LOCUS       sequence-33142-e         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Tagwerker C, Zhang H, Wang X, Larsen LS, Lathrop RH, Hatfield GW, 
            Auer B, Huang L, Kaiser P
  TITLE     HB tag modules for PCR-based gene tagging and tandem affinity 
            purification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2006 Jun . 23(8):623-32.
  PUBMED    16823883
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Jun . 23(8):623-32."
COMMENT     Sequence Label: pFA6a-TRP1-PGAL1-HBH
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(54..241)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     162..186
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(268..285)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(514..531)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(584..607)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 cgtacgctgc aggtcgacgg atctatatta ccctgttatc cctagcggat ctgccggtag
       61 aggtgtggtc aataagagcg acctcatact atacctgaga aagcaacctg acctacagga
      121 aagagttact caagaataag aattttcgtt ttaaaaccta agagtcactt taaaatttgt
      181 atacacttat tttttttata acttatttaa taataaaaat cataaatcat aagaaattcg
      241 cttatttaga agtggcgcgc cggctccatg atggtggtga tgatgaacgc cgatcttgat
      301 tagaccttga ccaccttgaa cagcatctct ttccttaact aacacctttt caacttttcc
      361 gtcagtagga gcgttaattt ctgtctccat tttcatagcc tccagcacca gaactgtttg
      421 accagcttta acagtgtcac cttcttttac cagtatctta gaaacggtac cagcaagagg
      481 ggcagggatt tcaccttcac cggcctttcc agcatgatgg tggtgatgat gtgaacccct
      541 catgttaatt aaagacattt tgagatccgg gttttttctc cttgacgtta aagtatagag
      601 gtatattaac aattttttgt tgatactttt attacatttg aataagaagt aatacaaacc
      661 gaaaatgttg aaagtattag ttaaagtggt tatgcagttt ttgcatttat atatctgtta
      721 atagatcaaa aatcatcgct tcgctga
//
