LOCUS       sequence-331410-        2768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and mEGFP-linker sequence for N-terminus tagging of 
            human PCNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2768)
  TITLE     Allen Institute for Cell Science Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-116:PCNA-mEGFP
FEATURES             Location/Qualifiers
     source          1..2768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      992..1001
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(998..1000,1001..1003,1004..1714)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1043..1064)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1304..1323)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1651..1672
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttaaatgtgt acgctctttg actcagctgt attacttcaa ggagttgata tcaccaaaat
       61 tgcctaagtg ctcaaaggtg tttgtagtta aacaacagga gattgataaa ttatgttata
      121 tacatgtgat gctatgtttt aaagaggtac tgatatgata aaagatgtac gtggcataaa
      181 attaaatgta ctttattaag tacttttcca agtgtttacg gaatgagtgc atttttgaaa
      241 aaaaaaaagt gtattcgaac ttttaaaaaa gctttaaaag ctttatacaa tgaacgattg
      301 agtgattata agagctggcg ggggaatgtt aagaggatga tagggagcta agtttaacag
      361 aacaattcac ctctttatct tgtgacacct acgagcgcat caattctgta attgaaaaat
      421 aaagtgcata tttgcagcag ctgtactctc ttcaggctgc aaggaggctt ttcctcccgg
      481 taggcttgat ttgcatttca ctttcacttt cgtggctgga aactttctac ccacgtagtg
      541 ggaggctgag gagccaccat aaagctgggg cttgacgagc cgggaccggg acccgatctc
      601 cacatatgcc cggacttgtt ctgcggccgg gttcaggagt caaagaggcg gggagacctg
      661 cgcgacgctg ccccgccctg cgcccgcttc ctccaatgta tgctctaggg ggcgggcctc
      721 gcggggagca tggacacgat tggccctaaa gtcttccccg caaggccgtg ggctggacag
      781 cgtggtgacg tcgcaacgcg gcgcagggtg agagcgcgcg cttgcggacg cggcggcatt
      841 aaacggttgc aggcgtagca gagtggtcgt tgtctttcta ggtctcagcc ggtcgtcgcg
      901 acgttcgccc gctcgctctg aggctcctga agccgaaacc agctagactt tcctccttcc
      961 cgcctgcctg tagcggcgtt gttgccactc cgccaccatg gtgagcaagg gcgaggagct
     1021 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1081 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1141 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     1201 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1261 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1321 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1381 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1441 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1501 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1561 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
     1621 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1681 cgggatcact ctcggcatgg acgagctgta caagggcgaa ggacagggcc agggacaggg
     1741 tccaggaaga gggtacgcct atagatcttt cgaggcgcgc ctcgtgcagg gctccatcct
     1801 caagaaggtg ttggaggcac tcaaggacct catcaacgag gcctgctggg atattagctc
     1861 cagcggtgta aacctgcaga gcatggactc gtcccacgtc tctttggtgc agctcaccct
     1921 gcggtctgag ggcttcgaca cctaccgctg cgaccgcaac ctggccatgg gcgtgaacct
     1981 caccaggtga gcctcgcggc cccgggaagc cggccccggc ccgcctgcac ctccggtgct
     2041 tggcgggagc gctttcgagc ctagccctca ttggctggcg tgggccatcc gcgccttctc
     2101 attggcctgc cacgcagtgg ggtggggccc agctgagcgc gcggttcgga aaagcccgcg
     2161 ctggctgctg cgcgaacctg ctttttcgcg ccaaagtcac aaagcgggtg gtggcgggaa
     2221 aatcaagggt ttttccgcag tgccaggaac actgttccag ggctctttgc tcactaaatc
     2281 ctgttggcct tgaatggacg ctttagctgt ggctttcttg tttctgagac ggtctcggtg
     2341 tgttgcccgg gctggtctcc aacttctggg ctcaagcgat cctcccggct cagtcgcggt
     2401 tgactttaaa tgctttataa tgcccttgcg agaaatgtgg cagcctgtca tcctacttag
     2461 tggcaggaga ttgtttctat ccagaaggga cactgctggt ggtattttag tataaatact
     2521 gccagatgcg tcccaaaacg tctgcattaa taatggcatc ctccagcagt ccgcttaccc
     2581 tccaccagtt ctgagacggc ctgatgggtg agagtggtaa ccccttctaa ccgcgttcga
     2641 aatacagccc ttcagcagac ggcgttgatt ttaaagcatg tgtctcctgt cttctagtat
     2701 gtccaaaata ctaaaatgcg ccggcaatga agatatcatt acactaaggg ccgaagataa
     2761 cgcggata
//
