LOCUS       sequence-331714-        8062 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Blasticidin resistant 5xUAS mCherry GMR-White CDS response vector. 
            Contains an Alpha level GB2.0 entry point for addition of custom 
            assemblies utilizing blue-white bacterial colony screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 8062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pBlastR-5xUAS-mCherry-GMRWhite
FEATURES             Location/Qualifiers
     source          1..8062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1011..1145
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1314..1902)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1394..1413)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             2370..3161
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3216..3671
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3303..3322)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3513..3534
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3695..3742
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        3796..3814
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3796..3813
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    3821..3877
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(3879..3898)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3880..3898)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3905..3922)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3905..3921)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3914..3936)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4130..4149)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    4206..4275
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     4702..4723
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     polyA_signal    7791..7925
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gcttaagacc gtggagaaca aggagaaccg ctgtcaggag cggagtactg tcctccgagc
       61 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
      121 gtactgtcct ccgtcccgag ctcgcccggg gatcgagcgc agcggtataa aagggcgcgg
      181 ggtggctgag agcatcagtt gtgaatgaat gttcgagccg agcagacgtg ccgctgcctt
      241 cgttaatatc ctttgaataa gccaactttg aatcacaaga cgcataccaa accaaatggt
      301 gtccaaggga gaagaggata acatggccat catcaaggag ttcatgcgct tcaaggtcca
      361 catggagggc agcgtgaatg gccacgagtt cgagatcgag ggcgaaggcg agggacgtcc
      421 ctacgaaggc acccagaccg ccaaactgaa ggtgaccaag ggaggcccgt tgccctttgc
      481 ctgggacatc ttgagccccc agttcatgta cggctccaag gcctacgtga agcacccggc
      541 cgacattccc gactacctga agctgagctt ccccgaaggc ttcaagtggg agcgcgtgat
      601 gaacttcgag gacggcggag tggtgaccgt gacccaggac tccagcttgc aggacggcga
      661 gttcatctac aaggtcaagc tgcgcggcac gaactttccc tccgatggcc cggtgatgca
      721 gaagaagacc atgggctggg aggcttccag cgagcgcatg tatcccgagg acggcgctct
      781 gaagggcgag atcaagcagc gcctgaagct gaaggatgga ggccactacg acgccgaggt
      841 gaagaccacc tacaaggcca agaagcccgt gcaattgccc ggcgcctaca acgtgaacat
      901 caagctggac atcaccagcc acaacgagga ctacaccatc gtggagcagt acgagcgtgc
      961 cgaaggacgt catagcacgg gcggcatgga tgagctgtac aagtaagctt gatccagaca
     1021 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     1081 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     1141 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     1201 ttttttcgct tgacacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     1261 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     1321 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1381 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1441 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1501 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1561 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1621 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1681 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1741 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1801 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1861 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1921 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1981 ttatcaaaaa ggatcttcac ctagatcctt ttgtgaccag ccaggacaga aatgcctcga
     2041 cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
     2101 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     2161 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     2221 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     2281 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     2341 gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga
     2401 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     2461 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     2521 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     2581 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     2641 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     2701 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     2761 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     2821 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     2881 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     2941 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgta gctgccgact
     3001 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     3061 atcttggaca agaagaagat cgtttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     3121 actacgtgaa aggcgagatc accaaggtag tcggcaaata atcactagac caatgttaca
     3181 catatatact ttagattgat ttaaaacttc atgttacgcg ccctgtagcg gcgcattaag
     3241 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     3301 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     3361 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     3421 aaaacttgat tagggtgatg gttcacgtag tgggccagcg ccctgataga cggtttttcg
     3481 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     3541 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     3601 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     3661 gcttacaatt ttaacggatc agcgagagac ccctgttgac aattaatcat cggcatagta
     3721 tatcggcata gtataatacg acaaggtgag gaactaaacc caggaggcag atcatgacca
     3781 tgattacgcc aagctattta ggtgacacta tagaatactc aagcttgcat gcctgcaggt
     3841 cgactctaga ggatccccgg gtaccgagct cgaattcgcc ctatagtgag tcgtattaca
     3901 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     3961 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     4021 atcgcccttc ccaacagttg cgcagcctga atggcgaatg acgcctgatg cggtattttc
     4081 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     4141 ctgatgccgc atagggtctc tctccggatc ccagggagga attggtcgac gatgtaggtc
     4201 actgtctcga agccgcggtg cgggtgccag ggcgtgccct tgggctcccc gggcgcgtac
     4261 tccacctcac ccatctggtc catcatgatg cgctgtcagg agctagaatc ccaaaacaaa
     4321 ctggttattg tggtaggtca tttgtttggc agaaagaaaa ctcgagaaat ttctctggcc
     4381 gttattcgtt attctctctt ttctttttgg gtcgctccct ctctgcacta atgctctctc
     4441 actctgtcac acagtaaacg gcatactgct ctcgttggtt cgagagagcg cgcctcgaat
     4501 gttcgcgaaa agagcgccgg agtataaata gaggcgcttc gtctacggag cgacaattca
     4561 attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa acaagcgcag
     4621 ctgaacaagc taaacaatct gcagtaaagt gcaagttaaa gtgaatcaat taaaagtaac
     4681 cagcaaccaa gtaaatcaac tgcaactact gaaatctgcc aagaagtaat tattgaatac
     4741 aagaagagaa cactcaatac tcgaatccat gtgggagttt attcttgaca cagatattta
     4801 tgatataata actgagtaag cttaacataa ggaggaaaac aaaatgaaaa ccttcaacat
     4861 cagccagcag gacctggaac tggtggaggt ggctaccgag aagattacga tgctctacga
     4921 ggataacaag caccacgtgg gagctgccat tcgcaccaag acgggcgaga ttatcagtgc
     4981 cgtgcacatt gaggcctaca tcggacgcgt gaccgtgtgt gccgaggcca ttgccattgg
     5041 cagtgccgtg agcaacggcc agaaggattt tgataccatt gtggccgtgc gtcacccata
     5101 ctccgatgag gtggaccgta gcatccgtgt cgtgtccccg tgtggaatgt gccgtgagct
     5161 gattagcgat tacgcccccg attgcttcgt gctgattgag atgaacggca agctggtcaa
     5221 gaccaccatt gaggaactga ttcccctgaa gtatacccgt aactaagctt ggcaataaaa
     5281 agacagaata aaacgcacgg gtgttgggtc gtttgttccg ctgtcaggag cgtacgacta
     5341 gcacctgact gtcgagccca gtggaaaccc ttgaaatgcc tttaagtcga gcccagtgga
     5401 aacccttgaa atgcctttaa gtcgagccca gtggaaaccc ttgaaatgcc tttaagtcga
     5461 gcccagtgga aacccttgaa atgcctttaa gtcgagccca gtggaaaccc ttgaaatgcc
     5521 tttaagtcga gtagcagtcg aggtaggtac gtcatgcccc tcccgagctc gcccggggat
     5581 cgagcgcagc ggtataaaag ggcgcggggt ggctgagagc atcagttgtg aatgaatgtt
     5641 cgagccgagc agacgtgccg ctgccttcgt taatatcctt tgaataagcc aactttgaat
     5701 cacaagacgc ataccaaacc aaatgggaca ggaggaccag gagctgctga tccgcggagg
     5761 ctccaagcac ccaagcgccg agcacctgaa caacggcgat agcggagccg ccagccagtc
     5821 gtgcatcaat cagggatttg gccaggccaa gaattacgga accctgctgc cccccagccc
     5881 cccagaggat agcggaagcg gatccggaca gctggccgag aatctgacgt acgcctggca
     5941 taacatggat atcttcggcg ccgtgaacca gccaggcagt ggctggcgtc agctggtgaa
     6001 ccgcacccgc ggactgttct gcaatgagcg ccatatccca gccccacgca agcacctgct
     6061 gaagaatgtg tgcggagtgg cctaccccgg cgagctgctg gccgtgatgg gaagtagtgg
     6121 cgccggaaag accacgctgc tgaacgccct ggccttccgc agcccccagg gcattcaggt
     6181 gtccccaagc ggcatgcgcc tgctgaacgg ccagcccgtg gatgccaagg agatgcaggc
     6241 ccgctgcgcc tacgtgcagc aggacgatct gttcatcggc agcctgacag cccgcgagca
     6301 tctgatcttt caggccatgg tccgcatgcc ccgccacctg acataccgtc agcgcgtggc
     6361 ccgcgtggac caggtcatcc aggagctgag cctgagcaag tgccagcaca ccattattgg
     6421 cgtgccaggc cgcgtgaagg gcctgagtgg cggagagcgc aagcgcctgg cctttgccag
     6481 cgaggccctg accgatcccc ccctgctgat ttgcgacgag ccgaccagcg gcctggatag
     6541 cttcaccgcc cactccgtgg tgcaggtgct gaagaagctg agccagaagg gaaagacggt
     6601 cattctgacg atccaccagc ccagctcgga gctgttcgag ctgtttgata agattctgct
     6661 gatggccgag ggacgcgtgg ccttcctggg aaccccaagc gaggccgtgg atttcttcag
     6721 ctatgtgggc gcccagtgcc ccacgaatta caaccccgcc gacttctatg tgcaagtgct
     6781 ggccgtggtg ccaggacgcg agattgagag ccgcgatcgc attgccaaga tctgcgataa
     6841 cttcgccatc tccaaggtgg cccgcgacat ggagcagctg ctggccacga agaacctgga
     6901 gaagcccctg gagcagcccg agaacggcta tacgtataag gctacctggt ttatgcagtt
     6961 tcgcgccgtg ctgtggcgca gctggctgag tgtgctgaag gagcccctgc tggtcaaggt
     7021 ccgcctgatc cagaccacga tggtggccat tctgatcggc ctgatcttcc tgggacagca
     7081 gctgacgcag gtcggagtga tgaacatcaa tggagccatt tttctgtttc tgacgaacat
     7141 gacgttccag aacgtgttcg ccaccatcaa cgtgttcacc agcgagctgc ccgtgttcat
     7201 gcgcgaggcc cgcagccgcc tgtaccgctg cgatacctac ttcctgggaa agaccattgc
     7261 cgagctgccg ctgttcctga ccgtgcccct ggtgtttacc gccattgcct accccatgat
     7321 tggcctgcgt gccggagtgc tgcatttctt taattgcctg gccctggtga ccctggtggc
     7381 caacgtgtcg accagcttcg gctacctgat cagctgcgcc tccagcagca ccagcatggc
     7441 cctgagcgtg ggaccccccg tgattatccc cttcctgctg ttcggcggat tctttctgaa
     7501 ctccggcagc gtgcccgtgt acctgaagtg gctgagctac ctgagctggt ttcgctacgc
     7561 caatgagggc ctgctgatca atcagtgggc cgatgtggag cccggcgaga tctcctgcac
     7621 cagcagcaac acgacctgcc ccagcagcgg aaaggtcatc ctggagacac tgaatttcag
     7681 tgccgccgac ctgccactgg attatgtggg cctggccatc ctgattgtgt ccttccgcgt
     7741 gctggcctat ctggctctgc gcctgcgcgc tcgtcgcaag gagtgagctt gatccagaca
     7801 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     7861 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     7921 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     7981 ttttttcagt atggcaagcg ctcgacgaat taacaagctt cgctctatct cgctctctat
     8041 ctctctcgat cgctcgtgct aa
//
