LOCUS       sequence-331570-        2601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick - NOSt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Gonzalez-Grandio E, Demirer GS, Ma W, Brady S, Landry MP
  TITLE     A Ratiometric Dual Color Luciferase Reporter for Fast 
            Characterization of Transcriptional Regulatory Elements in Plants.
  JOURNAL   ACS Synth Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248.
  PUBMED    34520169
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2021 Sep 14. doi: 10.1021/acssynbio.1c00248."
COMMENT     Sequence Label: pUPD2+NOSt
FEATURES             Location/Qualifiers
     source          1..2601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      44..296
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      558..615
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(639..656)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(810..1398)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(890..909)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1581..1675)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1696..2355)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2270..2289
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      complement(2539..2582)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    join(2585..2601,1..5)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tagagcgatg agtggtctcg gcttggaatg gatcttcgat cccgatcgtt caaacatttg
       61 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgacgatta tcatataatt
      121 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
      181 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
      241 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga
      301 attgccaagc taattcttga agatgaaagg gcctcgtgat acgcctattt ttataggtta
      361 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
      421 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
      481 aaccctgata aatgcttcaa taatgggacc gactcgcgct tgagaccact catcgcgact
      541 agtagcggcc gctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct
      601 ttaaaaccga aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc
      661 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      721 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
      781 aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat
      841 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
      901 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
      961 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1021 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1081 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1141 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1201 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1261 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1321 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1381 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1441 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1501 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1561 tctgacagct cgaggcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt
     1621 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
     1681 gagctcgata tcaaattacg ccccgccctg ccactcgtcg cagtactgtt gtaattcatt
     1741 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
     1801 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     1861 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     1921 tacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     1981 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     2041 gagtgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     2101 ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag
     2161 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     2221 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     2281 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     2341 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
     2401 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa
     2461 ctcgagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     2521 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt
     2581 tctggaattc gcggccgctt c
//
