LOCUS       sequence-33149-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Basu S, Mehreja R, Thiberge S, Chen MT, Weiss R
  TITLE     Spatiotemporal control of gene expression with pulse-generating 
            networks.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Apr 27. 101(17):6355-60.
  PUBMED    15096621
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Apr 27. 101(17):6355-60."
COMMENT     Sequence Label: pPSSUB-101
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             17..28
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(160..178)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        244..346
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(413..432)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ctttacgcat gcttaatttc tcctctttta tcaccgccag atgtattcga ctataacaaa
       61 ccattttctt gcgtaaacct gtacgatcct acaggtgctt atgttaagta attgtattcc
      121 cagcgataca atagtgtgac aagagatgaa gacgaaaggg cctcgtgata cgcctatttt
      181 tataggttaa tgtcatgata ataatggttt cttagacgtc gatatctggc gaaaatgaga
      241 cgttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg
      301 cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat
      361 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt
      421 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt
      481 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg
      541 cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg
      601 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct
      661 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc
      721 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt
      781 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa
      841 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat
      901 gccgctggcg atccaggttc atcatgccgt ttgtgatggc ttccatgt
//
