LOCUS       sequence-331566-        2474 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick - pNRP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Gonzalez-Grandio E, Demirer GS, Ma W, Brady S, Landry MP
  TITLE     A Ratiometric Dual Color Luciferase Reporter for Fast 
            Characterization of Transcriptional Regulatory Elements in Plants.
  JOURNAL   ACS Synth Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248.
  PUBMED    34520169
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248."
COMMENT     Sequence Label: pUPD2+pNRP
FEATURES             Location/Qualifiers
     source          1..2474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        342..388
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      431..488
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(512..529)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(683..1271)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(763..782)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1454..1548)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1569..2228)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2143..2162
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2390..2408
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2412..2455)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    join(2458..2474,1..5)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tagagcgatg agtggtctcg ggagcgaaaa ttcaaacaag tgacaaaaat aatattacga
       61 cccgtccctt tgtttggttg ccccgttcgt gtatttgctt ggccgttggt gttcccatgc
      121 gccacttagc ctccaaagtc ttctctctaa actccttttt tataccataa tctctgttta
      181 gtttatactt aattgcgctt tatcctgcag taaacacaat ttaaatagtt tcaaataaat
      241 ttagaaagaa taaaacaaat agaaatcaga aggtgtctgt ttcctcctcg caacatacga
      301 tcaaagagaa acaacttgac cctttacatt gctcaagagc tgcaagaccc ttcctctata
      361 taaggaagtt catttcattt ggagaggaaa atgtgagacc actcatcgcg actagtagcg
      421 gccgctgcag tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac
      481 cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg
      541 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      601 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      661 aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa
      721 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      781 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      841 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      901 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      961 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1021 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     1081 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     1141 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1201 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1261 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1321 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1381 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1441 gctcgaggct tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
     1501 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg
     1561 atatcaaatt acgccccgcc ctgccactcg tcgcagtact gttgtaattc attaagcatt
     1621 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     1681 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     1741 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgatacgaaa
     1801 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     1861 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagtgat
     1921 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     1981 accagctcac cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca
     2041 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     2101 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     2161 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     2221 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     2281 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt
     2341 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     2401 cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa
     2461 ttcgcggccg cttc
//
