LOCUS       sequence-33161-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of humanized ChR2 with E123T/H134R mutations fused to
            EYFP driven by EF1a promoter for optogenetic activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Gunaydin LA, Yizhar O, Berndt A, Sohal VS, Deisseroth K, Hegemann P
  TITLE     Ultrafast optogenetic control.
  JOURNAL   Nat Neurosci. 2010 Mar;13(3):387-92. doi: 10.1038/nn.2495.
  PUBMED    20081849
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn"; 
            journalName: "Nat Neurosci"; date: "2010-03"; volume: "13"; issue: 
            "3"; pages: "387-92"
COMMENT     Sequence Label: pAAV-Ef1a-DIO ChETA-EYFP
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(40..73)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(124..157)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(172..423)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(214..235)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     563..582
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtgtcgtgag gtaccggatc ctctagagtc gactccggaa taacttcgta taggatactt
       61 tatacgaagt tatgcagaat ggtagctgga ttgtagctgc tattagcaat atgaaacctc
      121 ttaataactt cgtatagcat acattatacg aagttatggc gcgccctatt acttgtacag
      181 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
      241 gcgcttctcg ttggggtctt tgctcagggc ggactggtag ctcaggtagt ggttgtcggg
      301 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
      361 gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt
      421 gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat
      481 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
      541 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
      601 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
      661 ggggtagcgg gcgaagcact gcaggccgta gccgaaggtg gtcacgaggg tgggccaggg
      721 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc
      781 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
      841 ca
//
