LOCUS       sequence-331504-        9270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9270)
  AUTHORS   Zhao B, Wei X, Li W, Udan RS, Yang Q, Kim J, Xie J, Ikenoue T, Yu J,
            Li L, Zheng P, Ye K, Chinnaiyan A, Halder G, Lai ZC, Guan KL
  TITLE     Inactivation of YAP oncoprotein by the Hippo pathway is involved in 
            cell contact inhibition and tissue growth control.
  JOURNAL   Genes Dev. 2007 Nov 1. 21(21):2747-61.
  PUBMED    17974916
REFERENCE   2  (bases 1 to 9270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2007 Nov 1. 21(21):2747-61."
COMMENT     Sequence Label: pQCXIH-Myc-YAP-5SA
FEATURES             Location/Qualifiers
     source          1..9270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             653..682
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    join(2195..2769,2770..2772,2773..2781)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2362..2379)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2589..2608
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2807..3826
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             2807..3826
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     LTR             4373..4798
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(4901..4923)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5059..5079)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5064..5393
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5244..5379
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5415..5434
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5515..5532)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5686..6274)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5766..5785)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6445..7236,7237..7305))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7068..7087
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7306..7410)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7478..7496
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        7596..7975
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7976..8179
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8180..8355
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    8418..8617
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             8818..9234
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     9164..9186
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     9202..9218
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccg
      601 ggaacggtgc attggaacgg acctgcagga attgatccgc ggccgcacca tggaacaaaa
      661 actcatctca gaagaggatc tggcgggcaa ggatcccggg cagcagccgc cgcctcaacc
      721 ggccccccag ggccaagggc agccgccttc gcagcccccg caggggcagg gcccgccgtc
      781 cggacccggg caaccggcac ccgcggcgac ccaggcggcg ccgcaggcac cccccgccgg
      841 gcatcagatc gtgcacgtcc gcggggacgc ggagaccgac ctggaggcgc tcttcaacgc
      901 cgtcatgaac cccaagacgg ccaacgtgcc ccagaccgtg cccatgaggc tccggaagct
      961 gcccgactcc ttcttcaagc cgccggagcc caaatcccac tcccgacagg ccgctactga
     1021 tgcaggcact gcaggagccc tgactccaca gcatgttcga gctcatgccg ctccagctgc
     1081 tctgcagttg ggagctgttt ctcctgggac actgaccccc actggagtag tctctggccc
     1141 agcagctaca cccacagctc agcatcttcg acaggctgct tttgagatac ctgatgatgt
     1201 acctctgcca gcaggttggg agatggcaaa gacatcttct ggtcagagat acttcttaaa
     1261 tcacatcgat cagacaacaa catggcagga ccccaggaag gccatgctgt cccagatgaa
     1321 cgtcacagcc cccaccagtc caccagtgca gcagaatatg atgaactcgg cttcaggtcc
     1381 tcttcctgat ggatgggaac aagccatgac tcaggatgga gaaatttact atataaacca
     1441 taagaacaag accacctctt ggctagaccc aaggcttgac cctcgttttg ccatgaacca
     1501 gagaatcagt cagagtgctc cagtgaaaca gccaccaccc ctggctcccc agagcccaca
     1561 gggaggcgtc atgggtggca gcaactccaa ccagcagcaa cagatgcgac tgcagcaact
     1621 gcagatggag aaggagaggc tgcggctgaa acagcaagaa ctgcttcggc aggagttagc
     1681 cctgcgtagc cagttaccaa cactggagca ggatggtggg actcaaaatc cagtgtcttc
     1741 tcccgggatg tctcaggaat tgagaacaat gacgaccaat agctcagatc ctttccttaa
     1801 cagtggcacc tatcactctc gagatgaggc tacagacagt ggactaagca tgagcagcta
     1861 cagtgtccct cgaaccccag atgacttcct gaacagtgtg gatgagatgg atacaggtga
     1921 tactatcaac caaagcaccc tgccctcaca gcagaaccgt ttcccagact accttgaagc
     1981 cattcctggg acaaatgtgg accttggaac actggaagga gatggaatga acatagaagg
     2041 agaggagctg atgccaagtc tgcaggaagc tttgagttct gacatcctta atgacatgga
     2101 gtctgttttg gctgccacca agctagataa agaaagcttt cttacatggt tatagttaat
     2161 taacggatcc ggaattccgc cccccccccc cccccccctc tccctccccc ccccctaacg
     2221 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     2281 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     2341 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     2401 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     2461 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     2521 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     2581 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     2641 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     2701 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     2761 gatgataagc ttgccacaac ccgtaccaaa gatggataga tccggaaagc ctgaactcac
     2821 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
     2881 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
     2941 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     3001 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct
     3061 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
     3121 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
     3181 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
     3241 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     3301 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
     3361 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
     3421 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
     3481 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
     3541 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
     3601 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
     3661 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
     3721 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
     3781 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagagta gatgccgacc
     3841 gaacaagagc tgatttcgag aacgcctcag ccagcaactc gcgcgagcct agcaaggcaa
     3901 atgcgagaga acggccttac gcttggtggc acagttctcg tccacagttc gctaagctcg
     3961 ctcggctggg tcgcgggagg gccggtcgca gtgattcagg cccttctgga ttgtgttggt
     4021 ccccagggca cgattgtcat gcccacgcac tcgggtgatc tgactgatcc cgcagattgg
     4081 agatcgccgc ccgtgcctgc cgattgggtg cagatctcta tcgagggctg cagcgctgca
     4141 gaggccgagt gcagaactgc tccaaaggga cctcaaggct ttccgaggga cactaggctg
     4201 actccatcga gccagtgtag agataagctt atcgattagt ccaatttgtt aaagacagga
     4261 tatcagtggt ccaggctcta gttttgactc aacaatatca ccagctgaag cctatagagt
     4321 acgagccata gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
     4381 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa
     4441 atacataact gagaatagag aagttcagat caaggtcagg aacagatgga acagggtcga
     4501 ccctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
     4561 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
     4621 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg
     4681 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
     4741 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg
     4801 ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc accgggaggt
     4861 aagctggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     4921 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     4981 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     5041 gagtgtagat ccggctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     5101 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     5161 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     5221 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     5281 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     5341 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctta
     5401 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     5461 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     5521 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     5581 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     5641 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     5701 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     5761 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     5821 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     5881 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     5941 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6001 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6061 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6121 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6181 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6241 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6301 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6361 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     6421 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     6481 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     6541 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     6601 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     6661 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     6721 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg
     6781 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     6841 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     6901 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     6961 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7021 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc
     7081 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7141 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7201 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     7261 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     7321 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     7381 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     7441 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     7501 tcgtcttcaa gaattagctt ggccattgca tacgttgtat ccatatcata atatgtacat
     7561 ttatattggc tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta
     7621 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     7681 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     7741 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     7801 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     7861 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     7921 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     7981 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     8041 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     8101 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     8161 ggtctatata agcagagctc aataaaagag cccacaaccc ctcactcggc gcgccagtct
     8221 tccgatagac tgcgtcgccc gggtacccgt attcccaata aagcctcttg ctgtttgcat
     8281 ccgaatcgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccacga
     8341 cgggggtctt tcatttgggg gctcgtccgg gatttggaga cccctgccca gggaccaccg
     8401 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
     8461 tctatgtttg atgttatgcg cctgcgtctg tactagttag ctaactagct ctgtatctgg
     8521 cggacccgtg gtggaactga cgagttctga acacccggcc gcaaccctgg gagacgtccc
     8581 agggactttg ggggccgttt ttgtggcccg acctgaggaa gggagtcgat gtggaatccg
     8641 accccgtcag gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg
     8701 tctgaatttt tgctttcggt ttggaaccga agccgcgcgt cttgtctgct gcagcgctgc
     8761 agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt atttgtctga aaattagggc
     8821 cagactgtta ccactccctt aagtttgacc ttaggtcact ggaaagatgt cgagcggatc
     8881 gctcacaacc agtcggtaga tgtcaagaag agacgttggg ttaccttctg ctctgcagaa
     8941 tggccaacct ttaacgtcgg atggccgcga gacggcacct ttaaccgaga cctcatcacc
     9001 caggttaaga tcaaggtctt ttcacctggc ccgcatggac acccagacca ggtcccctac
     9061 atcgtgacct gggaagcctt ggcttttgac ccccctccct gggtcaagcc ctttgtacac
     9121 cctaagcctc cgcctcctct tcctccatcc gccccgtctc tcccccttga acctcctcgt
     9181 tcgaccccgc ctcgatcctc cctttatcca gccctcactc cttctctagg cgccggaatt
     9241 gaagatctgg gggatcgatc ctctagagtc
//
