LOCUS       sequence-331442-        9789 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses LIR (LC3-interacting region) mutant NEK9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9789)
  AUTHORS   Yamamoto Y, Chino H, Tsukamoto S, Ode KL, Ueda HR, Mizushima N
  TITLE     NEK9 regulates primary cilia formation by acting as a selective 
            autophagy adaptor for MYH9/myosin IIA.
  JOURNAL   Nat Commun. 2021 Jun 2;12(1):3292. doi: 10.1038/s41467-021-23599-7.
  PUBMED    34078910
REFERENCE   2  (bases 1 to 9789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-2"; volume: "12(1):3292. doi"; pages: " 
            10.1038/s41467-021-23599-7"
COMMENT     Sequence Label: pMRX-IPU-GFP-NEK9W967A
FEATURES             Location/Qualifiers
     source          1..9789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(545..1144)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(628..648)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     1125..1144
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    1162..1714
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1164..1730
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1324..1343)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     1553..1570
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(2801..2812)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             complement(join(4720..5430,5431..5433,5434..5436))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(4720..5430,5431..5433,5434..5436))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4762..4783)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5111..5130
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5370..5391
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5433..5442
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5469..5843
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(5515..5531)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             complement(5853..6269)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(5869..5885)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(5901..5923)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    6334..6691
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     enhancer        7050..7429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(7531..7549)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7617..7721
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7722..7790,7791..8582)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7940..7959)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8746..9334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9235..9254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9488..9505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             join(9635..9789,1..315)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gttgtgggct cttttattga gctcggggag cagaagcgcg cgaacagaag cgagaagcga
       61 actgattggt tagttcaaat aaggcacagg gtcatttcag gtccttgggg caccctggaa
      121 acatctgatg gttctctaga aactgctgag ggctggaccg catctgggga ccatctgttc
      181 ttggccctga gccggggcag gaactgctta ccacagatat cctgtttggc ccatattcag
      241 ctgttccatc tgttcttggc cctgagccgg ggcaggaact gcttaccaca gatatcctgt
      301 ttggcccata ttcagctgtt ccatctgttc ctgaccttga tctgaacttc tctattctca
      361 gttatgtatt tttccatgcc ttgcaaaatg gcgttactta agctagcttg ccaaacctac
      421 aggtggggtc tttcattccc ccctttttct ggagactaaa taaaatcttt tattttatcg
      481 tcgagccatg gggtcgtgcg ctcctttcgg tcgggcgctg cgggtcgtgg ggcgggcggt
      541 cgactcaggc accgggcttg cgggtcatgc accaggtgcg cggtccttcg ggcacctcga
      601 cgtcggcggt gacggtgaag ccgagccgct cgtagaaggg gaggttgcgg ggcgcggagg
      661 tctccaggaa ggcgggcacc ccggcgcgct cggccgcctc cactccgggg agcacgacgg
      721 cgctgcccag acccttgccc tggtggtcgg gcgagacgcc gacggtggcc aggaaccacg
      781 cgggctcctt gggccggtgc ggcgccagga ggccttccat ctgttgctgc gcggccagcc
      841 gggaaccgct caactcggcc atgcgcgggc cgatctcggc gaacaccgcc cccgcttcga
      901 cgctctccgg cgtggtccag accgccaccg cggcgccgtc gtccgcgacc cacaccttgc
      961 cgatgtcgag cccgacgcgc gtgaggaaga gttcttgcag ctcggtgacc cgctcgatgt
     1021 ggcggtccgg gtcgacggtg tggcgcgtgg cggggtagtc ggcgaacgcg gcggcgaggg
     1081 tgcgtacggc ccgggggacg tcgtcgcggg tggcgaggcg caccgtgggc ttgtactcgg
     1141 tcatgtcgac ggttgtggcc atattatcat cgtgtttttc aaaggaaaac cacgtccccg
     1201 tggttcgggg ggcctagacg tttttttaac ctcgactaaa cacatgtaaa gcatgtgcac
     1261 cgaggcccca gatcagatcc catacaatgg ggtaccttct gggcatcctt cagccccttg
     1321 ttgaatacgc ttgaggagag ccatttgact ctttccacaa ctatccaact cacaacgtgg
     1381 cactggggtt gtgccgcctt tgcaggtgta tcttatacac gtggcttttg gccgcagagg
     1441 cacctgtcgc caggtggggg gttccgctgc ctgcaaaggg tcgctacaga cgttgtttgt
     1501 cttcaagaaa cttccagagg aactgcttcc ttcacgacat tcaacagacc ttgcattcct
     1561 ttggcgagag gggaaagacc cctaggaatg ctcgtcaaga agacagggcc aggtttccgg
     1621 gccctcacat tgccaaaaga cggcaatatg gtggaaaata acatatagac aaacgcacac
     1681 cggccttatt ccaagcggct tcggccagta acgttagggg ggggggcgga atttacgtag
     1741 cggccgctct cgagacccga attcctagag gctgggtcta caggagtctg ttcccaggag
     1801 gcatgcggaa tctgaatcta agtcaggctt tggatccatt tccatctctt cctttgctgt
     1861 ctgagttcct ttggaatgca tccccacctg ctgccctcct tctaatttct tgttcaactt
     1921 ctgcagttgg gtaaaaatct ggaggttttc ttgctgtagc ttctgttgtt cagccagaca
     1981 ctttaacacc agaccctgca atctctcaac ctccacctgc agagagctgc acgcacacgc
     2041 aggaggagtc agtggtgttc ccttcccagc acaggttact gcaggattca gccgaggtga
     2101 cgaggcttct acttggggtt tgtgttccaa aggagcttca gaggccactt tgagtccttg
     2161 cagctcttca tagggcaggg tatctttctc agattctgaa aacgctgcac tgagaggaga
     2221 tgggctgtca ggcatgggga taaattctgc attttccagc tcctttcgaa gccagccagg
     2281 acaggagctg ctggccccat tactgttccc catggcctct gtgggactga ttaaaccttc
     2341 cattcctcgg tctgcttcca ttgttcctcg gaagcctcca cttgggtcag gagtttcaga
     2401 ttcctgctga ctgtcctctt cttcaccacc gccgcccccg ccgcctcctc ccgggctaga
     2461 gctctgaaac acagttccaa tggataagcc actgctattg gaacggatgg tcttagaatt
     2521 caatactttc tcaacgatga gaatggtatg ccatccacgg caagacaggt ccgggacatg
     2581 atgcagagat ccaaaaatag gccgaggcca tgaggtacag atatcagagc catgtggtct
     2641 ctctgtgggg gtcattgcca ggcggccatt accaccattg ccccaggcaa aaatgtgatt
     2701 atcatcagtg gcagcaatgg taaactcatc accgcaggag accctgatca cttgcttccc
     2761 accaaggggt ccccccaaca ggttgattcc cagacgcttc ttgtagttcc caacgcccag
     2821 ctgcccacac ttgttgcagc caaaggtcag cagccggcct cgctcatcaa tagcagctgt
     2881 gtgagtcttg cctggggcaa tggtacggat cttataaaag gacaactgtt tggccaaggt
     2941 aaaggacgtt gtgtagggaa cttcatggta tgcttcatgg ttgataattc ccgacatgca
     3001 ctgattcaga cccagcttat tgaattcatt gagtccacag gccagcactt tgcctgactg
     3061 ggtcaacaga aatgtcccat cacagccaca ttgaactgca acaataatca aggccttggg
     3121 aacatccacc ttttgtggtg tataataatc ctcttctgaa tccaaaccca gtcgtccata
     3181 ttcgccacag ccccaagaat agacttcctt gtttcgtgtc agaaccacca catgattatc
     3241 tccacaggag acctgctcca ctggattgct gaggaagaag ttcagctgca tgggttctag
     3301 cacttcaggg ccagcaactt tgtccacccc catgcagcca taataatctg atccgaaggc
     3361 atagagctga ccctcatcag tcacacagac agtgaaatca tcaccacatg acacctgacg
     3421 gatagctttg ccttgcaact tttccacatg ctttggctgt cgataggagg ctttgtctcc
     3481 atggcccagc tgaccatgga gtttagtgcc tccttgcatg ttcacccaag tgtacagttc
     3541 cttctccact gtgaccacag caaagtgggt attccctgca cagacctgcc gggcactaca
     3601 gccactcttg ataacatcca gtttctgggg ggtggatttt ccaccacccc aaacatagac
     3661 ttcactggtt cgtgatgtta ctacagcaat gggtgcttca gtcacagtgc ttgaccttgg
     3721 tctctttgta ggtgcattaa gcagagtgac tttttcctcc atctctctcc tgcgtttcct
     3781 gagaagaggg cgatctagaa gttcatctgc agtaggtctc tgctcaggat cctggtcaag
     3841 gcacgaatga accatttgga tcaattccaa agagtactgg ctagagtcaa cttccatggc
     3901 ccgaattcct tgcacgatct tcacacacag gttaagtggg tttgtagcat caaacgtcct
     3961 ctttaaggta agcagttcaa aaatgacgca gccaactgcc cagatatcag acttgaaatt
     4021 gtactttact ccttgacaga gctctggaga catgtaatat ggggttccca caagcgtctc
     4081 agccatggaa tactcagaat taagtttctt tgctaggcca taatctccaa gttttatcag
     4141 gtttgccttg gtcagaaaaa tatttaatgt ctttatatct ctatgaagga ttccagcttt
     4201 atggatgcag ctcactgctg aaacaatctg aaataggtac cacaccacca tctcttcctc
     4261 aaacaacttg tccttctgac gaaggatttt gtcatacagg ttccctccat tacaatattc
     4321 cagctcaatc agcagcgtgg tattgtccat gaagtgattg tagtaggcaa taatgttgtc
     4381 gtgctgcagc agtgccagaa taactatctc attcaaggca tcacgacgtt ccttctcaga
     4441 cagccgggtc aaatcgactt ccttccacac aaccagtgag tcatcctcgg tgcggcggta
     4501 cagcgtggct tccccgaagg cgccgcggcc caggacgcgg atggggatgt agtgcagttc
     4561 ctcctgctcc gccgcgccgc cgccggctcg cggcccctga ctggcgctag gccccggact
     4621 cgagtccccg caacccccgg actcgctccc aaagtccgag ttgatggaat cgcagtgtcg
     4681 ctcgtactcg cccagcaccg aggatcccga gcctgaaccc ttgtacagct cgtccatgcc
     4741 gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt
     4801 ggggtctttg ctcagggcgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg
     4861 gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc
     4921 gatgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttgt cggccatgat
     4981 atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc
     5041 cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac
     5101 ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta
     5161 gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct
     5221 gaagcactgc acgccgtagg tcagggtggt cacgagggtg ggccagggca cgggcagctt
     5281 gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc
     5341 gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac
     5401 caccccggtg aacagctcct cgcccttgct caccatggtg gcaagcttta tatcgatggc
     5461 agatccggca gtctagagga tggtccaccc ccggggtcgg cagccttcac gtgggcggcg
     5521 tgtatccaag ctgcgatgcc gtctactttg agggcggtgg gggtggtcag caggactgtg
     5581 taaggtcctt tccagcgagg ttctaggttc ttagtctggt gtcggcggac ccacactgtg
     5641 tcgccgactc ggtaagggtg aggtaccacc ggtcggtcca gttgttcttg gtaggctgcc
     5701 gccagaggtc tccagacttc gtgctggact aagtagagag cctgtaagtg agcttggaga
     5761 gaggggctgt tagtaactct tgtcatgtca gggtcaggga agtttacaag gggcgggggt
     5821 gccccatata agatctcata tggccatatg ggggcgccta gagaaggagt gagggctgga
     5881 taaagggagg atcgaggcgg ggtcgaacga ggaggttcaa gggggagaga cggggcggat
     5941 ggaggaagag gaggcggagg cttagggtgt acaaagggct tgacccaggg aggggggtca
     6001 aaagccaagg cttcccaggt cacgatgtag gggacctggt ctgggtgtcc atgcgggcca
     6061 ggtgaaaaga ccttgatctt aacctgggtg atgaggtctc ggttaaaggt gccgtctcgc
     6121 ggccatccga cgttaaaggt tggccattct gcagagcaga aggtaaccca acgtctcttc
     6181 ttgacatcta ccgactggtt gtgagcgatc cgctcgacat ctttccagtg acctaaggtc
     6241 aaacttaagg gagtggtaac agtctggccc gggcccatat tttcagacaa atacagaaac
     6301 acagtcagac agagacaaca cagaacgatg ctgcagcaga caagacgcgc ggcgcggctt
     6361 cggtcccaaa ccgaaagcaa aaattcagac ggaggcggga actgttttag gttctcgtct
     6421 cctaccagaa ccacatatcc ctcctctaag gggggtgcac caaagagtcc aaaacgatcg
     6481 ggatttttgg actcaggtcg ggccacaaaa acggcccccg aagtccctgg gacgtctccc
     6541 agggttgcgg ccgggtgttc cgaactcgtc agttccacca cgggtccgcc agatacagag
     6601 ctagttagct aactagtacc gacgcaggcg cataaaatca gtcatagaca ctagacaatc
     6661 ggacagacac agataagttg ctggccagct tacctcccgg tggtgggtcg gtggtccctg
     6721 ggcaggggtc tcccgatccc ggacgagccc ccaaatgaaa gacccccgct gacgggtagt
     6781 caatcactca gaggagaccc tcccaaggaa cagcgagacc acaagtcgga tgcaactgca
     6841 agagggttta ttggatacac gggtacccgg gcgactcagt caatcggagg actggcgcgc
     6901 cgagtgaggg gttgtgggct cttttattga gctcggggag cagaagcgcg cgaacagaag
     6961 cgagaagcga actgattggt tagttcaaat aaggcacagg gtcatttcag gtccttgggg
     7021 caccctggaa acatctgatg gttctctagc atggtaatag cgatgactaa tacgtagatg
     7081 tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat
     7141 ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc
     7201 aagtgggcag tttaccgtaa atacttcacc cattgacgtc aatggaaagt ccctattggc
     7261 gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag
     7321 ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact
     7381 aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtca acatggcggt
     7441 aatgttggac atgagccaat ataaatgtac atattatgat atggatacaa cgtatgcaat
     7501 gggccaagct cctatagtga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     7561 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     7621 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     7681 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     7741 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     7801 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     7861 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     7921 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     7981 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     8041 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     8101 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     8161 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     8221 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     8281 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     8341 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     8401 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     8461 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     8521 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     8581 aactgtcaga ccaagtttac tcatatatac tttagattga tttgcggccg gccgcaaaag
     8641 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8701 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8761 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8821 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8881 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     8941 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     9001 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     9061 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     9121 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     9181 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     9241 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     9301 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9361 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9421 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     9481 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     9541 ccccgcgcgt tggccgattc attaatgcaa ctatggccat ttaatgtaaa tacttaagaa
     9601 aaaaaaccaa attaattttg atacatgctg catgtgaaag acccccgctg acgggtagtc
     9661 aatcactcag aggagaccct cccaaggaac agcgagacca caagtcggat gcaactgcaa
     9721 gagggtttat tggatacacg ggtacccggg cgactcagtc aatcggagga ctggcgcccc
     9781 gagtgaggg
//
