LOCUS       sequence-331712-        8059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Blasticidin resistant 5xQUAS mCherry GMR-White CDS response vector. 
            Contains an Alpha level GB2.0 entry point for addition of custom 
            assemblies utilizing blue-white bacterial colony screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8059)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 8059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pBlastR-5xQUAS-mCherry-GMRWhite
FEATURES             Location/Qualifiers
     source          1..8059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..71
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        125..143
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     125..142
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    150..206
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(208..227)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(209..227)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(234..251)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(234..250)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(243..265)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(459..478)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    535..604
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     1031..1052
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     polyA_signal    2627..2761
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    5295..5429
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(5702..6290)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5782..5801)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             6758..7549
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7604..8032
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7691..7710)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7901..7922
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacggatca gcgagagacc cctgttgaca attaatcatc ggcatagtat atcggcatag
       61 tataatacga caaggtgagg aactaaaccc aggaggcaga tcatgaccat gattacgcca
      121 agctatttag gtgacactat agaatactca agcttgcatg cctgcaggtc gactctagag
      181 gatccccggg taccgagctc gaattcgccc tatagtgagt cgtattacaa ttcactggcc
      241 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
      301 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
      361 caacagttgc gcagcctgaa tggcgaatga cgcctgatgc ggtattttct ccttacgcat
      421 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
      481 tagggtctct ctccggatcc cagggaggaa ttggtcgacg atgtaggtca ctgtctcgaa
      541 gccgcggtgc gggtgccagg gcgtgccctt gggctccccg ggcgcgtact ccacctcacc
      601 catctggtcc atcatgatgc gctgtcagga gctagaatcc caaaacaaac tggttattgt
      661 ggtaggtcat ttgtttggca gaaagaaaac tcgagaaatt tctctggccg ttattcgtta
      721 ttctctcttt tctttttggg tcgctccctc tctgcactaa tgctctctca ctctgtcaca
      781 cagtaaacgg catactgctc tcgttggttc gagagagcgc gcctcgaatg ttcgcgaaaa
      841 gagcgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa ttcaaacaag
      901 caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc tgaacaagct
      961 aaacaatctg cagtaaagtg caagttaaag tgaatcaatt aaaagtaacc agcaaccaag
     1021 taaatcaact gcaactactg aaatctgcca agaagtaatt attgaataca agaagagaac
     1081 actcaatact cgaatccatg tgggagttta ttcttgacac agatatttat gatataataa
     1141 ctgagtaagc ttaacataag gaggaaaaca aaatgaaaac cttcaacatc agccagcagg
     1201 acctggaact ggtggaggtg gctaccgaga agattacgat gctctacgag gataacaagc
     1261 accacgtggg agctgccatt cgcaccaaga cgggcgagat tatcagtgcc gtgcacattg
     1321 aggcctacat cggacgcgtg accgtgtgtg ccgaggccat tgccattggc agtgccgtga
     1381 gcaacggcca gaaggatttt gataccattg tggccgtgcg tcacccatac tccgatgagg
     1441 tggaccgtag catccgtgtc gtgtccccgt gtggaatgtg ccgtgagctg attagcgatt
     1501 acgcccccga ttgcttcgtg ctgattgaga tgaacggcaa gctggtcaag accaccattg
     1561 aggaactgat tcccctgaag tatacccgta actaagcttg gcaataaaaa gacagaataa
     1621 aacgcacggg tgttgggtcg tttgttccgc tgtcaggagg ggtaatcgct tatcctcgga
     1681 taaacaatta tcctcacggg taatcgctta tccgggtaat cgcttatcct cgggtaatcg
     1741 cttatccttt cccgagctcg cccggggatc gagcgcagcg gtataaaagg gcgcggggtg
     1801 gctgagagca tcagttgtga atgaatgttc gagccgagca gacgtgccgc tgccttcgtt
     1861 aatatccttt gaataagcca actttgaatc acaagacgca taccaaacca aatggtgtcc
     1921 aagggagaag aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtccacatg
     1981 gagggcagcg tgaatggcca cgagttcgag atcgagggcg aaggcgaggg acgtccctac
     2041 gaaggcaccc agaccgccaa actgaaggtg accaagggag gcccgttgcc ctttgcctgg
     2101 gacatcttga gcccccagtt catgtacggc tccaaggcct acgtgaagca cccggccgac
     2161 attcccgact acctgaagct gagcttcccc gaaggcttca agtgggagcg cgtgatgaac
     2221 ttcgaggacg gcggagtggt gaccgtgacc caggactcca gcttgcagga cggcgagttc
     2281 atctacaagg tcaagctgcg cggcacgaac tttccctccg atggcccggt gatgcagaag
     2341 aagaccatgg gctgggaggc ttccagcgag cgcatgtatc ccgaggacgg cgctctgaag
     2401 ggcgagatca agcagcgcct gaagctgaag gatggaggcc actacgacgc cgaggtgaag
     2461 accacctaca aggccaagaa gcccgtgcaa ttgcccggcg cctacaacgt gaacatcaag
     2521 ctggacatca ccagccacaa cgaggactac accatcgtgg agcagtacga gcgtgccgaa
     2581 ggacgtcata gcacgggcgg catggatgag ctgtacaagt aagcttgatc cagacatgat
     2641 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     2701 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     2761 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     2821 ttcgctgtca ggagcgtacg actagcacct gactgtcgag cccagtggaa acccttgaaa
     2881 tgcctttaag tcgagcccag tggaaaccct tgaaatgcct ttaagtcgag cccagtggaa
     2941 acccttgaaa tgcctttaag tcgagcccag tggaaaccct tgaaatgcct ttaagtcgag
     3001 cccagtggaa acccttgaaa tgcctttaag tcgagtagca gtcgaggtag gtacgtcatg
     3061 cccctcccga gctcgcccgg ggatcgagcg cagcggtata aaagggcgcg gggtggctga
     3121 gagcatcagt tgtgaatgaa tgttcgagcc gagcagacgt gccgctgcct tcgttaatat
     3181 cctttgaata agccaacttt gaatcacaag acgcatacca aaccaaatgg gacaggagga
     3241 ccaggagctg ctgatccgcg gaggctccaa gcacccaagc gccgagcacc tgaacaacgg
     3301 cgatagcgga gccgccagcc agtcgtgcat caatcaggga tttggccagg ccaagaatta
     3361 cggaaccctg ctgcccccca gccccccaga ggatagcgga agcggatccg gacagctggc
     3421 cgagaatctg acgtacgcct ggcataacat ggatatcttc ggcgccgtga accagccagg
     3481 cagtggctgg cgtcagctgg tgaaccgcac ccgcggactg ttctgcaatg agcgccatat
     3541 cccagcccca cgcaagcacc tgctgaagaa tgtgtgcgga gtggcctacc ccggcgagct
     3601 gctggccgtg atgggaagta gtggcgccgg aaagaccacg ctgctgaacg ccctggcctt
     3661 ccgcagcccc cagggcattc aggtgtcccc aagcggcatg cgcctgctga acggccagcc
     3721 cgtggatgcc aaggagatgc aggcccgctg cgcctacgtg cagcaggacg atctgttcat
     3781 cggcagcctg acagcccgcg agcatctgat ctttcaggcc atggtccgca tgccccgcca
     3841 cctgacatac cgtcagcgcg tggcccgcgt ggaccaggtc atccaggagc tgagcctgag
     3901 caagtgccag cacaccatta ttggcgtgcc aggccgcgtg aagggcctga gtggcggaga
     3961 gcgcaagcgc ctggcctttg ccagcgaggc cctgaccgat ccccccctgc tgatttgcga
     4021 cgagccgacc agcggcctgg atagcttcac cgcccactcc gtggtgcagg tgctgaagaa
     4081 gctgagccag aagggaaaga cggtcattct gacgatccac cagcccagct cggagctgtt
     4141 cgagctgttt gataagattc tgctgatggc cgagggacgc gtggccttcc tgggaacccc
     4201 aagcgaggcc gtggatttct tcagctatgt gggcgcccag tgccccacga attacaaccc
     4261 cgccgacttc tatgtgcaag tgctggccgt ggtgccagga cgcgagattg agagccgcga
     4321 tcgcattgcc aagatctgcg ataacttcgc catctccaag gtggcccgcg acatggagca
     4381 gctgctggcc acgaagaacc tggagaagcc cctggagcag cccgagaacg gctatacgta
     4441 taaggctacc tggtttatgc agtttcgcgc cgtgctgtgg cgcagctggc tgagtgtgct
     4501 gaaggagccc ctgctggtca aggtccgcct gatccagacc acgatggtgg ccattctgat
     4561 cggcctgatc ttcctgggac agcagctgac gcaggtcgga gtgatgaaca tcaatggagc
     4621 catttttctg tttctgacga acatgacgtt ccagaacgtg ttcgccacca tcaacgtgtt
     4681 caccagcgag ctgcccgtgt tcatgcgcga ggcccgcagc cgcctgtacc gctgcgatac
     4741 ctacttcctg ggaaagacca ttgccgagct gccgctgttc ctgaccgtgc ccctggtgtt
     4801 taccgccatt gcctacccca tgattggcct gcgtgccgga gtgctgcatt tctttaattg
     4861 cctggccctg gtgaccctgg tggccaacgt gtcgaccagc ttcggctacc tgatcagctg
     4921 cgcctccagc agcaccagca tggccctgag cgtgggaccc cccgtgatta tccccttcct
     4981 gctgttcggc ggattctttc tgaactccgg cagcgtgccc gtgtacctga agtggctgag
     5041 ctacctgagc tggtttcgct acgccaatga gggcctgctg atcaatcagt gggccgatgt
     5101 ggagcccggc gagatctcct gcaccagcag caacacgacc tgccccagca gcggaaaggt
     5161 catcctggag acactgaatt tcagtgccgc cgacctgcca ctggattatg tgggcctggc
     5221 catcctgatt gtgtccttcc gcgtgctggc ctatctggct ctgcgcctgc gcgctcgtcg
     5281 caaggagtga gcttgatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     5341 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     5401 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     5461 aggttcaggg ggaggtgtgg gaggtttttt cagtatggca agcgctcgac gaattaacaa
     5521 gcttcgctct atctcgctct ctatctctct cgatcgctcg tgctaagctt aagaccgtgg
     5581 agaacaagga gaaccgcttg acacggttat ccacagaatc aggggataac gcaggaaaga
     5641 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5701 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5761 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     5821 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     5881 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     5941 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6001 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6061 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6121 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     6181 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     6241 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6301 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6361 tcatgagatt atcaaaaagg atcttcacct agatcctttt gtgaccagcc aggacagaaa
     6421 tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     6481 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     6541 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     6601 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     6661 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     6721 gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga
     6781 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     6841 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     6901 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     6961 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     7021 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     7081 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     7141 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     7201 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     7261 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     7321 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtagc
     7381 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     7441 acaggcttat cttggacaag aagaagatcg tttggcctcg cgcgcagatc agttggaaga
     7501 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat cactagacca
     7561 atgttacaca tatatacttt agattgattt aaaacttcat gttacgcgcc ctgtagcggc
     7621 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7681 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7741 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     7801 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccagcgcc ctgatagacg
     7861 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7921 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     7981 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     8041 atattaacgc ttacaattt
//
