LOCUS       sequence-331503-        8494 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish expression vector for heat-shock inducible expression of 
            APEX2-GBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 8494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: HSP70l-APEX2-GBP in pDEST-Tol2-pA2-acrys-mCherry
FEATURES             Location/Qualifiers
     source          1..8494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(638..649,650..812)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    1181..1302
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1425..2135)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEDDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGHHSTGGMDELYK"
     primer_bind     complement(1505..1528)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(1703..1722)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1972..1990
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2132..2141
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2783..2799
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2819..2839
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    4359..4383
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4384..4393
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4390..5139
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="MGKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAG
                     TFDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVA
                     VEVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGH
                     TIGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVD
                     KYAADEDAFFADYAEAHQKLSELGFADA"
     protein_bind    complement(5141..5165)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5170..5511
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid 
                     antibody (Rothbauer et al., 2008)"
                     /label=GFP nanobody
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
     protein_bind    complement(5515..5535)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(5543..5559)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5575..5709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(6148..6335,6336..6347)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6381..6397)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6381..6397)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6434..6454)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6434..6452)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6473..6489)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6486..6508)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6497..6513
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6521..6527,6528..6545,6546..6551))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6566..6587
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6704..6721)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6875..7463)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6955..6974)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7634..8425,8426..8494))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8257..8276
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc acgctggcta
      361 agaactcatc agcctccccg gtccatctac ccacgtacca atgcaccaat tggccacaat
      421 gacggctact acatggtgcc attccttcct ctttatagga atggagacta cctcctgtcc
      481 aacaatgctc ttggatacga gtacgcctac ctgttggacc caggtcattg cacaacacca
      541 gaaatgccct ctgatctgca aaagacgtga atatctgttc agacacccat atccactctg
      601 ttccacacag gtcagaggtt tgtccaggag ttcttgacag aggtgtaaaa agtactcaaa
      661 aattttactc aagtgaaagt acaagtactt agggaaaatt ttactcaatt aaaagtaaaa
      721 gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt actccattaa aattgtactt
      781 gagtattaag gaagtaaaag taaaagcaag aaagaaaact agagattctt gtttaagctt
      841 ttaatctcaa aaaacattaa atgaaatgca tacaaggttt tatcctgctt tagaactgtt
      901 tgtatttaat tatcaaacta taagacagac aatctaatgc cagtacacgc tactcaaagt
      961 tgtaaaacct cagatttaac ttcagtagaa gctgattctc aaaattgtta gtgtcaagcc
     1021 tagctctttt ggggctgaaa agcaatcctg cagtgctgaa aagcctctca caggcagccg
     1081 atgcgggaag aggtgtatta gtcttgatag agaggctgca aatagcagga aacgtgagca
     1141 gagactccct ggtgtctgaa acacaggccc atttacgcct taagatacat tgatgagttt
     1201 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     1261 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     1321 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     1381 tacaaatgtg gtatggctga ttatgatcta gagtcgcggc cgctttactt gtacagctcg
     1441 tccatgccgc cggtggagtg gtggccctcg gcgcgctcgt actgttccac gatggtgtag
     1501 tcctcgttgt gggaggtgat gtccagcttg atgttgacgt tgtaggcgcc gggcagctgc
     1561 acgggcttct tggccttgta ggtggtcttg acctcggcgt cgtagtggcc gccgtccttc
     1621 agcttcagcc tctgcttgat ctcgcccttc agggcgccgt cctcggggta catccgctcg
     1681 gaggaggcct cccagcccat ggtcttcttc tgcattacgg ggccgtcgga ggggaagttg
     1741 gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct gcagggagga gtcctgggtc
     1801 acggtcacca cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg
     1861 aaggacagct tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc cttggagccg
     1921 tacatgaact gaggggacag gatgtcccag gcgaagggca gggggccgcc cttggtcacc
     1981 ttcagcttgg cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg
     2041 aactcgtggc cgttcacgga gccctccatg tgcaccttga agcgcatgaa ctccttgatg
     2101 atggccatgt tgtcatcctc gcccttgctc accatggtgg cgaccggtaa tgtcagacct
     2161 ggtaactcct tactgtaact tattgcacct gatgaacaaa agatgtgaac tgaacgcaag
     2221 taggaggcta atagaggcta gtacagtaca aagatcccca aatgatgtgt gccggcctta
     2281 gtggaggcag acccctggtt atatacctgt gctgcagaaa gaggggctta caaatggatc
     2341 tctctggaat ggcattatct catggtggga acagtgttgt cagcagaatc agcggggtgg
     2401 actctgacct gagaatgaca gtctagcact gaatggctca gacaatactc ttgtggccaa
     2461 ctatcaccgg aaaataagtg cctgccaaaa caaaccagac cagatgaagc tacatggctg
     2521 tagaacgtca gtatttaatg tgttgtggcc acttggttca ccagatttaa gagctaattt
     2581 ttttaaatat cacagataat caatcgcttt tttttttctt gactgaaaag cttagactgg
     2641 aaaattcttt gaaaaagttt gcactacaga aaataaatac ataacatcct gcactgaatg
     2701 cacactatta atagaaaaga gagcattatt gtaggaaatg tattgtatat gtacattctg
     2761 tgcatttcac aattttctcg agcaggaaac agctatgacc atgattacgc caagctatca
     2821 actttgtata gaaaagttgc ttcaggggtg tcgcttggtt atttccaaaa atcaaattaa
     2881 ttttattaaa ctattagaac gagcatgttt tgtctatatg ctacagaaga taaaaaataa
     2941 taggagttaa cagttataaa acaacacact ttgtttctat tgattgttga ccacactggg
     3001 gtctcattaa gttagattaa agacacacta actgggtcaa aagcagcaga ttgatttcat
     3061 agcaccaggg taaactttct aacactttta cggcaatcat atacattaaa attaaataca
     3121 gaccacgact gaacaaggag gatgatctcc aatattaaac aaagagactt gtgcctattt
     3181 ctctgagggt aaacatgacc tctcaagtta gcaagttggt tttaacacta caaaaatagt
     3241 taagactgca atcccagaat aaagtattgg ttttaaccaa tcaatatagt acagtaaaca
     3301 tccatttgtt ttgttgaaac gttaaacaaa tctgaccaaa gctattagct tatataaaac
     3361 aggtttgcct tctatgtagc tgaaaacacc acaggcccga ttttgctact gtgtaaaaca
     3421 tttcagcaag atttttttat tgcatttttt tttactgaat cgttcaaaca ttttatcatt
     3481 ttagtttgtt cattcattgc aactggaaaa acaacacatc acacaaccgc acatatttca
     3541 gcaataagta caataaaaca ctcaaataaa aaaaacattt taaatctctt tgtatttttg
     3601 accgctgttt cgcgtaattt cacggtaaaa ctctggaaat ctccactaca ttcctctcag
     3661 cggctcctct caatgacagc tgaagaagtg acgcggctgc ctgctgtgtt ttgattggtc
     3721 gaattcactg gaggcttcca gaacagtgta gagtctgaac gggtgcgcgc tctgctgtat
     3781 ttaaagggcg aaagagagac cgcagagaaa ctcaaccgaa gagaagcgac ttgacaaaga
     3841 agaaaagagc agcctgacag gactttttcc ccgacgaggt gtttattcgc tctatttaag
     3901 aatctactgt aaggtaagtc tcaatatatt gtactctatt ggctaatcag aattatatag
     3961 agattatatg tacttaatgt caaaaaatca actttgtata tgtaatcttt ttacatgtgg
     4021 actgcctatg ttcatcttat tttaggtcta ctagaaaatt atatttcccg ttttcacaat
     4081 aaggattttt aaaaaaagca atgaacagac gggcatttac tttatgttgc tgacattatt
     4141 ttatatgagc ataataacca taaatactag caaatgtcct aaatgaattt gtgttaatgt
     4201 tgtctacaaa agaaaattag cgttttactt gtacaactaa taataacttg gttattaaga
     4261 gaatttcact tgttgactag aaaaatcctt tcataatgaa acaattgcac cataaattgt
     4321 ataaatataa aattaattct aattgttttt ttttcctgcc aagtttgtac aaaaaagcag
     4381 gctgccacca tgggaaagtc ttacccaact gtgagtgctg attaccagga cgccgttgag
     4441 aaggcgaaga agaagctcag aggcttcatc gctgagaaga gatgcgctcc tctaatgctc
     4501 cgtttggcat tccactctgc tggaaccttt gacaagggca cgaagaccgg tggacccttc
     4561 ggaaccatca agcaccctgc cgaactggct cacagcgcta acaacggtct tgacatcgct
     4621 gttaggcttt tggagccact caaggcggag ttccctattt tgagctacgc cgatttctac
     4681 cagttggctg gcgttgttgc cgttgaggtc acgggtggac ctaaggttcc attccaccct
     4741 ggaagagagg acaagcctga gccaccacca gagggtcgct tgcccgatcc cactaagggt
     4801 tctgaccatt tgagagatgt gtttggcaaa gctatggggc ttactgacca agatatcgtt
     4861 gctctatctg ggggtcacac tattggagct gcacacaagg agcgttctgg atttgagggt
     4921 ccctggacct ctaatcctct tattttcgac aactcatact tcacggagtt gttgagtggt
     4981 gagaaggaag gtctccttca gctaccttct gacaaggctc ttttgtctga ccctgtattc
     5041 cgccctctcg ttgacaaata tgcagcggac gaagatgcct tctttgctga ttacgctgag
     5101 gctcaccaaa agctttccga gcttgggttt gctgatgccg acccagcttt cttgtacaaa
     5161 gtggtgcagg tgcagctggt ggagtctgga ggagctctgg tgcagcctgg aggaagcctg
     5221 cgcctgagct gtgcagctag cggatttcct gtgaaccgct acagcatgcg ctggtaccgc
     5281 caggctcctg gtaaagagcg cgagtgggtg gctggaatga gcagcgctgg agatcgcagc
     5341 agctacgagg acagcgtgaa aggacgcttt acaatcagcc gcgatgatgc tcgcaacaca
     5401 gtgtacctgc agatgaactc tctgaaacct gaggacactg ctgtgtacta ctgtaacgtg
     5461 aacgtgggtt tcgagtactg gggacaggga acacaggtga cagtgagctc ttgacaactt
     5521 tattatacat agttgataat tcactggccg tcgttttacg gtaccatcga tgatgatcca
     5581 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     5641 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     5701 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     5761 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcctcta
     5821 gatcagatct gcgaagatac ggccacgggt gctcttgatc ctgtggctga ttttggactg
     5881 tgctgctcgc agctgctgat gaatcacata cttcctccat tttcttccac tgattgactg
     5941 ttataatttc cctaatttcc aggtcaaggt gctgtgcatt gtggtaatag atgtgacatg
     6001 acgtcacttc caaaggacca atgaacatgt ctgaccaatt tcatataatg tgaaaacgat
     6061 tttcataggc agaataaata acatttaaat taaactgggc atcagcgcaa ttcaattggt
     6121 ttggtaatag caagggaaaa tagaatgaag tgatctccaa aaaataagta ctttttgact
     6181 gtaaataaaa ttgtaaggag taaaaagtac ttttttttct aaaaaaatgt aattaagtaa
     6241 aagtaaaagt attgattttt aattgtactc aagtaaagta aaaatcccca aaaataatac
     6301 ttaagtacag taatcaagta aaattactca agtactttac acctctggtt cttgaccccc
     6361 taccttcagc aagcccagca gatccactag ttctagagcg gccgccaccg cggtggagct
     6421 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
     6481 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6541 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6601 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6661 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6721 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6781 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6841 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6901 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6961 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7021 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7081 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7141 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7201 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7261 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7321 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7381 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7441 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7501 agtggaacga aaactcacgt taaggcattt tggtcatgag attatcaaaa aggatcttca
     7561 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7621 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7681 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7741 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7801 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7861 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7921 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7981 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8041 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8101 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8161 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8221 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8281 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8341 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8401 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8461 gaataagggc gacacggaaa tgttgaatac tcat
//
