LOCUS       sequence-331502-       12726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant 
            N127W and Citrus limon limonene synthase LIS1 under the control of 
            GAL1/GAL2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12726)
  AUTHORS   Peng B, Nielsen LK, Kampranis SC, Vickers CE
  TITLE     Engineered protein degradation of farnesyl pyrophosphate synthase is
            an effective regulatory mechanism to increase monoterpene production
            in Saccharomyces cerevisiae.
  JOURNAL   Metab Eng. 2018 Feb 19. pii: S1096-7176(17)30214-8. doi: 
            10.1016/j.ymben.2018.02.005.
  PUBMED    29471044
REFERENCE   2  (bases 1 to 12726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2018 Feb 19. pii: S1096-7176(17)30214-8. doi: 
            10.1016/j.ymben.2018.02.005."
COMMENT     Sequence Label: pJT11
FEATURES             Location/Qualifiers
     source          1..12726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..442
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    1..118
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     413..436
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     complement(1854..1873)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1855..1873)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1883..1900)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1883..1899)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1892..1914)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2040..2495
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2127..2146)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2337..2358
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2796..3203
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             3204..4298
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(4792..4811)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4911..4933
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4971..4989)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      5030..6372
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    5362..5409
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        6399..6503
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6504..6572,6573..7364)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6722..6741)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7535..8123
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8024..8043
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8277..8294
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8411..8432
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8447..8452,8453..8470,8471..8477)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8485..8501
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8490..8512
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8509..8525
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8509..8525
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8544..8564
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8546..8564
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 cggattagaa gccgccgagc gggcgacagc cctccgacgg aagactctcc tccgtgcgtc
       61 ctggtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa
      121 caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc
      181 tggccccaca aaccttcaaa tcaacgaatc aaattaacaa ccataggata ataatgcgat
      241 tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatctatt
      301 aacagatata taaatgcaaa agctgcataa ccactttaac taatactttc aacattttcg
      361 gtttgtatta cttcttattc aaatgtcata aaagtatcaa caaaaaattg ttaatatacc
      421 tctatacttt aacgtcaagg agaaaaaact atagctagca aaatggcttc agaaaaagaa
      481 attaggagag agagattctt gaacgttttc cctaaattag tagaggaatt gaacgcatcg
      541 cttttggctt acggtatgcc taaggaagca tgtgactggt atgcccactc attgaactac
      601 aacactccag gcggtaagct aaatagaggt ttgtccgttg tggacacgta tgctattctc
      661 tccaacaaga ccgttgaaca attggggcaa gaagaatacg aaaaggttgc cattctaggt
      721 tggtgcattg agttgttgca ggcttacttc ttggtcgccg atgatatgat ggacaagtcc
      781 attaccagaa gaggccaacc atgttggtac aaggttcctg aagttgggga aattgccatc
      841 tgggacgcat tcatgttaga ggctgctatc tacaagcttt tgaaatctca cttcagaaac
      901 gaaaaatact acatagatat caccgaattg ttccatgagg tcaccttcca aaccgaattg
      961 ggccaattga tggacttaat cactgcacct gaagacaaag tcgacttgag taagttctcc
     1021 ctaaagaagc actccttcat agttactttc aagactgctt actattcttt ctacttgcct
     1081 gtcgcattgg ccatgtacgt tgccggtatc acggatgaaa aggatttgaa acaagccaga
     1141 gatgtcttga ttccattggg tgaatacttc caaattcaag atgactactt agactgcttc
     1201 ggtaccccag aacagatcgg taagatcggt acagatatcc aagataacaa atgttcttgg
     1261 gtaatcaaca aggcattaga acttgcttcc gcagaacaaa gaaagacttt agacgaaaat
     1321 tacggtaaga aggactcagt cgcagaagcc aaatgcaaaa agattttcaa tgacttgaaa
     1381 attgaacagc tataccacga atatgaagag tctattgcca aggatttgaa ggccaaaatt
     1441 tctcaggtcg atgagtctcg tggcttcaaa gctgatgtct taactgcgtt cttgaacaaa
     1501 gtttacaaga gaagcaaata atagtgacct gcaggaagtt ttgttagaaa ataaatcatt
     1561 ttttaattga gcattcttat tcctatttta tttaaatagt tttatgtatt gttagctaca
     1621 tacaacagtt taaatcaaat tttctttttc ccaagtccaa aatggaggtt tattttgatg
     1681 acccgcatgc gattatgttt tgaaagtata agactacata catgtacata tatttaaaca
     1741 tgtaaacccg tccattatat tgcttacttt cttctttttt gccgttttga cttggacctc
     1801 tggtttgcta tttccttaca atctttgcta caatgggccc ggtacccaat tcgccctata
     1861 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     1921 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     1981 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga
     2041 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     2101 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     2161 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     2221 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     2281 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     2341 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     2401 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     2461 cgcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg
     2521 catctgtgcg gtatttcaca ccgcatatcg accggtcgag gagaacttct agtatatcta
     2581 catacctaat attattgcct tattaaaaat ggaatcccaa caattacatc aaaatccaca
     2641 ttctcttcaa aatcaattgt cctgtacttc cttgttcatg tgtgttcaaa aacgttatat
     2701 ttataggata attatactct atttctcaac aagtaattgg ttgtttggcc gagcggtcta
     2761 aggcgcctga ttcaagaaat atcttgaccg cagttaactg tgggaatact caggtatcgt
     2821 aagatgcaag agttcgaatc tcttagcaac cattattttt ttcctcaaca taacgagaac
     2881 acacaggggc gctatcgcac agaatcaaat tcgatgactg gaaatttttt gttaatttca
     2941 gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga
     3001 gagcgccgga accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat
     3061 cacaatactt gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt
     3121 agcaatcgtc ttactttcta acttttctta ccttttacat ttcagcaata tatatatata
     3181 tatttcaagg atataccatt ctaatgtctg cccctaagaa gatcgtcgtt ttgccaggtg
     3241 accacgttgg tcaagaaatc acagccgaag ccattaaggt tcttaaagct atttctgatg
     3301 ttcgttccaa tgtcaagttc gatttcgaaa atcatttaat tggtggtgct gctatcgatg
     3361 ctacaggtgt tccacttcca gatgaggcgc tggaagcctc caagaaggct gatgccgttt
     3421 tgttaggtgc tgtgggtggt cctaaatggg gtaccggtag tgttagacct gaacaaggtt
     3481 tactaaaaat ccgtaaagaa cttcaattgt acgccaactt aagaccatgt aactttgcat
     3541 ccgactctct tttagactta tctccaatca agccacaatt tgctaaaggt actgacttcg
     3601 ttgttgtcag agaattagtg ggaggtattt actttggtaa gagaaaggaa gacgatggtg
     3661 atggtgtcgc ttgggatagt gaacaataca ccgttccaga agtgcaaaga atcacaagaa
     3721 tggccgcttt catggcccta caacatgagc caccattgcc tatttggtcc ttggataaag
     3781 ctaatgtttt ggcctcttca agattatgga gaaaaactgt ggaggaaacc atcaagaacg
     3841 aattccctac attgaaggtt caacatcaat tgattgattc tgccgccatg atcctagtta
     3901 agaacccaac ccacctaaat ggtattataa tcaccagcaa catgtttggt gatatcatct
     3961 ccgatgaagc ctccgttatc ccaggttcct tgggtttgtt gccatctgcg tccttggcct
     4021 ctttgccaga caagaacacc gcatttggtt tgtacgaacc atgccacggt tctgctccag
     4081 atttgccaaa gaataaggtc aaccctatcg ccactatctt gtctgctgca atgatgttga
     4141 aattgtcatt gaacttgcct gaagaaggta aggccattga agatgcagtt aaaaaggttt
     4201 tggatgcagg tatcagaact ggtgatttag gtggttccaa cagtaccacc gaagtcggtg
     4261 atgctgtcgc cgaagaagtt aagaaaatcc ttgcttaaaa agattctctt tttttatgat
     4321 atttgtacat aaactttata aatgaaattc ataatagaaa cgacacgaaa ttacaaaatg
     4381 gaatatgttc atagggtaga cgaaactata tacgcaatct acatacattt atcaagaagg
     4441 agaaaaagga ggatgtaaag gaatacaggt aagcaaattg atactaatgg ctcaacgtga
     4501 taaggaaaaa gaattgcact ttaacattaa tattgacaag gaggagggca ccacacaaaa
     4561 agttaggtgt aacagaaaat catgaaacta tgattcctaa tttatatatt ggaggatttt
     4621 ctctaaaaaa aaaaaaatac aacaaataaa aaacactcaa tgacctgacc atttgatgga
     4681 gtttaagtca ataccttctt gaaccatttc ccataatggt gaaagttccc tcaagaattt
     4741 tactctgtca gaaacggcct taacgacgta gtcgatatgg tgcactctca gtacaatctg
     4801 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     4861 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     4921 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     4981 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagtatg atccaatatc
     5041 aaaggaaatg atagcattga aggatgagac taatccaatt gaggagtggc agcatataga
     5101 acagctaaag ggtagtgctg aaggaagcat acgatacccc gcatggaatg ggataatatc
     5161 acaggaggta ctagactacc tttcatccta cataaataga cgcatataag tacgcattta
     5221 agcataaaca cgcactatgc cgttcttctc atgtatatat atatacaggc aacacgcaga
     5281 tataggtgcg acgtgaacag tgagctgtat gtgcgcagct cgcgttgcat tttcggaagc
     5341 gctcgttttc ggaaacgctt tgaagttcct attccgaagt tcctattctc tagaaagtat
     5401 aggaacttca gagcgctttt gaaaaccaaa agcgctctga agacgcactt tcaaaaaacc
     5461 aaaaacgcac cggactgtaa cgagctacta aaatattgcg aataccgctt ccacaaacat
     5521 tgctcaaaag tatctctttg ctatatatct ctgtgctata tccctatata acctacccat
     5581 ccacctttcg ctccttgaac ttgcatctaa actcgacctc tacatttttt atgtttatct
     5641 ctagtattac tctttagaca aaaaaattgt agtaagaact attcatagag tgaatcgaaa
     5701 acaatacgaa aatgtaaaca tttcctatac gtagtatata gagacaaaat agaagaaacc
     5761 gttcataatt ttctgaccaa tgaagaatca tcaacgctat cactttctgt tcacaaagta
     5821 tgcgcaatcc acatcggtat agaatataat cggggatgcc tttatcttga aaaaatgcac
     5881 ccgcagcttc gctagtaatc agtaaacgcg ggaagtggag tcaggctttt tttatggaag
     5941 agaaaataga caccaaagta gccttcttct aaccttaacg gacctacagt gcaaaaagtt
     6001 atcaagagac tgcattatag agcgcacaaa ggagaaaaaa agtaatctaa gatgctttgt
     6061 tagaaaaata gcgctctcgg gatgcatttt tgtagaacaa aaaagaagta tagattcttt
     6121 gttggtaaaa tagcgctctc gcgttgcatt tctgttctgt aaaaatgcag ctcagattct
     6181 ttgtttgaaa aattagcgct ctcgcgttgc atttttgttt tacaaaaatg aagcacagat
     6241 tcttcgttgg taaaatagcg ctttcgcgtt gcatttctgt tctgtaaaaa tgcagctcag
     6301 attctttgtt tgaaaaatta gcgctctcgc gttgcatttt tgttctacaa aatgaagcac
     6361 agatgcttcg ttcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     6421 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6481 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6541 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     6601 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     6661 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     6721 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     6781 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     6841 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     6901 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     6961 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     7021 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7081 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7141 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7201 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7261 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7321 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     7381 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     7441 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7501 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7561 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7621 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7681 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7741 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7801 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7861 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7921 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7981 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8041 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8101 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8161 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8221 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8281 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     8341 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     8401 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     8461 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     8521 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     8581 tccaccgcgg acgccacaga aacctcagaa taagctaaag ggtaatataa taaagccctt
     8641 cgattcaata atagcattac ccaagaggca tagcggcaaa ctaagttgac aacacaaaat
     8701 caaatcgctt catttttatt ttattaatga tattttatta gagaggaaat tatgtaatat
     8761 ctagatatta aatataaagt agatctctgt ctaaaatatt cttaacaaaa tcttatattc
     8821 gaaaggatga cggtccaatc aaaggattgt ataagagaaa ttagttttcg aaatcaattt
     8881 ttacttgacc tgaacttaac gatttgctct caatccgcca tggtcactat taaccctttg
     8941 tacctggtga tgcggtgaat gccatgtgtt tatcttctaa tggaataggt tggaataaca
     9001 aagtaaaacc gacatcaatg gtttcttgat tttgtacacc gtgaccgtca ccatgcaaat
     9061 acatgaaatg agacattcta actaagttca acaagaattc ggttgtagta ccagtcaatg
     9121 gggagtcttt atctgcggtg taagcattaa cctttttcca catttgtctc atcatatcct
     9181 taatgtgttg tctggcaact tcttctgatg cacctgtttc atgcatgtag cattgtatag
     9241 atttaggtac gtcacctctt tgaatttcgt cactggaagt acctaagtca tcttgcaatc
     9301 tgaaaatttt tgaagaccag tgaacgatat ctgggtttga ttccaagaat tccaattcct
     9361 ttttaatgat agggttagta ccactcaaat aggagattgt gatgatcaat ggaccagtga
     9421 ttgagactaa accgttttcc aagtattctt ccaacttagg tgtgtactta gaatggtacc
     9481 acttagcctc taccaaatag gcttgtatca aacccaacca agcgttttta atagataaca
     9541 ataagtcgaa atcttgttgc ttcaaaacgt agtaagcgaa ttcgttgacg aagttgtaca
     9601 aggctaaaaa gcacatcttc atgtaacctg gcaaatgctt taatgcatag ttgatatccc
     9661 atctttcaac agcgtctgta aagatttcca attcatccaa agtaccgtat acgtcataga
     9721 tgtcatctat aacggtgatc aatgcaatag agattgtcaa aactcttcta cagtaagcga
     9781 attgaggttc aaaggcaata cccattgacc ataagaaaga tgcgaccaat ctatttctag
     9841 cgaatgacaa cttttcaccc aaaccggtat ccttccacca accagagatt tccttcaatt
     9901 cttcttgata gatggcttgt aatgtattga attccatctt tgccaattcc aacaacaagt
     9961 ggttcttatc ttctcttctt tcgtatatat ggatgaacca tcttgcttcc aacataggaa
    10021 ctttccaatg caatggtaat tccaaggctc tctttgcttg ttcagctacg aaaacatctt
    10081 cttccatatt tttacttatc ataacttcct tcaagtgttt ggaagtgaat tgccatgctt
    10141 cttccattat actttcacct tccaatgagt agtaagaagc ttcatgcaag gacaatatac
    10201 ccttgaagtc atcacaaata aaaccacctt ggtcatcctt gaaaccgtta aaaacttctt
    10261 gactgactgg gtaaccgtgt tgtcttaaca atctgaattc caaggaagta gcgtacaaat
    10321 tttcctttct ccagttgtaa tccttattgt tgttatagat gttgttcaag atgtttctga
    10381 tttcggtttc aaatctatgg gctaaaccca atctttgcaa gttatcaatc aattccaatt
    10441 ggtccaaagg ttcgatgaca tctttgatgg ctatctttac tttaccacgt aattcttctg
    10501 ctcttctctt gtatgcttcg tcggtgtagt tagagttcaa ggattgtaaa aagtcgtggt
    10561 cccaaatgga tggttgatag ttagcggatc ttctcatttt agatcctatg aaagaattat
    10621 tttttttatt atgttaatct tgtgtttact taactattgc tattcttgat gataattgaa
    10681 taaggtgcat aatgaagagc aattcacaac accaaatttt caatccaatt actgattgtt
    10741 tatatatgtc tacaaaacta atcctatctc cacattttag cctgcgaaat gtttgttttt
    10801 taaacaatag ctctccagaa cattgtataa tttaagaata tgtgcacagt taactttcta
    10861 gcaggagtat aatgccattt gctccccatc ttgagatggg aagggcttaa ctaatctcgg
    10921 ttcggagtga tccgccccga tactgccttc tgccttaata tcgtccaagg cacatggacc
    10981 cctgaacggc gcagatatct ccgcacggac gaaagaccgc cggtgccttc ctgaggcaac
    11041 cgcccctttc gaatatagat cacgtgaccc atttttagct actaatagaa aaagaaattg
    11101 caacctactt aagccattcc ggaaggaagc tttccgaatt gacgttgaat accttttctg
    11161 aaaagattct accgcattta attggagttt aatgcaaagt agttgccagt tacaagttga
    11221 gtgcggctac ctgatgtttt ccagtcctat attgctgtga tctgaatctg gaaagttcct
    11281 ctggtccgta aacctccttg gattagtaca ctcgaggtta gttttctttg gatatagcag
    11341 ttgttgtaca ctagcttaag atgtaaaatt caattcaata tgattttgtg tgggatgttt
    11401 taaagaaggc acaagcctgt tctctctttt tctctttttt catggattgt atcgcttaaa
    11461 agggagaaat gaagtaaacg attctttttg taaaaatata aaaccaaatt ttttaattta
    11521 tatgtataca tatttattta tgacgtaaga ccaagtaagt ttatatgaaa gtattttgtg
    11581 tatatgacgg aaagaaatgc aggttggtac attacaactt aattctgaca gcttttactt
    11641 cagtgtatgc atggtagact tcttcaccca tttctctacc gtaaccagat tgcttaacac
    11701 caccgaatgg aactctggag tcaaaatcgt tgtatgtgtt gatccagacg gtaccggcct
    11761 tcaacatctt ggccaccttc aaacctgtgc tcaaagattc tgtttcgata ccagaaccta
    11821 gaccgaattc agagctgtta gccatttcga caccttcttc taaagtcttg aactttgcga
    11881 cagtgacaac tggtccaaaa atttcttcct taacaattct catgtcttca ttaacatcgt
    11941 agaaaacggt tggtctgatg aagtaaccct tgtcaccaac tttttcgcca ccagttaaga
    12001 tcttggcgcc ttctttctta ccgatatcga tgtagttcat aattgtgtcg aattgttgac
    12061 ggttagtgat agcaccttgg aagttagcct tgtcaaatgg attaccaact ttgatttcgg
    12121 tttccaagta agccttgaaa gcagccaata gttcgtcgta aataccttct tgaacgtaaa
    12181 ttctagaacc agaggaacaa atttgaccag cgttcttgaa aataccgttt actagatttg
    12241 gtaaagtctt cttaatgtta gcatcgtcaa agaccaaatg ggcggactta ccacctagtt
    12301 ccaaagtgat tttcttcaag ttagattcag aagagtcgac agcaacactc ttaccgactt
    12361 ctgtagaacc ggtaaaagcc agctttctga ttcttgggtc gttggtcaaa gcagcaccaa
    12421 cagttctacc aggacctgga acgatgttga cgacaccagc tggaatacca accttcttac
    12481 ataaagaagc aaagtatagg gctttactag tttatattga attttcaaaa attcttactt
    12541 tttttttgga tggacgcaaa gaagtttaat aatcatatta catggcaata ccaccatata
    12601 catatccata tctaatctta cttatatgtt gtggaaatgt aaagagcccc attatcttag
    12661 cctaaaaaaa ccttctcttt ggaactttca gtaatacgct taactgctca ttgctatatt
    12721 gaagta
//
