LOCUS       sequence-331626-       15461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacteria gene circuit on low copy backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15461)
  AUTHORS   Zhang R, Goetz H, Melendez-Alvarez J, Li J, Ding T, Wang X, Tian XJ
  TITLE     Winner-takes-all resource competition redirects cascading cell fate 
            transitions.
  JOURNAL   Nat Commun. 2021 Feb 8;12(1):853. doi: 10.1038/s41467-021-21125-3.
  PUBMED    33558556
REFERENCE   2  (bases 1 to 15461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-02-8"; volume: "12(1):853. doi"; pages: " 
            10.1038/s41467-021-21125-3"
COMMENT     Sequence Label: pMMB206-CT61
FEATURES             Location/Qualifiers
     source          1..15461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             161..172
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             179..892
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGGTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             179..892
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGGTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(209..237)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     832..852
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             899..931
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     terminator      954..1025
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1041..1068
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             1221..1232
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1239..2117
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     terminator      2139..2210
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2226..2253
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             2331..2342
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      3123..3194
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3210..3237
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             3315..3326
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             3333..4007
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      4063..4134
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4150..4177
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             4255..4266
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4273..5151
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     terminator      5173..5244
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5260..5287
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             5440..5451
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             6037..6069
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     terminator      6117..6188
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6204..6231
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    6238..6258
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(6270..6286)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6279..6301)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6456..6473)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      6626..6712
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6804..6831
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        6850..6941
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7384..7486
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             7487..8146
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7553..7572)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      8478..8872
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            9214..9301
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             10540..11511
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             12022..12861
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             12848..13699
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(13976..15058)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     15020..15039
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(15059..15136)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        join(15366..15371,15372..15387,15388..15394)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    15402..15418
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15407..15429
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15436..15452
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15436..15452
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gaattcgcgg ccgcttctag agacattgat tatttgcacg gcgtcacact ttgctatgcc
       61 atagcaagat agtccataag attagcggat cctacctgac gctttttatc gcaactctct
      121 actgtttctc cataccgttt ttttgggcta gctactagag aaagaggaga aatactagat
      181 gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
      241 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
      301 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt
      361 cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca
      421 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa
      481 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtggta cccttgttaa
      541 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt
      601 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat
      661 caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca
      721 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct
      781 gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct
      841 tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aaaggcctgc
      901 tgcaaacgac gaaaactacg ctttagtagc ttaataatac tagagccagg catcaaataa
      961 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1021 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
     1081 agacattgat tatttgcacg gcgtcacact ttgctatgcc atagcaagat agtccataag
     1141 attagcggat cctacctgac gctttttatc gcaactctct actgtttctc cataccgttt
     1201 ttttgggcta gctactagag aaagaggaga aatactagat ggctgaagcg caaaatgatc
     1261 ccctgctgcc gggatactcg tttaacgccc atctggtggc gggtttaacg ccgattgagg
     1321 ccaatggtta tctcgatttt tttatcgacc gaccgctggg aatgaaaggt tatattctca
     1381 atctcaccat tcgcggtcag ggggtggtga aaaatcaggg acgagaattt gtctgccgac
     1441 cgggtgatat tttgctgttc ccgccaggag agattcatca ctacggtcgt catccggagg
     1501 ctcgcgaatg gtatcaccag tgggtttact ttcgtccgcg cgcctactgg catgaatggc
     1561 ttaactggcc gtcaatattt gccaatacgg gtttctttcg cccggatgaa gcgcaccagc
     1621 cgcatttcag cgacctgttt gggcaaatca ttaacgccgg gcaaggggaa gggcgctatt
     1681 cggagctgct ggcgataaat ctgcttgagc aattgttact gcggcgcatg gaagcgatta
     1741 acgagtcgct ccatccaccg atggataatc gggtacgcga ggcttgtcag tacatcagcg
     1801 atcacctggc agacagcaat tttgatatcg ccagcgtcgc acagcatgtt tgcctgtcgc
     1861 cgtcgcgtct gtcacatctt ttccgccagc agttagggat tagcgtctta agctggcgcg
     1921 aggaccaacg catcagccag gcgaagctgc ttttgagcac tacccggatg cctatcgcca
     1981 ccgtcggtcg caatgttggt tttgacgatc aactctattt ctcgcgagta tttaaaaaat
     2041 gcaccggggc cagcccgagc gagttccgtg ccggttgtga agaaaaagtg aatgatgtag
     2101 ccgtcaagtt gtcataataa actactagag ccaggcatca aataaaacga aaggctcagt
     2161 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     2221 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg
     2281 tacagggtta cgcaagaaaa tggtttgtta tagtcgaata aatactagag aaagaggaga
     2341 aatactagat gaaaaacata aatgccgacg acacatacag aataattaat aaaattaaag
     2401 cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa atggtacatt
     2461 gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct gatatttcaa
     2521 tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat ttaataaaat
     2581 atgatcctat agtagattat tctaactcca atcattcacc aattaattgg aatatatttg
     2641 aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa acagcaggtc
     2701 ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga atacttagtt
     2761 ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg tgtatgaaca
     2821 taccattaat tgttccttct ctagttgata attatcgaaa aataaatata gcaaataata
     2881 aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca tgcgaaggaa
     2941 aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc actttccatt
     3001 taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt tctaaagcaa
     3061 ttttaacagg agcaattgat tgcccatact ttaaaaatta ataaactact agagccaggc
     3121 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     3181 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     3241 atatactaga gacctgtagg atcgtacagg gttacgcaag aaaatggttt gttatagtcg
     3301 aataaatact agagaaagag gagaaatact agatggcttc ctccgaagac gttatcaaag
     3361 agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg
     3421 aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca
     3481 aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca
     3541 aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag
     3601 gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg
     3661 actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc
     3721 cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta
     3781 tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg
     3841 gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc
     3901 cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca
     3961 tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgctgct gcaaacgacg
     4021 aaaactacgc agcagcagtt taataatact agagccaggc atcaaataaa acgaaaggct
     4081 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     4141 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gacctgtagg
     4201 atcgtacagg gttacgcaag aaaatggttt gttatagtcg aataaatact agagaaagag
     4261 gagaaatact agatggctga agcgcaaaat gatcccctgc tgccgggata ctcgtttaac
     4321 gcccatctgg tggcgggttt aacgccgatt gaggccaatg gttatctcga tttttttatc
     4381 gaccgaccgc tgggaatgaa aggttatatt ctcaatctca ccattcgcgg tcagggggtg
     4441 gtgaaaaatc agggacgaga atttgtctgc cgaccgggtg atattttgct gttcccgcca
     4501 ggagagattc atcactacgg tcgtcatccg gaggctcgcg aatggtatca ccagtgggtt
     4561 tactttcgtc cgcgcgccta ctggcatgaa tggcttaact ggccgtcaat atttgccaat
     4621 acgggtttct ttcgcccgga tgaagcgcac cagccgcatt tcagcgacct gtttgggcaa
     4681 atcattaacg ccgggcaagg ggaagggcgc tattcggagc tgctggcgat aaatctgctt
     4741 gagcaattgt tactgcggcg catggaagcg attaacgagt cgctccatcc accgatggat
     4801 aatcgggtac gcgaggcttg tcagtacatc agcgatcacc tggcagacag caattttgat
     4861 atcgccagcg tcgcacagca tgtttgcctg tcgccgtcgc gtctgtcaca tcttttccgc
     4921 cagcagttag ggattagcgt cttaagctgg cgcgaggacc aacgcatcag ccaggcgaag
     4981 ctgcttttga gcactacccg gatgcctatc gccaccgtcg gtcgcaatgt tggttttgac
     5041 gatcaactct atttctcgcg agtatttaaa aaatgcaccg gggccagccc gagcgagttc
     5101 cgtgccggtt gtgaagaaaa agtgaatgat gtagccgtca agttgtcata ataaactact
     5161 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     5221 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     5281 ttctgcgttt atatactaga gacattgatt atttgcacgg cgtcacactt tgctatgcca
     5341 tagcaagata gtccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta
     5401 ctgtttctcc ataccgtttt tttgggctag ctactagaga aagaggagaa atactagatg
     5461 actataatga taaaaaaatc ggattttttg gcaattccat cggaggagta taaaggtatt
     5521 ctaagtcttc gttatcaagt gtttaagcaa agacttgagt gggacttagt tgtagaaaat
     5581 aaccttgaat cagatgagta tgataactca aatgcagaat atatttatgc ttgtgatgat
     5641 actgaaaatg taagtggatg ctggcgttta ttacctacaa caggtgatta tatgctgaaa
     5701 agtgtttttc ctgaattgct tggtcaacag agtgctccca aagatcctaa tatagtcgaa
     5761 ttaagtcgtt ttgctgtagg taaaaatagc tcaaagataa ataactctgc tagtgaaatt
     5821 acaatgaaac tatttgaagc tatatataaa cacgctgtta gtcaaggtat tacagaatat
     5881 gtaacagtaa catcaacagc aatagagcga tttttaaagc gtattaaagt tccttgtcat
     5941 cgtattggag acaaagaaat tcatgtatta ggtgatacta aatcggttgt attgtctatg
     6001 cctattaatg aacagtttaa aaaagcagtc ttaaatgctg caaacgacga aaactacgct
     6061 ttagtagctt aataactctg atagtgctag tgtagatctc tactagagcc aggcatcaaa
     6121 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     6181 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac
     6241 tagtagcggc cgctgcagcc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg
     6301 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     6361 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     6421 gatggctgtt ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc
     6481 agaagcggtc tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac
     6541 cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat
     6601 gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     6661 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg
     6721 agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata
     6781 aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct
     6841 acaaactctt ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6901 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     6961 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     7021 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     7081 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt gatgtccggc
     7141 ggtgcttttg ccgttacgca ccaccccgtc agtagctgaa caggagggac agctgataga
     7201 aacagaagcc actggcctca aaaacaccat catacactaa atcagtaagt tggcagcatc
     7261 acccgacgca ctttgcgccg aataaatacc tgtgacggaa gatcacttcg cagaataaat
     7321 aaatcctggt gtccctgttg ataccgggaa gccctgggcc aacttttggc gaaaatgaga
     7381 cgttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg
     7441 cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat
     7501 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt
     7561 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt
     7621 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg
     7681 cctgatgaat gctcatccgg aattacgtat ggcaatgaaa gacggtgagc tggtgatatg
     7741 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct
     7801 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc
     7861 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt
     7921 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa
     7981 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat
     8041 gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct
     8101 taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag
     8161 ttattgtctt caaattcccg ttgcacatag cccggcaatt cctttccctg ctctgccata
     8221 agcgcagcga atgccgggta atactcgtca acgatctgat agagaagggt ttgctcgggt
     8281 cggtggctct ggtaacgacc agtatcccga tcccggctgg ccgtcctggc cgccacatga
     8341 ggcatgttcc gcgtccttgc aatactgtgt ttacatacag tctatcgctt agcggaaagt
     8401 tcttttaccc tcagccgaaa tgcctgccgt tgctagacat tgccagccag tgcccgtcac
     8461 tcccgtacta actgtcacga acccctgcaa taactgtcac gcccccctgc aataactgtc
     8521 acgaacccct gcaataactg tcacgccccc aaacctgcaa acccagcagg ggcgggggct
     8581 ggcggggtgt tggaaaaatc catccatgat tatctaagaa taatccacta ggcgcggtta
     8641 tcagcgccct tgtggggcgc tgctgccctt gcccaatatg cccggccaga ggccggatag
     8701 ctggtctatt cgctgcgcta ggctacacac cgccccaccg ctgcgcggca gggggaaagg
     8761 cgggcaaagc ccgctaaacc ccacaccaaa ccccgcagaa atacgctgga gcgcttttag
     8821 ccgctttagc ggcctttccc cctacccgaa gggtgggggc gcgtgtgcag ccccgcaggg
     8881 cctgtctcgg tcgatcattc agcccggctc atccttctgg cgtggcggca gaccgaacaa
     8941 ggcgcggtcg tggtcgcgtt caaggtacgc atccattgcc gccatgagcc gatcctccgg
     9001 ccactcgctg ctgttcacct tggccaaaat catggccccc accagcacct tgcgccttgt
     9061 ttcgttcttg cgctcttgct gctgttccct tgcccgcacc cgctgaattt cggcattgat
     9121 tcgcgctcgt tgttcttcga gcttggccag ccgatccgcc gccttgttgc tccccttaac
     9181 catcttgaca ccccattgtt aatgtgctgt ctcgtaggct atcatggagg cacagcggcg
     9241 gcaatcccga ccctactttg taggggaggg cgcacttacc ggtttctctt cgagaaactg
     9301 gcctaacggc cacccttcgg gcggtgcgct ctccgagggc cattgcatgg agccgaaaag
     9361 caaaagcaac agcgaggcag catggcgatt tatcacctta cggcgaaaac cggcagcagg
     9421 tcgggcggcc aatcggccag ggccaaggcc gactacatcc agcgcgaagg caagtatgcc
     9481 cgcgacatgg atgaagtctt gcacgccgaa tccgggcaca tgccggagtt cgtcgagcgg
     9541 cccgccgact actgggatgc tgccgacctg tatgaacgcg ccaatgggcg gctgttcaag
     9601 gaggtcgaat ttgccctgcc ggtcgagctg accctcgacc agcagaaggc gctggcgtcc
     9661 gagttcgccc agcacctgac cggtgccgag cgcctgccgt atacgctggc catccatgcc
     9721 ggtggcggcg agaacccgca ctgccacctg atgatctccg agcggatcaa tgacggcatc
     9781 gagcggcccg ccgctcagtg gttcaagcgg tacaacggca agaccccgga gaagggcggg
     9841 gcacagaaga ccgaagcgct caagcccaag gcatggcttg agcagacccg cgaggcatgg
     9901 gccgaccatg ccaaccgggc attagagcgg gctggccacg acgcccgcat tgaccacaga
     9961 acacttgagg cgcagggcat cgagcgcctg cccggtgttc acctggggcc gaacgtggtg
    10021 gagatggaag gccggggcat ccgcaccgac cgggcagacg tggccctgaa catcgacacc
    10081 gccaacgccc agatcatcga cttacaggaa taccgggagg caatagacca tgaacgcaat
    10141 cgacagagtg aagaaatcca gaggcatcaa cgagttagcg gagcagatcg aaccgctggc
    10201 ccagagcatg gcgacactgg ccgacgaagc ccggcaggtc atgagccaga cccagcaggc
    10261 cagcgaggcg caggcggcgg agtggctgaa agcccagcgc cagacagggg cggcatgggt
    10321 ggagctggcc aaagagttgc gggaggtagc cgccgaggtg agcagcgccg cgcagagcgc
    10381 ccggagcgcg tcgcgggggt ggcactggaa gctatggcta accgtgatgc tggcttccat
    10441 gatgcctacg gtggtgctgc tgatcgcatc gttgctcttg ctcgacctga cgccactgac
    10501 aaccgaggac ggctcgatct ggctgcgctt ggtggcccga tgaagaacga caggactttg
    10561 caggccatag gccgacagct caaggccatg ggctgtgagc gcttcgatat cggcgtcagg
    10621 gacgccacca ccggccagat gatgaaccgg gaatggtcag ccgccgaagt gctccagaac
    10681 acgccatggc tcaagcggat gaatgcccag ggcaatgacg tgtatatcag gcccgccgag
    10741 caggagcggc atggtctggt gctggtggac gacctcagcg agtttgacct ggatgacatg
    10801 aaagccgagg gccgggagcc tgccctggta gtggaaacca gcccgaagaa ctatcaggca
    10861 tgggtcaagg tggccgacgc cgcaggcggt gaacttcggg ggcagattgc ccggacgctg
    10921 gccagcgagt acgacgccga cccggccagc gccgacagcc gccactatgg ccgcttggcg
    10981 ggcttcacca accgcaagga caagcacacc acccgcgccg gttatcagcc gtgggtgctg
    11041 ctgcgtgaat ccaagggcaa gaccgccacc gctggcccgg cgctggtgca gcaggctggc
    11101 cagcagatcg agcaggccca gcggcagcag gagaaggccc gcaggctggc cagcctcgaa
    11161 ctgcccgagc ggcagcttag ccgccaccgg cgcacggcgc tggacgagta ccgcagcgag
    11221 atggccgggc tggtcaagcg cttcggtgat gacctcagca agtgcgactt tatcgccgcg
    11281 cagaagctgg ccagccgggg ccgcagtgcc gaggaaatcg gcaaggccat ggccgaggcc
    11341 agcccagcgc tggcagagcg caagcccggc cacgaagcgg attacatcga gcgcaccgtc
    11401 agcaaggtca tgggtctgcc cagcgtccag cttgcgcggg ccgagctggc acgggcaccg
    11461 gcaccccgcc agcgaggcat ggacaggggc gggccagatt tcagcatgta gtgcttgcgt
    11521 tggtactcac gcctgttata ctatgagtac tcacgcacag aagggggttt tatggaatac
    11581 gaaaaaagcg cttcagggtc ggtctacctg atcaaaagtg acaagggcta ttggttgccc
    11641 ggtggctttg gttatacgtc aaacaaggcc gaggctggcc gcttttcagt cgctgatatg
    11701 gccagcctta accttgacgg ctgcaccttg tccttgttcc gcgaagacaa gcctttcggc
    11761 cccggcaagt ttctcggtga ctgatatgaa agaccaaaag gacaagcaga ccggcgacct
    11821 gctggccagc cctgacgctg tacgccaagc gcgatatgcc gagcgcatga aggccaaagg
    11881 gatgcgtcag cgcaagttct ggctgaccga cgacgaatac gaggcgctgc gcgagtgcct
    11941 ggaagaactc agagcggcgc agggcggggg tagtgacccc gccagcgcct aaccaccaac
    12001 tgcctgcaaa ggaggcaatc aatggctacc cataagccta tcaatattct ggaggcgttc
    12061 gcagcagcgc cgccaccgct ggactacgtt ttgcccaaca tggtggccgg tacggtcggg
    12121 gcgctggtgt cgcccggtgg tgccggtaaa tccatgctgg ccctgcaact ggccgcacag
    12181 attgcaggcg ggccggatct gctggaggtg ggcgaactgc ccaccggccc ggtgatctac
    12241 ctgcccgccg aagacccgcc caccgccatt catcaccgcc tgcacgccct tggggcgcac
    12301 ctcagcgccg aggaacggca agccgtggct gacggcctgc tgatccagcc gctgatcggc
    12361 agcctgccca acatcatggc cccggagtgg ttcgacggcc tcaagcgcgc cgccgagggc
    12421 cgccgcctga tggtgctgga cacgctgcgc cggttccaca tcgaggaaga aaacgccagc
    12481 ggccccatgg cccaggtcat cggtcgcatg gaggccatcg ccgccgatac cgggtgctct
    12541 atcgtgttcc tgcaccatgc cagcaagggc gcggccatga tgggcgcagg cgaccagcag
    12601 caggccagcc ggggcagctc ggtactggtc gataacatcc gctggcagtc ctacctgtcg
    12661 agcatgacca gcgccgaggc cgaggaatgg ggtgtggacg acgaccagcg ccggttcttc
    12721 gtccgcttcg gtgtgagcaa ggccaactat ggcgcaccgt tcgctgatcg gtggttcagg
    12781 cggcatgacg gcggggtgct caagcccgcc gtgctggaga ggcagcgcaa gagcaagggg
    12841 gtgccccgtg gtgaagccta agaacaagca cagcctcagc cacgtccggc acgacccggc
    12901 gcactgtctg gcccccggcc tgttccgtgc cctcaagcgg ggcgagcgca agcgcagcaa
    12961 gctggacgtg acgtatgact acggcgacgg caagcggatc gagttcagcg gcccggagcc
    13021 gctgggcgct gatgatctgc gcatcctgca agggctggtg gccatggctg ggcctaatgg
    13081 cctagtgctt ggcccggaac ccaagaccga aggcggacgg cagctccggc tgttcctgga
    13141 acccaagtgg gaggccgtca ccgctgatgc catggtggtc aaaggtagct atcgggcgct
    13201 ggcaaaggaa atcggggcag aggtcgatag tggtggggcg ctcaagcaca tacaggactg
    13261 catcgagcgc ctttggaagg tatccatcat cgcccagaat ggccgcaagc ggcaggggtt
    13321 tcggctgctg tcggagtacg ccagcgacga ggcggacggg cgcctgtacg tggccctgaa
    13381 ccccttgatc gcgcaggccg tcatgggtgg cggccagcat gtgcgcatca gcatggacga
    13441 ggtgcgggcg ctggacagcg aaaccgcccg cctgctgcac cagcggctgt gtggctggat
    13501 cgaccccggc aaaaccggca aggcttccat agataccttg tgcggctatg tctggccgtc
    13561 agaggccagt ggttcgacca tgcgcaagcg ccgccagcgg gtgcgcgagg cgttgccgga
    13621 gctggtcgcg ctgggctgga cggtaaccga gttcgcggcg ggcaagtacg acatcacccg
    13681 gcccaaggcg gcaggctgac cccccccact ctattgtaaa caagacattt ttatctttta
    13741 tattcaatgg cttattttcc tgctaattgg taataccatg aaaaatacca tgctcagaaa
    13801 aggcttaaca atattttgaa aaattgccta ctgagcgctg ccgcacagct ccataggccg
    13861 ctttcctggc tttgcttcca gatgtatgct cttctgctcc cgaacgccag caagacgtag
    13921 cccagcgcgt cggccagctt gcaattcgcg ctaacttaca ttaattgcgt tgcgctcact
    13981 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    14041 ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg
    14101 gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc
    14161 tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg
    14221 agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg cgcagcccgg
    14281 actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag
    14341 tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc
    14401 agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc
    14461 cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct
    14521 ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa
    14581 taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc
    14641 aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac
    14701 tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt
    14761 ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga
    14821 caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact
    14881 gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg
    14941 cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa
    15001 cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat
    15061 tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc
    15121 accattcgat ggtgtcaacg taaatgccgc ttcgccttcg cgcgcgaatt gcaagctgat
    15181 ccgggcttat cgactgcacg gtgcaccaat gcttctggcg tcaggcagcc atcggaagct
    15241 gtggtatggc tgtgcaggtc gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc
    15301 cgttctggat aatgtttttt gcgccgacat cataacggtt ctggcaaata ttctgaaatg
    15361 agctgttgac aattaatcat cggctcgtat aatgtgtgga attgtgagcg gataacaatt
    15421 tcacacagga aacagcagga aacagctatg accatgatta c
//
