LOCUS       sequence-33145-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  TITLE     pFA6a-TRP1-PGAL1-HBTH
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFA6a-TRP1-PGAL1-HBTH
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(58..245)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     166..190
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(272..289)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(311..331)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(584..601)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(645..668)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 gcttcgtacg ctgcaggtcg acggatctat attaccctgt tatccctagc ggatctgccg
       61 gtagaggtgt ggtcaataag agcgacctca tactatacct gagaaagcaa cctgacctac
      121 aggaaagagt tactcaagaa taagaatttt cgttttaaaa cctaagagtc actttaaaat
      181 ttgtatacac ttattttttt tataacttat ttaataataa aaatcataaa tcataagaaa
      241 ttcgcttatt tagaagtggc gcgccggctc catgatggtg gtgatgatgt gaacccctca
      301 tccctaactc tccttgaaag tacagattct cgctggcggt tgtgggtata tcgtagtcgc
      361 cgatcttgat tagaccttga ccaccttgaa cagcatctct ttccttaact aacacctttt
      421 caacttttcc gtcagtagga gcgttaattt ctgtctccat tttcatagcc tccagcacca
      481 gaactgtttg accagcttta acagtgtcgc cttcttttac cagtatctta gaaacggtac
      541 cagcaagagg ggcagggatt tcaccttcac cggcctttcc agcatgatgg tggtgatgat
      601 gcatgttaat taaagacatt ttgagatccg ggttttttct ccttgacgtt aaagtataga
      661 ggtatattaa caattttttg ttgatacttt tattacattt gaataagaag taatacaaac
      721 cgaaaatgtt gaaagtatta gttaaagtgg ttatgcagtt tttgcattta tatatctgtt
      781 aatagatcaa aaatcatcgc ttcgctgatt aattacccca gaaataannc taaaaaacta
      841 atcgcattat catcctatgg ttgttaattt gattcgttca tttgaaggtt tgtggggcca
      901 ggttactgcc aatttttcct cttcataacc ataaaagcta gt
//
