LOCUS       sequence-331560-        2547 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick - p35SoTMV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Gonzalez-Grandio E, Demirer GS, Ma W, Brady S, Landry MP
  TITLE     A Ratiometric Dual Color Luciferase Reporter for Fast 
            Characterization of Transcriptional Regulatory Elements in Plants.
  JOURNAL   ACS Synth Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248.
  PUBMED    34520169
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248."
COMMENT     Sequence Label: pUPD2+p35SoTMV
FEATURES             Location/Qualifiers
     source          1..2547
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        25..370
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     313..333
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    390..445
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      504..561
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(585..602)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(756..1344)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(836..855)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1527..1621)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1642..2301)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2216..2235
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2463..2481
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2485..2528)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    join(2531..2547,1..5)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tagagcgatg agtggtctcg ggagtgagac ttttcaacaa agggtaatat cgggaaacct
       61 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg
      121 tggctcctac aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctac
      181 cgacagtggt cccaaagatg gacccccacc cacgaggaac atcgtggaaa aagaagatgt
      241 tccaaccacg tcctcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
      301 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
      361 ggagaggaca ggcttcttga gatccttcaa caattaccaa caacaacaaa caacaaacaa
      421 cattacaatt actatttaca attacagtcg actctagagg ataatgtgag accactcatc
      481 gcgactagta gcggccgctg cagtccggca aaaaagggca aggtgtcacc accctgccct
      541 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
      601 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      661 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      721 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac
      781 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      841 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      901 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      961 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1021 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1081 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1141 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     1201 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1261 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1321 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1381 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1441 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1501 acttggtctg acagctcgag gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
     1561 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     1621 ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcgtcgcagt actgttgtaa
     1681 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc
     1741 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
     1801 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     1861 ggctgatacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     1921 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     1981 actccagagt gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     2041 actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt
     2101 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     2161 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     2221 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
     2281 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
     2341 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc
     2401 gatcaactcg agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     2461 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
     2521 atgatttctg gaattcgcgg ccgcttc
//
