LOCUS       sequence-331487-        8742 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mediated knock-in of sgRNA precursor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8742)
  AUTHORS   Gao N, Hu J, He B, Ji Z, Hu X, Huang J, Wei Y, Peng J, Wei Y, Zhou 
            Y, Shen X, Li H, Feng X, Xiao Q, Shi L, Sun Y, Zhou C, Zhou H, Yang 
            H
  TITLE     Endogenous promoter-driven sgRNA for monitoring the expression of 
            low-abundance transcripts and lncRNAs.
  JOURNAL   Nat Cell Biol. 2021 Jan;23(1):99-108. doi: 
            10.1038/s41556-020-00610-9. Epub 2021 Jan 4.
  PUBMED    33398178
REFERENCE   2  (bases 1 to 8742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-020-00610-9"; journalName: "Nat Cell Biol"; date: 
            "2021-01"; volume: "23"; issue: "1"; pages: "99-108"
COMMENT     Sequence Label: 
            AAV-U6-sgRNA-EF1-alpha-sakkhCas9-HA-NLS-polyA-CBh-EGFP-polyA
FEATURES             Location/Qualifiers
     source          1..8742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          163..390
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             join(409..411,412..414,415..1128)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(409..411,412..414,415..1125)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(454..475)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(715..734)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1062..1083
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1135..1342
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   1351..1491
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   1351..1480
                     /label=AAV2 ITR
     rep_origin      1566..2021
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1653..1672)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1863..1884
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2038..2057)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2157..2179
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2217..2235)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2303..2407
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2408..2476,2477..3268)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2626..2645)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3439..4027
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3928..3947
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   4089..4218
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        4231..4479
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4231..4251
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4402..4421
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4500..4575
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     promoter        4637..4848
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             4901..4921
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      4928..4937
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8096..8122
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8102..8128
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             8174..8194
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8198..8332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8235..8254)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8289..8308
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     enhancer        8340..8625
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        join(8627..8742,1..162)
                     /label=chicken beta-actin promoter
ORIGIN
        1 ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga
       61 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
      121 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc
      181 cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
      241 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagctg
      301 agcaagaggt aagggtttaa gggatggttg gttggtgggg tattaatgtt taattacctg
      361 gagcacctgc ctgaaatcac tttttttcag gttggaccgg ccgctagcat ggtgagcaag
      421 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      481 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      541 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      601 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
      661 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      721 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      781 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      841 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
      901 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
      961 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1021 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1081 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagg taccctgtgc
     1141 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     1201 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     1261 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     1321 agaatagcag gcatgctggg gagcggccgc aggaacccct agtgatggag ttggccactc
     1381 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     1441 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
     1501 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
     1561 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     1621 gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct
     1681 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     1741 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     1801 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     1861 tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga
     1921 tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa
     1981 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
     2041 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     2101 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     2161 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     2221 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     2281 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     2341 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     2401 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     2461 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     2521 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     2581 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     2641 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     2701 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     2761 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     2821 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     2881 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     2941 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     3001 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     3061 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     3121 tggaagccgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     3181 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     3241 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     3301 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     3361 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     3421 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3481 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3541 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     3601 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3661 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3721 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3781 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3841 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3901 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3961 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     4021 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     4081 tcacatgtcc tgcaggcagc tgcgcgctcg ctcgctcact gaggccgccc gggcgtcggg
     4141 cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact
     4201 ccatcactag gggttcctgc ggcctctaga gagggcctat ttcccatgat tccttcatat
     4261 ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac tgtaaacaca
     4321 aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt
     4381 ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat
     4441 ttcttggctt tatatatctt gtggaaagga cgaaacaccg gagaccacgg caggtctcag
     4501 ttatagtact ctggaaacag aatctactat aacaaggcaa aatgccgtgt ttatctcgtc
     4561 aacttgttgg cgagattttt gacgcgtcta gactaggtct tgaaaggagt gggaattggc
     4621 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     4681 ggggtcggca attgatccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     4741 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     4801 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagga ccggtcgcgc
     4861 caccatgaag cggactgctg atggcagtga atttgagtcc ccaaagaaga agagaaaggt
     4921 ggggatcgcc accatggcca agcggaacta catcctgggc ctggacatcg gcatcaccag
     4981 cgtgggctac ggcatcatcg actacgagac acgggacgtg atcgatgccg gcgtgcggct
     5041 gttcaaagag gccaacgtgg aaaacaacga gggcaggcgg agcaagagag gcgccagaag
     5101 gctgaagcgg cggaggcggc atagaatcca gagagtgaag aagctgctgt tcgactacaa
     5161 cctgctgacc gaccacagcg agctgagcgg catcaacccc tacgaggcca gagtgaaggg
     5221 cctgagccag aagctgagcg aggaagagtt ctctgccgcc ctgctgcacc tggccaagag
     5281 aagaggcgtg cacaacgtga acgaggtgga agaggacacc ggcaacgagc tgtccaccaa
     5341 agagcagatc agccggaaca gcaaggccct ggaagagaaa tacgtggccg aactgcagct
     5401 ggaacggctg aagaaagacg gcgaagtgcg gggcagcatc aacagattca agaccagcga
     5461 ctacgtgaaa gaagccaaac agctgctgaa ggtgcagaag gcctaccacc agctggacca
     5521 gagcttcatc gacacctaca tcgacctgct ggaaacccgg cggacctact atgagggacc
     5581 tggcgagggc agccccttcg gctggaagga catcaaagaa tggtacgaga tgctgatggg
     5641 ccactgcacc tacttccccg aggaactgcg gagcgtgaag tacgcctaca acgccgacct
     5701 gtacaacgcc ctgaacgacc tgaacaatct cgtgatcacc agggacgaga acgagaagct
     5761 ggaatattac gagaagttcc agatcatcga gaacgtgttc aagcagaaga agaagcccac
     5821 cctgaagcag atcgccaaag aaatcctcgt gaacgaagag gatattaagg gctacagagt
     5881 gaccagcacc ggcaagcccg agttcaccaa cctgaaggtg taccacgaca tcaaggacat
     5941 taccgcccgg aaagagatta ttgagaacgc cgagctgctg gatcagattg ccaagatcct
     6001 gaccatctac cagagcagcg aggacatcca ggaagaactg accaatctga actccgagct
     6061 gacccaggaa gagatcgagc agatctctaa tctgaagggc tataccggca cccacaacct
     6121 gagcctgaag gccatcaacc tgatcctgga cgagctgtgg cacaccaacg acaaccagat
     6181 cgctatcttc aaccggctga agctggtgcc caagaaggtg gacctgtccc agcagaaaga
     6241 gatccccacc accctggtgg acgacttcat cctgagcccc gtcgtgaaga gaagcttcat
     6301 ccagagcatc aaagtgatca acgccatcat caagaagtac ggcctgccca acgacatcat
     6361 tatcgagctg gcccgcgaga agaactccaa ggacgcccag aaaatgatca acgagatgca
     6421 gaagcggaac cggcagacca acgagcggat cgaggaaatc atccggacca ccggcaaaga
     6481 gaacgccaag tacctgatcg agaagatcaa gctgcacgac atgcaggaag gcaagtgcct
     6541 gtacagcctg gaagccatcc ctctggaaga tctgctgaac aaccccttca actatgaggt
     6601 ggaccacatc atccccagaa gcgtgtcctt cgacaacagc ttcaacaaca aggtgctcgt
     6661 gaagcaggaa gaaaacagca agaagggcaa ccggacccca ttccagtacc tgagcagcag
     6721 cgacagcaag atcagctacg aaaccttcaa gaagcacatc ctgaatctgg ccaagggcaa
     6781 gggcagaatc agcaagacca agaaagagta tctgctggaa gaacgggaca tcaacaggtt
     6841 ctccgtgcag aaagacttca tcaaccggaa cctggtggat accagatacg ccaccagagg
     6901 cctgatgaac ctgctgcgga gctacttcag agtgaacaac ctggacgtga aagtgaagtc
     6961 catcaatggc ggcttcacca gctttctgcg gcggaagtgg aagtttaaga aagagcggaa
     7021 caaggggtac aagcaccacg ccgaggacgc cctgatcatt gccaacgccg atttcatctt
     7081 caaagagtgg aagaaactgg acaaggccaa aaaagtgatg gaaaaccaga tgttcgagga
     7141 aaagcaggcc gagagcatgc ccgagatcga aaccgagcag gagtacaaag agatcttcat
     7201 caccccccac cagatcaagc acattaagga cttcaaggac tacaagtaca gccaccgggt
     7261 ggacaagaag cctaatagaa agctgattaa cgacaccctg tactccaccc ggaaggacga
     7321 caagggcaac accctgatcg tgaacaatct gaacggcctg tacgacaagg acaatgacaa
     7381 gctgaaaaag ctgatcaaca agagccccga aaagctgctg atgtaccacc acgaccccca
     7441 gacctaccag aaactgaagc tgattatgga acagtacggc gacgagaaga atcccctgta
     7501 caagtactac gaggaaaccg ggaactacct gaccaagtac tccaaaaagg acaacggccc
     7561 cgtgatcaag aagattaagt attacggcaa caaactgaac gcccatctgg acatcaccga
     7621 cgactacccc aacagcagaa acaaggtcgt gaagctgtcc ctgaagccct acagattcga
     7681 cgtgtacctg gacaatggcg tgtacaagtt cgtgaccgtg aagaatctgg atgtgatcaa
     7741 aaaagaaaac tactacgaag tgaatagcaa gtgctatgag gaagctaaga agctgaagaa
     7801 gatcagcaac caggccgagt ttatcgcctc cttctacaag aacgatctga tcaagatcaa
     7861 cggcgagctg tatagagtga tcggcgtgaa caacgacctg ctgaaccgga tcgaagtgaa
     7921 catgatcgac atcacctacc gcgagtacct ggaaaacatg aacgacaaga ggccccccca
     7981 catcattaag acaatcgcct ccaagaccca gagcattaag aagtacagca cagacattct
     8041 gggcaacctg tatgaagtga aatctaagaa gcaccctcag atcatcaaaa agggctatcc
     8101 ctatgacgtg cccgattatg ccagcctggg cagcggctcc aagcgcaccg ccgatggttc
     8161 cgagttcgaa agccccaaaa aaaagcgcaa ggtctaaaac ttgtttattg cagcttataa
     8221 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8281 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcggcggccc
     8341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     8401 acgtcaatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     8461 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     8521 gtcaatgacg gtaaatggcc cgcctggcat tgtgcccagt acatgacctt atgggacttt
     8581 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc
     8641 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt
     8701 attttttaat tattttgtgc agcgatgggg gcgggggggg gg
//
