaatgggg gtaaatggca ctacaggcgc cttttatgga ttcatgcaag
     1861 gaaactaccc ataatacaag aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg
     1921 tctgctatgt ggtgctatct gactttttgc tgttcagcag ttcctgccct ctgattttcc
     1981 agtctgacca cttcggatta tcccgtgaca ggtcattcag actggctaat gcacccagta
     2041 aggcagcggt atcatcaaca ggcttacccg tcttactgtc cctagtgctt ggattctcac
     2101 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
     2161 tcattactgg atctatcaac aggagtccaa gcgagctcgt aaacttggtc tgacagttac
     2221 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2281 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     2341 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     2401 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     2461 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     2521 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     2581 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     2641 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2701 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2761 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     2821 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     2881 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     2941 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3001 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3061 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3121 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3181 cgcacatttc ccatggtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     3241 tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct
     3301 aaggatgatt tctggaattc gcggccgctt ctagagttat gacaacttga cggctacatc
     3361 attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt
     3421 aaatacccgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat
     3481 aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca
     3541 gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa
     3601 gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat
     3661 gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc
     3721 ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg
     3781 cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg
     3841 cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt gacggccagt taagccattc
     3901 atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg
     3961 atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca
     4021 aacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat
     4081 ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat
     4141 cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt
     4201 ttgcgcttca gccatacttt tcatactccc gccattcaga gaagaaacca attgtccata
     4261 ttgcatcaga cattgccgtc actgcgtctt ttactggctc ttctcgctaa ccaaaccggt
     4321 aaccccgctt attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg
     4381 taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac
     4441 actttgctat gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt
     4501 atcgcaactc tctactgttt ctccattact agagccatcg attcgaacac tcgagaaaag
     4561 gaagcaccca tgagcgaacc catcgatatc ctcatcgccg gcgccggcat cggcggcctc
     4621 agttgcgccc tggccctgca ccaggccggc atcggcaagg tcacgctgct ggaaagcagc
     4681 agcgagatac gcccccttgg cgtcggcatc aatatccagc cggcggcggt cgaggccctt
     4741 gccgaactgg gcctcggccc ggcgctggcg gccaccgcca tccccaccca cgagctgcgc
     4801 tacatcgacc agagcggcgc cacggtatgg tccgagccgc gcggggtgga agccggcaac
     4861 gcctatccgc agtactcgat ccatcgcggc gaattgcaga tgatcctgct cgccgcggtg
     4921 cgcgagcgtc tcggccaaca ggcggtacgc accggtctcg gcgtggagcg catcgaagag
     4981 cgcgacggcc gcgtactgat cggcgcccgc gacggacacg gcaagcccct ggcgctcggt
     5041 gccgatgtgc tggtcggcgc cgacggcatc cattcggcgg tccgcgcgca cctgcatccc
     5101 gaccaggggc cgctgtccca cggtgggatc accatgtggc gcggcgtcac cgagttcgac
     5161 cgcttcctcg acggcaagac catgatcgtc gccaacgacg agcactggtc gcgcctggtc
     5221 gcctatccga tctcggcgcg ccacgcggcc gaaggcaagt cgctggtgaa ctgggtgtgc
     5281 atggtgccga gcgccgccgt cggccagctc gacaacgagg ccgactggaa ccgcaacgga
     5341 cgcctggaag acgtgttgcc gttcttcgcc gactgggacc tgggctggtt cgacatccgc
     5401 gacctgctga cccgcaacca gttgatcttg cagtacccga tggtcgaccg cgatccgctg
     5461 ccgcactggg gccggggacg catcacgctg ctcggcgacg ccgcccacct gatgtatccg
     5521 atgggcgcca acggcgcttc gcaggcgatc ctcgacggca tcgagctggc cgccgcgctg
     5581 gcgcgcaacg ccgacgtggc cgcagccctg cgcgaatacg aagaagcgcg gcggccgacc
     5641 gccaacaaga tcatcctggc caaccgcgaa cgggaaaaag aggaatgggc cgcggcttcg
     5701 cgaccgaaga ctgaaaagag cgcggcgctg gaagcgatca ccggcagcta ccgcaaccag
     5761 gtggaacggc cacgctaata ctagagtggg tacttctcgg gttacggaaa tgtgtaacga
     5821 gagagaataa aagatgaata attcgaatct tgctgctgcg cgtaatttga tacaagttgt
     5881 taccggggaa tggaagtccc gttgcgtcta cgtcgctacg cgcctcgggc tggccgatct
     5941 gatcgagagc gggatcgaca gcgacgagac gctggccgcc gcggtcggtt ccgatgccga
     6001 gcgcatccat cgactgatgc gcctgctggt ggccttcgag atcttccagg gcgatacccg
     6061 cgacggctac gctaataccc ccaccagcca cctgctgagg gatgtcgagg gctccttccg
     6121 cgacatggtg ctgttctacg gcgaggagtt ccacgccgcc tggacgcccg cctgcgaggc
     6181 gctgctcagc ggtaccccag gcttcgaact ggcgttcggc gaagacttct acagctacct
     6241 gaagcgctgc ccggatgccg gccggcgctt cctgctggcg atgaaggcga gcaacctggc
     6301 cttccacgag atccccaggc tcctggattt ccgcgggcgt agcttcgtcg acgtcggtgg
     6361 cggttccggc gaattgacca aggccatcct gcaagccgag cccagcgccc ggggcgtgat
     6421 gctcgatcgc gagggttccc tcggcgtggc ccgcgacaac ctctccagcc tgttggcagg
     6481 ggagcgcgtc agcctggtgg gcggcgacat gctgcaagag gtgccgtcca acggcgatat
     6541 ctacctgctg tcgcggatca tcggcgatct ggacgaagcc gccagcctga ggttgctcgg
     6601 caattgccgc gaggcgatgg ccggcgacgg ccgggtggtg gtgatcgagc ggaccatctc
     6661 ggccagcgag ccgtcgccga tgtcggtgct ctgggacgtg cacctgttca tggcctgcgc
     6721 tggccgccac cgcaccaccg aggaggtggt cgacctgctc gggcgcggcg gcttcgcggt
     6781 ggagcggatc gtcgacctgc cgatggaaac ccgcatgatc gtcgctgcca gggcctaata
     6841 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     6901 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     6961 ctttctgcgt ttatat
//
