LOCUS       sequence-331561-        2745 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick - pAtUBQ10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Gonzalez-Grandio E, Demirer GS, Ma W, Brady S, Landry MP
  TITLE     A Ratiometric Dual Color Luciferase Reporter for Fast 
            Characterization of Transcriptional Regulatory Elements in Plants.
  JOURNAL   ACS Synth Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248.
  PUBMED    34520169
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248."
COMMENT     Sequence Label: pUPD2+pAtUbq10
FEATURES             Location/Qualifiers
     source          1..2745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      702..759
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(783..800)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(954..1542)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1034..1053)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1725..1819)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1840..2499)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2414..2433
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2661..2679
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2683..2726)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    join(2729..2745,1..5)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tagagcgatg agtggtctcg ggaggtcgac gagtcagtaa taaacggcgt caaagtggtt
       61 gcagccggca cacacgagtc gtgtttatca actcaaagca caaatacttt tcctcaacct
      121 aaaaataagg caattagcca aaaacaactt tgcgtgtaaa caacgctcaa tacacgtgtc
      181 attttattat tagctattgc ttcaccgcct tagctttctc gtgacctagt cgtcctcgtc
      241 ttttcttctt cttcttctat aaaacaatac ccaaagagct cttcttcttc acaattcaga
      301 tttcaatttc tcaaaatctt aaaaactttc tctcaattct ctctaccgtg atcaaggtaa
      361 atttctgtgt tccttattct ctcaaaatct tcgattttgt tttcgttcga tcccaatttc
      421 gtatatgttc tttggtttag attctgttaa tcttagatcg aagatgattt tctgggtttg
      481 atcgttagat atcatcttaa ttctcgatta gggtttcata gatatcatcc gatttgttca
      541 aataatttga gttttgtcga ataattactc ttcgatttgt gatttctatc tagatctggt
      601 gttagtttct agtttgtgcg atcgaatttg tcgattaatc tgagtttttc tgattaacag
      661 aatgtgagac cactcatcgc gactagtagc ggccgctgca gtccggcaaa aaagggcaag
      721 gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct
      781 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      841 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      901 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac
      961 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1021 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1081 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1141 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1201 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1261 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1321 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1381 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1441 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1501 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1561 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1621 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1681 taaagtatat atgagtaaac ttggtctgac agctcgaggc ttggattctc accaataaaa
     1741 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
     1801 ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc
     1861 gtcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
     1921 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
     1981 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
     2041 gaaactcacc cagggattgg ctgatacgaa aaacatattc tcaataaacc ctttagggaa
     2101 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
     2161 gaaatcgtcg tggtattcac tccagagtga tgaaaacgtt tcagtttgct catggaaaac
     2221 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg
     2281 aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
     2341 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     2401 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     2461 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     2521 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     2581 ggaacctctt acgtgcccga tcaactcgag tgccacctga cgtctaagaa accattatta
     2641 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat
     2701 ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttc
//
