LOCUS       sequence-331458-       12735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MYH9 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12735)
  AUTHORS   Yamamoto Y, Chino H, Tsukamoto S, Ode KL, Ueda HR, Mizushima N
  TITLE     NEK9 regulates primary cilia formation by acting as a selective 
            autophagy adaptor for MYH9/myosin IIA.
  JOURNAL   Nat Commun. 2021 Jun 2;12(1):3292. doi: 10.1038/s41467-021-23599-7.
  PUBMED    34078910
REFERENCE   2  (bases 1 to 12735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-2"; volume: "12(1):3292. doi"; pages: " 
            10.1038/s41467-021-23599-7"
COMMENT     Sequence Label: pMRX-IPU-GFP-MYH9
FEATURES             Location/Qualifiers
     source          1..12735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    359..716
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             781..1197
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1127..1149
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1165..1181
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1207..1581
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1519..1535
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      1608..1617
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1614..1616,1617..1619,1620..2330)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1659..1680)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1920..1939)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2267..2288
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8266..8832
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    8282..8834
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8426..8443)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8653..8672
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8852..9451
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8852..8871)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9348..9368
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9681..10150
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(10280..10297)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10451..11039)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10531..10550)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11203..11994,11995..12063))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11826..11845
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12064..12168)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12236..12254
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        12356..12735
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta
       61 tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct
      121 caataaaaga gcccacaacc cctcactcgg cgcgccagtc ctccgattga ctgagtcgcc
      181 cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt
      241 tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg
      301 ggctcgtccg ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggtaa
      361 gctggccagc aacttatctg tgtctgtccg attgtctagt gtctatgact gattttatgc
      421 gcctgcgtcg gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg
      481 acgagttcgg aacacccggc cgcaaccctg ggagacgtcc cagggacttc gggggccgtt
      541 tttgtggccc gacctgagtc caaaaatccc gatcgttttg gactctttgg tgcacccccc
      601 ttagaggagg gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg
      661 tctgaatttt tgctttcggt ttgggaccga agccgcgccg cgcgtcttgt ctgctgcagc
      721 atcgttctgt gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa tatgggcccg
      781 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
      841 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
      901 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
      961 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
     1021 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
     1081 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
     1141 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccccc
     1201 atatggccat atgagatctt atatggggca cccccgcccc ttgtaaactt ccctgaccct
     1261 gacatgacaa gagttactaa cagcccctct ctccaagctc acttacaggc tctctactta
     1321 gtccagcacg aagtctggag acctctggcg gcagcctacc aagaacaact ggaccgaccg
     1381 gtggtacctc acccttaccg agtcggcgac acagtgtggg tccgccgaca ccagactaag
     1441 aacctagaac ctcgctggaa aggaccttac acagtcctgc tgaccacccc caccgccctc
     1501 aaagtagacg gcatcgcagc ttggatacac gccgcccacg tgaaggctgc cgaccccggg
     1561 ggtggaccat cctctagact gccggatctg ccatcgatat aaagcttgcc accatggtga
     1621 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     1681 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     1741 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     1801 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     1861 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     1921 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     1981 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     2041 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     2101 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     2161 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     2221 gcacccagtc caaactgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     2281 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag ggttcaggct
     2341 cgggatccgc acagcaagct gccgataagt atctctatgt ggataaaaac ttcatcaaca
     2401 atccgctggc ccaggccgac tgggctgcca agaagctggt atgggtgcct tccgacaaga
     2461 gtggctttga gccagccagc ctcaaggagg aggtgggcga agaggccatc gtggagctgg
     2521 tggagaatgg gaagaaggtg aaggtgaaca aggatgacat ccagaagatg aacccgccca
     2581 agttctccaa ggtggaggac atggcagagc tcacgtgcct caacgaagcc tcggtgctgc
     2641 acaacctcaa ggagcgttac tactcagggc tcatctacac ctattcaggc ctgttctgtg
     2701 tggtcatcaa tccttacaag aacctgccca tctactctga agagattgtg gaaatgtaca
     2761 agggcaagaa gaggcacgag atgccccctc acatctatgc catcacagac accgcctaca
     2821 ggagtatgat gcaagaccga gaagatcaat ccatcttgtg cactggtgaa tctggagctg
     2881 gcaagacgga gaacaccaag aaggtcatcc agtatctggc gtacgtggcg tcctcgcaca
     2941 agagcaagaa ggaccagggc gagctggagc ggcagctgct gcaggccaac cccatcctgg
     3001 aggccttcgg gaacgccaag accgtgaaga atgacaactc ctcccgcttc ggcaaattca
     3061 ttcgcatcaa ctttgatgtc aatggctaca ttgttggagc caacattgag acttatcttt
     3121 tggagaaatc tcgtgctatc cgccaagcca aggaagaacg gaccttccac atcttctatt
     3181 atctcctgtc tggggctgga gagcacctga agaccgatct cctgttggag ccgtacaaca
     3241 aataccgctt cctgtccaat ggacacgtca ccatccccgg gcagcaggac aaggacatgt
     3301 tccaggagac catggaggcc atgaggatta tgggcatccc agaagaggag caaatgggcc
     3361 tgctgcgggt catctcaggg gttcttcagc tcggcaacat cgtcttcaag aaggagcgga
     3421 acactgacca ggcgtccatg cccgacaaca cagctgccca aaaggtgtcc catctcttgg
     3481 gtatcaatgt gaccgatttc accagaggaa tcctcacccc gcgcatcaag gtgggacggg
     3541 attacgtcca gaaggcgcag actaaagagc aggctgactt tgccatcgag gccttggcca
     3601 aggcgaccta tgagcggatg ttccgctggc tggtgctgcg catcaacaag gctctggaca
     3661 agaccaagag gcagggcgcc tccttcatcg ggatcctgga cattgccggc ttcgagatct
     3721 ttgatctgaa ctcgtttgag cagctgtgca tcaattacac caatgagaag ctgcagcagc
     3781 tcttcaacca caccatgttc atcctggagc aggaggagta ccagcgcgag ggcatcgagt
     3841 ggaacttcat cgactttggc ctcgacctgc agccctgcat cgacctcatt gagaagccag
     3901 caggcccccc gggcattctg gccctgctgg acgaggagtg ctggttcccc aaagccaccg
     3961 acaagagctt cgtggagaag gtgatgcagg agcagggcac ccaccccaag ttccagaagc
     4021 ccaagcagct gaaggacaaa gctgatttct gcattatcca ctatgccggc aaggtggatt
     4081 acaaagctga cgagtggctg atgaagaaca tggatcccct gaatgacaac atcgccacac
     4141 tgctccacca gtcctctgac aagtttgtct cggagctgtg gaaggatgtg gaccgcatca
     4201 tcggcctgga ccaggtggcc ggcatgtcgg agaccgcact gcccggggcc ttcaagacgc
     4261 ggaagggcat gttccgcact gtggggcagc tttacaagga gcagctggcc aagctgatgg
     4321 ctacgctgag gaacacgaac cccaactttg tccgctgcat catccccaac cacgagaaga
     4381 aggccggcaa gctggacccg catctcgtgc tggaccagct gcgctgcaac ggtgttctcg
     4441 agggcatccg tatctgccgc cagggcttcc ccaacagggt ggtcttccag gagtttcggc
     4501 agagatatga gatcctgact ccaaactcca ttcccaaggg tttcatggac gggaagcagg
     4561 cgtgcgtgct catgataaaa gccctggagc tcgacagcaa cctgtaccgc attggccaga
     4621 gcaaagtctt cttccgtgcc ggtgtgctgg cccacctgga ggaggagcga gacctgaaga
     4681 tcaccgacgt catcataggg ttccaggcct gctgcagggg ctacctggcc aggaaagcat
     4741 ttgccaagcg gcagcagcag cttaccgcca tgaaggtcct ccagcggaac tgcgctgcct
     4801 acctgaagct gcggaactgg cagtggtggc ggctcttcac caaggtcaag ccgctgctgc
     4861 aggtgagccg gcaggaggag gagatgatgg ccaaggagga ggagctggtg aaggtcagag
     4921 agaagcagct ggctgcggag aacaggctca cggagatgga gacgctgcag tctcagctca
     4981 tggcagagaa attgcagctg caggagcagc tccaggcaga aaccgagctg tgtgccgagg
     5041 ctgaggagct ccgggcccgc ctgaccgcca agaagcagga attagaagag atctgccatg
     5101 acctagaggc cagggtggag gaggaggagg agcgctgcca gcacctgcag gcggagaaga
     5161 agaagatgca gcagaacatc caggagcttg aggagcagct ggaggaggag gagagcgccc
     5221 ggcagaagct gcagctggag aaggtgacca ccgaggcgaa gctgaaaaag ctggaggagg
     5281 agcagatcat cctggaggac cagaactgca agctggccaa ggaaaagaaa ctgctggaag
     5341 acagaatagc tgagttcacc accaacctca cagaagagga ggagaaatct aagagcctcg
     5401 ccaagctcaa gaacaagcat gaggcaatga tcactgactt ggaagagcgc ctccgcaggg
     5461 aggagaagca gcgacaggag ctggagaaga cccgccggaa gctggaggga gactccacag
     5521 acctcagcga ccagatcgcc gagctccagg cccagatcgc ggagctcaag atgcagctgg
     5581 ccaagaaaga ggaggagctc caggccgccc tggccagagt ggaagaggaa gctgcccaga
     5641 agaacatggc cctcaagaag atccgggagc tggaatctca gatctctgaa ctccaggaag
     5701 acctggagtc tgagcgtgct tccaggaata aagctgagaa gcagaaacgg gaccttgggg
     5761 aagagctaga ggcgctgaaa acagagttgg aggacacgct ggattccaca gctgcccagc
     5821 aggagctcag gtcaaaacgt gagcaggagg tgaacatcct gaagaagacc ctggaggagg
     5881 aggccaagac ccacgaggcc cagatccagg agatgaggca gaagcactca caggccgtgg
     5941 aggagctggc ggagcagctg gagcagacga agcgggtgaa agcaaacctc gagaaggcaa
     6001 agcagactct ggagaacgag cggggggagc tggccaacga ggtgaaggtg ctgctgcagg
     6061 gcaaagggga ctcggagcac aagcgcaaga aagtggaggc gcagctgcag gagctgcagg
     6121 tcaagttcaa cgagggagag cgcgtgcgca cagagctggc cgacaaggtc accaagctgc
     6181 aggtggagct ggacaacgtg accgggcttc tcagccagtc cgacagcaag tccagcaagc
     6241 tcaccaagga cttctccgcg ctggagtccc agctgcagga cactcaggag ctgctgcagg
     6301 aggagaaccg gcagaagctg agcctgagca ccaagctcaa gcaggtggag gacgagaaga
     6361 attccttccg ggagcagctg gaggaggagg aggaggccaa gcacaacctg gagaagcaga
     6421 tcgccaccct ccatgcccag gtggccgaca tgaaaaagaa gatggaggac agtgtggggt
     6481 gcctggaaac tgctgaggag gtgaagagga agctccagaa ggacctggag ggcctgagcc
     6541 agcggcacga ggagaaggtg gccgcctacg acaagctgga gaagaccaag acgcggctgc
     6601 agcaggagct ggacgacctg ctggtggacc tggaccacca gcgccagagc gcgtgcaacc
     6661 tggagaagaa gcagaagaag tttgaccagc tcctggcgga ggagaagacc atctctgcca
     6721 agtatgcaga ggagcgcgac cgggctgagg cggaggcccg agagaaggag accaaggctc
     6781 tgtcgctggc ccgggccctg gaggaagcca tggagcagaa ggcggagctg gagcggctca
     6841 acaagcagtt ccgcacggag atggaggacc ttatgagctc caaggatgat gtgggcaaga
     6901 gtgtccacga gctggagaag tccaagcggg ccctagagca gcaggtggag gagatgaaga
     6961 cgcagctgga agagctggag gacgagctgc aggccaccga agatgccaag ctgcggttgg
     7021 aggtcaacct gcaggccatg aaggcccagt tcgagcggga cctgcagggc cgggacgagc
     7081 agagcgagga gaagaagaag cagctggtca gacaggtgcg ggagatggag gcagagctgg
     7141 aggacgagag gaagcagcgc tcgatggcag tggccgcccg gaagaagctg gagatggacc
     7201 tgaaggacct ggaggcgcac atcgactcgg ccaacaagaa ccgggacgaa gccatcaaac
     7261 agctgcggaa gctgcaggcc cagatgaagg actgcatgcg cgagctggat gacacccgcg
     7321 cctctcgtga ggagatcctg gcccaggcca aagagaacga gaagaagctg aagagcatgg
     7381 aggccgagat gatccagttg caggaggaac tggcagccgc ggagcgtgcc aagcgccagg
     7441 cccagcagga gcgggatgag ctggctgacg agatcgccaa cagcagcggc aaaggagccc
     7501 tggcgttaga ggagaagcgg cgtctggagg cccgcatcgc ccagctggag gaggagctgg
     7561 aggaggagca gggcaacacg gagctgatca acgaccggct gaagaaggcc aacctgcaga
     7621 tcgaccagat caacaccgac ctgaacctgg agcgcagcca cgcccagaag aacgagaatg
     7681 ctcggcagca gctggaacgc cagaacaagg agcttaaggt caagctgcag gagatggagg
     7741 gcactgtcaa gtccaagtac aaggcctcca tcaccgccct cgaggccaag attgcacagc
     7801 tggaggagca gctggacaac gagaccaagg agcgccaggc agcctgcaaa caggtgcgtc
     7861 ggaccgagaa gaagctgaag gatgtgctgc tgcaggtgga tgacgagcgg aggaacgccg
     7921 agcagtacaa ggaccaggcc gacaaggcat ctacccgcct gaagcagctc aagcggcagc
     7981 tggaggaggc cgaagaggag gcccagcggg ccaacgcctc ccgccggaaa ctgcagcgcg
     8041 agctggagga cgccactgag acggccgatg ccatgaaccg cgaagtcagc tccctaaaga
     8101 acaagctcag gcgcggggac ctgccgtttg tcgtgccccg ccgaatggcc cggaaaggcg
     8161 ccggggatgg ctccgacgaa gaggtagatg gcaaagcgga tggggctgag gccaaacctg
     8221 ccgaataaga attcgggtct cgagtaaagc ggccgctacg taaattccgc ccccccccct
     8281 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     8341 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     8401 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     8461 gtgaaggaag cagttcctct ggaagtttct tgaagacaaa caacgtctgt agcgaccctt
     8521 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     8581 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     8641 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     8701 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     8761 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     8821 acacgatgat aatatggcca caaccgtcga catgaccgag tacaagccca cggtgcgcct
     8881 cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta
     8941 ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca
     9001 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     9061 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     9121 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     9181 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     9241 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     9301 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     9361 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     9421 ctggtgcatg acccgcaagc ccggtgcctg agtcgaccgc ccgccccacg acccgcagcg
     9481 cccgaccgaa aggagcgcac gaccccatgg ctcgacgata aaataaaaga ttttatttag
     9541 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
     9601 aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag
     9661 gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     9721 ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc
     9781 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
     9841 cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
     9901 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
     9961 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc
    10021 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
    10081 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
    10141 gggtctttca catgcagcat gtatcaaaat taatttggtt ttttttctta agtatttaca
    10201 ttaaatggcc atagttgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
    10261 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    10321 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    10381 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    10441 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    10501 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    10561 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    10621 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    10681 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    10741 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    10801 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    10861 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
    10921 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    10981 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    11041 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    11101 ggattttggt catgagatta tcaaaaagga tcttcaccta gatccttttg cggccggccg
    11161 caaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    11221 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    11281 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    11341 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    11401 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    11461 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    11521 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    11581 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    11641 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    11701 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    11761 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac
    11821 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    11881 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    11941 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    12001 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    12061 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    12121 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    12181 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    12241 gcgtatcacg aggccctttc gtcttcacta taggagcttg gcccattgca tacgttgtat
    12301 ccatatcata atatgtacat ttatattggc tcatgtccaa cattaccgcc atgttgacat
    12361 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
    12421 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
    12481 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
    12541 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
    12601 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
    12661 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
    12721 atcgctatta ccatg
//
