LOCUS       sequence-33154-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral expression of humanized ChR2 with E123T/H134R 
            mutations fused to EYFP driven by CaMKIIa promoter for optogenetic 
            activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Gunaydin LA, Yizhar O, Berndt A, Sohal VS, Deisseroth K, Hegemann P
  TITLE     Ultrafast optogenetic control.
  JOURNAL   Nat Neurosci. 2010 Mar;13(3):387-92. doi: 10.1038/nn.2495.
  PUBMED    20081849
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn"; 
            journalName: "Nat Neurosci"; date: "2010-03"; volume: "13"; issue: 
            "3"; pages: "387-92"
COMMENT     Sequence Label: pLenti-CaMKIIa-ChETA-EYFP
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccttgctcac catggtggcg gccgctggca cggctccggc ctcggcttcg tcttcgacga
       61 gagtctcgac ctcgatctcc gttccgccga tgttcagttt ggtggttttg cggatatctc
      121 cgtgaatcaa tatgtgctcg tggatcagga cgcgcaggta gtgtcccaac aacccccaac
      181 aatttttact catcagatca ataatcgtgt gacctacggt ggagccatag acgctcagga
      241 cgccaaaacc ttcgggcccc aaaatgaaga gaattgggaa cataccccag ctcacgaaaa
      301 acagccatgc catgccggtc acgacctggc ggcaccgacc ctttggcaca gtatgataac
      361 cctcgatata tgctttggcg gcgtgaaaaa atgtgttcgc gccatagcac aatccaagac
      421 aaaagaagat gactttaaca tagccggttg ccatggcgct ggtagccccc cacacgatag
      481 tcccgatgtc tgagacaagg agtcccatgg ttctcctgct gtagtcgttg ctcaggccgg
      541 tgaggttgct caggcggata aggatgacag gacaagtgag cagccaggtt gcatagcgca
      601 gccactgcac ccggtgtcct gtggcaaggt agagcataga gggattctta aactcaaaaa
      661 agaactcgag aatcacctta accatttcaa tggcgcacac atagatctcc tcccagccgc
      721 atgtagattt ccaggtttgg taggcataga acatcagcag caaaatgctg aatcctgctg
      781 caagccactg caggacattt gacgcggtct gagcgccgtt cgtgccgcga gattcaatcc
      841 atccggcaca gtaacattga tcctcaggga ccaggacgga cccgttcacc accacaggat
      901 tagtaacgaa caaaagttcg cgtccgacgg cagacaaagc gccgcc
//
