LOCUS       sequence-33158-e         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral expression of eNpHR 3.0 fused to EYFP driven by 
            human Synapsin I promoter for optogenetic inhibition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Gradinaru V, Zhang F, Ramakrishnan C, Mattis J, Prakash R, Diester 
            I, Goshen I, Thompson KR, Deisseroth K
  TITLE     Molecular and cellular approaches for diversifying and extending 
            optogenetics.
  JOURNAL   Cell. 2010 Apr 2. 141(1):154-65.
  PUBMED    20303157
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 2. 141(1):154-65."
COMMENT     Sequence Label: pLenti-hSyn-eNpHR 3.0-EYFP
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(75..326)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(117..138)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     466..485
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattctca ttacacctcg
       61 ttctcgtagc agaacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
      121 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg
      181 tagctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
      241 tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc
      301 accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg
      361 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
      421 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
      481 tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag
      541 aagtcgtgct gcttcatgtg gtcggggtag cgggcgaagc actgcaggcc gtagccgaag
      601 gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg
      661 gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg
      721 tttacgtcgc cgtccagctc gac
//
