LOCUS       sequence-33146-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, 
            Huang L, Kaiser P
  TITLE     A tandem affinity tag for two-step purification under fully 
            denaturing conditions: application in ubiquitin profiling and 
            protein complex identification combined with in vivocross-linking.
  JOURNAL   Mol Cell Proteomics. 2006 Apr . 5(4):737-48.
  PUBMED    16432255
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2006 Apr . 5(4):737-48."
COMMENT     Sequence Label: YEpURA-CUP1-HBTubiquitin
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     133..151
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(205..429)
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="QIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFA
                     GKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(475..495)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(748..765)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gaaaaaaaag aaaaatttga aatataaata acgttcttaa tactaacata actattaaaa
       61 aaaataaata gggacctaga cttcaggttg tctaactcct tccttttcgg ttagagcgga
      121 tgtgggagga gggcgtgaat gtaagcgtga cataactaat tacatgatat cgacaaagga
      181 aaaggggcct gtttaggatc ctcaaccacc tcttagtctt aagacaagat gtaaggtgga
      241 ctccttctga atgttgtaat cagacagcgt tctaccgtct tctagctgct taccggcaaa
      301 gatcaatctt tgttgatctg gagggatacc ttccttgtct tgaattttcg acttaacgtt
      361 gtcgatggta tcggaagatt caacttccaa tgttatggtt ttaccggtca aagtcttgac
      421 gaaaatctgt ctagaagcct cgtcatgttg agctagtgcg gcggttttta actctgattg
      481 aaagtacaga ttctcgctgg cggttgtggg tatatcgtag tcgccgatct tgattagacc
      541 ttgaccacct tgaacagcat ctctttcctt aactaacacc ttttcaactt ttccgtcagt
      601 aggagcgtta atttctgtct ccattttcat agcctccagc accagaactg tttgaccagc
      661 tttaacagtg tcaccttctt ttaccagtat cttagaaacg gtaccagcaa gaggggcagg
      721 gatttcacct tcaccggcct ttccagcatg atggtggtga tgatgtgaac ccctcataat
      781 ggatccttta tgtgatgatt gattgattga ttgtacagtt t
//
