LOCUS       sequence-33148-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Basu S, Mehreja R, Thiberge S, Chen MT, Weiss R
  TITLE     Spatiotemporal control of gene expression with pulse-generating 
            networks.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Apr 27. 101(17):6355-60.
  PUBMED    15096621
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Apr 27. 101(17):6355-60."
COMMENT     Sequence Label: pPSSUB-101
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(32..134)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     200..218
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     RBS             350..361
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(398..426)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cggtggtata tccngtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
       61 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
      121 ttacgtgccg atcaacgtct cattttcgcc agatatcgac gtctaagaaa ccattattat
      181 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc atctcttgtc
      241 acactattgt atcgctggga atacaattac ttaacataag cacctgtagg atcgtacagg
      301 tttacgcaag aaaatggttt gttatagtcg aatacatctg gcggtgataa aagaggagaa
      361 attaagcatg cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      421 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
      481 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
      541 aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat
      601 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
      661 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
      721 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
      781 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa
      841 gaatggaatc aaagttaact tcaaaattag aca
//
