LOCUS       sequence-331557-        3735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick - E-Luc Luciferase CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Gonzalez-Grandio E, Demirer GS, Ma W, Brady S, Landry MP
  TITLE     A Ratiometric Dual Color Luciferase Reporter for Fast 
            Characterization of Transcriptional Regulatory Elements in Plants.
  JOURNAL   ACS Synth Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248.
  PUBMED    34520169
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248."
COMMENT     Sequence Label: pUPD2+E-Luc
FEATURES             Location/Qualifiers
     source          1..3735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1494..1502
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      1692..1749
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1773..1790)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1944..2532)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2024..2043)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2715..2809)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2830..3489)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3404..3423
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3651..3669
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3673..3716)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    join(3719..3735,1..5)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tagagcgatg agtggtctcg aatggagaga gagaagaacg tggtgtacgg ccccgagccc
       61 aagcaccctc tgggcaactt caccgccggc gagatgctgt acaacgctct gcacaagcac
      121 tcccacatcc cccaggccat cctggacgtg atgggcaacg agtccctttc ctaccaggag
      181 ttcttcgaca ctactgtgaa gctgggccag agcctccaga actgtggcta caagatgaac
      241 gatgtcgtgt cgatctgtgc agagaacaac aagagattct tcatccccat catctccgcc
      301 tggtacatcg gcatggtggt ggcccctgtg aacgaggact atatcccaga cgagctgtgt
      361 aaagtgaccg gcatctccaa gccgatcctg gtctttacca ctaggaagat cctgcctaag
      421 gttttggagg ttaaagacag aaccaactac ataaagagga tcatcatact ggactctgaa
      481 gagaacctgc tgggctgcga gagcctgcac aacttcatgt ccaggtactc cgacaacaac
      541 ctccaaacat tcaagcctct gcactacgac cctgtggacc aggtagccgc catcctgtgc
      601 tcctccggca caaccggcct gcctaaaggc gtgatgcaga cccacaggaa catctgtgtg
      661 agactcacac acgcatctga ccccagagtg ggtacacaac tcatccccgg cgtatccgtg
      721 ctggcctacc tgccattctt ccacgccttc ggcttcagta tcaacctggg ctatttcatg
      781 gtgggcctga gagtggtgat gctccgaagg tttaaccagg aggtgttcct gaaggccatc
      841 caggactacg aggtgaggag cgtgatcaac gttccctcca caatcctgtt cctgtccaag
      901 agccctctgg tggacaagta cgacctatcc accctggcgg agctgtgctg tggagccgct
      961 cctctggcga aggaggtggc cgagatcgcc gtgaagaggc tgaacctgcc agggatacgg
     1021 tgtggctacg gtctaacaga gtctacctcc gccaacatcc atactctgca caacgagttc
     1081 aagtccggct ccctgggcaa ggtgacacct tacatggccg ccaagatcat cgacaggaac
     1141 accggcgagg ccctgggtcc aaaccaggtg ggcgagctgt gcatctgggg acctatggta
     1201 acaaaaggct atgtgaacaa cccacaggct actaaggagg ccatcgacga cgacggctgg
     1261 ctgcactctg gcgacttcgg ctactacgac gaggacgagt atttctacat cgtggaccgg
     1321 tacaaggagc tgatcaaata caagggctat caggtcgccc ctgtggagct ggaggagatc
     1381 ctccttcagc acccaggcat cagggacgtg gccgtcgtgg gtatccctga catcgaggcc
     1441 ggcgagctgc cagccggctt cgtggtgaag cagcccggcg cccaactcac cgctaaggag
     1501 gtgtacgact tcctggccca gagggtgtct cactccaagt acctgagggg cggcgtaagg
     1561 ttcgtggact ctatccccag gaacgtgaca ggcaagatta gtcgaaaaga gctgagggag
     1621 gccctgatgg agaaggcttc taagctgtaa gctttgagac cactcatcgc gactagtagc
     1681 ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
     1741 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
     1801 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1861 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1921 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
     1981 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2041 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2101 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2161 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2221 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2281 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2341 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2401 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2461 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2521 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2581 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2641 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2701 agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     2761 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
     2821 gatatcaaat tacgccccgc cctgccactc gtcgcagtac tgttgtaatt cattaagcat
     2881 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
     2941 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     3001 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgatacgaa
     3061 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     3121 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagtga
     3181 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
     3241 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
     3301 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
     3361 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
     3421 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
     3481 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
     3541 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag
     3601 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     3661 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga
     3721 attcgcggcc gcttc
//
