LOCUS       sequence-331563-        2352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick - pG10-90.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Gonzalez-Grandio E, Demirer GS, Ma W, Brady S, Landry MP
  TITLE     A Ratiometric Dual Color Luciferase Reporter for Fast 
            Characterization of Transcriptional Regulatory Elements in Plants.
  JOURNAL   ACS Synth Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248.
  PUBMED    34520169
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248."
COMMENT     Sequence Label: pUPD2+pG10-90
FEATURES             Location/Qualifiers
     source          1..2352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     199..219
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        208..254
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      309..366
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(390..407)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(561..1149)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(641..660)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1332..1426)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1447..2106)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2021..2040
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2268..2286
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2290..2333)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    join(2336..2352,1..5)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tagagcgatg agtggtctcg ggagatagtt taaactgaag gcgggaaacg acaatctgat
       61 ccaagctcaa gctaagcttg catgcctgca ggatatcgtg gatccaagct tgccacgtgc
      121 cgccacgtgc cgccacgtgc cgccacgtgc ctctagagga tccatctcca ctgacgtaag
      181 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
      241 tcatttggag aggacacgct gggatccaat gtgagaccac tcatcgcgac tagtagcggc
      301 cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg
      361 aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt
      421 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      481 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      541 aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat
      601 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      661 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      721 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      781 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      841 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      901 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      961 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1021 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1081 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1141 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1201 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     1261 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc
     1321 tcgaggcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
     1381 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
     1441 atcaaattac gccccgccct gccactcgtc gcagtactgt tgtaattcat taagcattct
     1501 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     1561 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     1621 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg atacgaaaaa
     1681 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     1741 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagtgatga
     1801 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     1861 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag
     1921 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     1981 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     2041 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     2101 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
     2161 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca actcgagtgc
     2221 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     2281 cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt
     2341 cgcggccgct tc
//
