LOCUS       sequence-33141-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Tagwerker C, Zhang H, Wang X, Larsen LS, Lathrop RH, Hatfield GW, 
            Auer B, Huang L, Kaiser P
  TITLE     HB tag modules for PCR-based gene tagging and tandem affinity 
            purification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2006 Jun . 23(8):623-32.
  PUBMED    16823883
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Jun . 23(8):623-32."
COMMENT     Sequence Label: pFA6a-TRP1-PGAL1-HBH
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(191..295)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     363..381
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(419..441)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     541..560
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        641..659
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     641..658
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     843..867
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
       61 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
      121 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
      181 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
      241 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
      301 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
      361 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
      421 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
      481 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
      541 cggcatcaga gcagattgta ctgagagtgc accatatgga catattgtcg ttagaacgcg
      601 gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga cactatagaa
      661 cgcggccgcc agctgaagct tcgtacgctg caggtcgacg gatctatatt accctgttat
      721 ccctagcgga tctgccggta gaggtgtggt caataagagc gacctcatac tatacctgag
      781 aaagcaacct gacctacagg aaagagttac tcaagaataa gaattttcgt tttaaaacct
      841 aagagtcact ttaaaatttg tatacactta ttttttttat aacttattta ataataaaaa
//
