LOCUS       sequence-331715-        9327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Blasticidin resistant 5xUAS EBFP2 Mini-White CDS response vector. 
            Contains an Alpha level GB2.0 entry point for addition of custom 
            assemblies utilizing blue-white bacterial colony screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9327)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 9327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pBlastR-5xUAS-EBFP2-MiniWhite
FEATURES             Location/Qualifiers
     source          1..9327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(511..1302)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1770..2358
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2259..2278
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3125..3739,3810..3941,4145..4460,4522..5176,
                     5251..5524,5926..5997))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     6449..6467
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     polyA_signal    6555..6689
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6592..6611)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6646..6665
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(8245..8696)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(8276..8297)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    8724..8793
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     8850..8869
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9063..9085
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9077..9094
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9078..9094
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9101..9120
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9101..9119
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    9122..9178
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(9185..9203)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9186..9203)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(9257..9304)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgctggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtaaca tgaagtttta aatcaatcta
      481 aagtatatat gtgtaacatt ggtctagtga ttatttgccg actaccttgg tgatctcgcc
      541 tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaaac gatcttcttc
      601 ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg ccggcaggcg
      661 ctccattgcc cagtcggcag ctacatcctt cggcgcgatt ttgccggtta ctgcgctgta
      721 ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga
      781 gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc
      841 aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag
      901 caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt
      961 gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc
     1021 gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc
     1081 cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt
     1141 caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc
     1201 gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc
     1261 tgatagttga gtcgatactt cggcgatcac cgcttccctc ataatgttta actttgtttt
     1321 agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac
     1381 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtaccccaa aaaaacagtc
     1441 ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct
     1501 ggaccagttg cgtgagcgca tacgctactt gcattacagc ttacgaaccg aacaggctta
     1561 tgtccactgg gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg caaccttggg
     1621 tagcagcgaa gtcgaggcat ttctgtcctg gctggtcaca aaaggatcta ggtgaagatc
     1681 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1741 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1801 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1861 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     1921 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1981 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2041 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2101 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2161 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2221 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2281 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2341 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2401 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtgtc
     2461 aagcgatgca tctacacaag gaacaaacac tggatgtcac tttcagttca aattgtaacg
     2521 ctaatcactc cgaacaggtc acaaaaaatt accttaaaaa gtcataatat taaattagaa
     2581 taaatatagc tgtgagggaa atatatacaa atatattgga gcaaataaat tgtacataca
     2641 aatatttatt actaatttct attgagccga aatgaaccac tcggaaccat ttgagcgaac
     2701 cgaatcgcgc ggaactaacg acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt
     2761 gcggagagcg ggtgggagac agcgaaagag caactacgaa acgtggtgtg gtggaggtga
     2821 attatgaaga gggcgcgcga tttgaaaagt atgtatataa aaaatatatc ccggtgtttt
     2881 atgtagcgat aaacgagttt ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga
     2941 agacaaatcc aaagtctact tgtggggatg ttcgaagggg aaatacttgt attctatagg
     3001 tcatatcttg tttttattgg cacaaatata attacattag ctttttgagg gggcaataaa
     3061 cagtaaacac gatggtaata atggtaaaaa aaaaaaacaa gcagttattt cggatatatg
     3121 tcggctactc cttgcgtcgg gcccgaagtc ttagagccag atatgcgagc acccggaagc
     3181 tcacgatgag aatggccaga cccacgtagt ccagcggcag atcggcggcg gagaagttaa
     3241 gtgtctccag gatgaccttg cccgaactgg ggcacgtggt gttcgacgat gtgcagctaa
     3301 tttcgcccgg ctccacgtcc gcccattggt taatcagcag accctcgttg gcgtaacgga
     3361 accatgagag gtacgacaac catttgaggt atactggcac cgagcccgag ttcaagaaga
     3421 agccgccaaa gagcaggaat ggtatgataa ccggcggacc cacagacagc gccatcgagg
     3481 tcgaggagct ggcgcaggat attagatatc cgaaggacgt tgacacattg gccaccagag
     3541 tgaccagcgc caggcagttg aagaagtgca gcactccggc ccgcagtccg atcatcggat
     3601 aggcaatcgc cgtgaagacc agtggcactg tgagaaaaag cggcaattcg gcaatcgttt
     3661 tgcccagaaa gtatgtgtca cagcgataaa gtcgacttcg ggcctccctc ataaaaactg
     3721 gcagctctga ggtgaacacc taaatcgaat cgattcatta gaaagttagt aaattattga
     3781 aatgcaaatg tattctaaac atgacttaca tttatcgtgg caaagacgtt ttgaaaggtc
     3841 atgttggtca ggaagaggaa gatggctccg ttgatattca tcacacccac ttgcgtgagt
     3901 tgttggccca aaaagatgag gccaatcaag atggcaacca tctgcaaatt aaaatgttac
     3961 tcgcatctca ttaatattcg cgagttaaat gaaatttatt tatcttctgc aaaactataa
     4021 actatacatc tcattgaaaa aaactaagaa gggtgtggaa tcaggcaatt ctatctaaaa
     4081 tctagcgaat ttgtttccaa gaattgtaag cgttatatca tttgtttcca ctggaaccac
     4141 tcaccgttgt ctgaataagt cgcactttta cgaggagtgg ttccttgagc accgacagcc
     4201 aggatcgcca caggaccgcc cggaactgca tgaaccaggt ggccttgtag gtgtacccat
     4261 tctccggctg ctccagtggc ttctccagat ttttggtggc caacaactgc tccatatccc
     4321 gggctacttt gctaatggca aaattgtcgc atatcttggc gatccgatca cgggactcga
     4381 tctcccgtcc gggcacaacg gccaacacct gtacgtaaaa gtccgccgga ttgtagttgg
     4441 taggacactg ggcacccacg ctggatagga gttgagatgt tatgtaatac tagataccct
     4501 taataaacac atcgaactca ctaggaaaag aagtcgacgg cttcgctggg agtgcccaag
     4561 aaagctaccc tgccctcggc catcagaagg atcttgtcaa agagctcaaa cagctcggaa
     4621 gacggctgat gaatggtcag gatgacggtc ttgcccttct gcgacagctt cttcagcacc
     4681 tggacgacgc tgtgggcggt aaaggagtcc agtccggagg tgggctcatc gcagatcaga
     4741 agcggcggat cggttagagc ctcggaggcg aatgccagac gcttcctttc tccgccggac
     4801 agacctttca ccctgccggg cacaccgatg atcgtgtgct gacatttgct gagcgaaagc
     4861 tcctggatca cctgatccac gcgggccact cgctgccgat aggtcagatg tcgtggcatc
     4921 cgcaccatgg cttggaaaat caggtgttcc ctggccgtta gggagccgat aaagaggtca
     4981 tcctgctgga cataggcgca cctggcctgc atctccttgg cgtccacagg ttggccattg
     5041 agcagtcgca tcccggatgg cgatacttgg atgccctgcg gcgatcgaaa ggcaagggca
     5101 ttcagcaggg tcgtctttcc ggcaccggaa ctgcccatca cggccaaaag ttcgcccgga
     5161 taggccacgc cgcaaactga gtttcaaatt ggtaattgga ccctttatta agatttcaca
     5221 cagatcagcc gactgcgaat agaaactcac cgttcttgag caaatgtttc ctgggcgccg
     5281 gtatgtgtcg ctcgttgcag aatagtccgc gtgtccggtt gaccagctgc cgccatccgg
     5341 agcccggctg attgaccgcc ccaaagatgt ccatattgtg ccaggcatag gtgaggttct
     5401 cggctagttg gccgctccct gaaccggagt cctccggcgg actgggtggc cggagcgtgc
     5461 cgtagttttt ggcctgcccg aagccctggt taatgcagct ctgcgaagcc gctccgctgt
     5521 caccctgcaa tgatagggga tctcaaatat caactacaag cgttatgctc atctaacccc
     5581 gaacaaaaag taccccgaag tatcctacga agtaggttta tacttttatt tattttttgt
     5641 gcatctagga tcagcttaaa atatctggtt gttatatttt ttgtaaaaaa gaatatagtc
     5701 gaaaatgaat gcctttagat gtcttgatca tgatatgatc tcaaaaattg tcttatatag
     5761 cgagaacagc taccagaata atctgtttcg tgtcactatt tgtttgtgca attgcggttt
     5821 gggatttttg tgggtcgcag ttctcacgcc gcagacaatt tgatgttgca atcgcagttc
     5881 ctatagatca agtgaactta agatgtatgc acatgtacta ctcacattgt tcagatgctc
     5941 ggcagatggg tgtttgctgc ctccgcgaat taatagctcc tgatcctctt ggcccattgc
     6001 cgggattttt cacactttcc cctgcttacc cacccaaaac caatcaccac cccaatcact
     6061 caaaaaacaa acaaaaataa gaagcgagag gagttttggc acagcacttt gtgtttaatt
     6121 gatggcgtaa accgcttgga gcttcgtcac gaaaccgctg acaaaatgca actgaaggcg
     6181 gacattgacg ctacgtaacg ctacaaacgg tggcgaaaga gatagcggac gcagcggcga
     6241 aagtgacggc gatatttctg tggacagaga aggaggcaaa cagcgctgac tttgagtgga
     6301 atgtcatttt gagtgagagg taatcgaaag aacctggtac atcaaatacc cttggatcga
     6361 agtaaattta aaactgatca gataagttca atgatatcca gtgcagtaaa aaaaaaaaat
     6421 gtttttttta tctactttcc gcaaaaatgg gttttattaa cttacataca tactagaatt
     6481 cctcctgaca gcgaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     6541 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6601 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6661 tcatcaatgt atcttatcat gtctggatca agcttacttg tacagctcat ccatgcccag
     6721 ggtgatgccg gcggcggtgc ggaactccag cagcaccatg tgatcgcgct tctcgttggg
     6781 atccttggac agcacgctct gggtgctcag gtagtggcta tcgggcagca gcacggggcc
     6841 gtcgccgatg ggggtgttct gctggtagtg atcggccagc tgcactgagc catcctccac
     6901 gttgtggcgg atcttgaagt tgaccttgat gccgttcttc tgcttcacgg ccatgatgta
     6961 gatgttgtgg ctgttgaagt tgtactccag cttgtggccc aggatgttgc catcctcctt
     7021 gaaatccacg cccttcagct cgatgcggtt caccagggta tcgccctcga acttcacctc
     7081 ggcgcgggtc ttgtaggtgc cgtcgtcatt gaagaaaatg gtgcgctcct gcacgtagcc
     7141 ctcgggcatg gcgctcttga agaaatcgtg ctgcttcatg tgatcggggt agcgggcgaa
     7201 gcactgcacg ccgtggctca gggtggtcac cagggtgggc cagggcacgg gcagcttgcc
     7261 ggtggtgcag atgaacttca gggtcagctt gccgttggtg gcgtcgccct cgccctcgcc
     7321 gcgcacgctg aacttgtggc cgttcacgtc gccatccagc tccaccagga tgggcaccac
     7381 gccggtgaac agctcctcgc ccttggacac catttggttt ggtatgcgtc ttgtgattca
     7441 aagttggctt attcaaagga tattaacgaa ggcagcggca cgtctgctcg gctcgaacat
     7501 tcattcacaa ctgatgctct cagccacccc gcgccctttt ataccgctgc gctcgatccc
     7561 cgggcgagct cgggacggag gacagtactc cgctcggagg acagtactcc gctcggagga
     7621 cagtactccg ctcggaggac agtactccgc tcggaggaca gtactccgct cctgacagcg
     7681 gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt ttattgccaa gcttagttac
     7741 gggtatactt caggggaatc agttcctcaa tggtggtctt gaccagcttg ccgttcatct
     7801 caatcagcac gaagcaatcg ggggcgtaat cgctaatcag ctcacggcac attccacacg
     7861 gggacacgac acggatgcta cggtccacct catcggagta tgggtgacgc acggccacaa
     7921 tggtatcaaa atccttctgg ccgttgctca cggcactgcc aatggcaatg gcctcggcac
     7981 acacggtcac gcgtccgatg taggcctcaa tgtgcacggc actgataatc tcgcccgtct
     8041 tggtgcgaat ggcagctccc acgtggtgct tgttatcctc gtagagcatc gtaatcttct
     8101 cggtagccac ctccaccagt tccaggtcct gctggctgat gttgaaggtt ttcattttgt
     8161 tttcctcctt atgttaagct tactcagtta ttatatcata aatatctgtg tcaagaataa
     8221 actcccacat ggattcgagt attgagagtt ctcttcttgt attcaataat tacttcttgg
     8281 cagatttcag tagttgcagt tgatttactt ggttgctggt tacttttaat tgattcactt
     8341 taacttgcac tttactgcag attgtttagc ttgttcagct gcgcttgttt atttgcttag
     8401 ctttcgctta gcgacgtgtt cactttgctt gtttgaattg aattgtcgct ccgtagacga
     8461 agcgcctcta tttatactcc ggcgctcttt tcgcgaacat tcgaggcgcg ctctctcgaa
     8521 ccaacgagag cagtatgccg tttactgtgt gacagagtga gagagcatta gtgcagagag
     8581 ggagcgaccc aaaaagaaaa gagagaataa cgaataacgg ccagagaaat ttctcgagtt
     8641 ttctttctgc caaacaaatg acctaccaca ataaccagtt tgttttggga ttctagctcc
     8701 tgacagcgca tcatgatgga ccagatgggt gaggtggagt acgcgcccgg ggagcccaag
     8761 ggcacgccct ggcacccgca ccgcggcttc gagacagtga cctacatcgt cgaccaattc
     8821 ctccctggga tccggagaga gaccctatgc ggcatcagag cagattgtac tgagagtgca
     8881 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgtca
     8941 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     9001 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     9061 ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg
     9121 cgaattcgag ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagcttga
     9181 gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatgatct gcctcctggg
     9241 tttagttcct caccttgtcg tattatacta tgccgatata ctatgccgat gattaattgt
     9301 caacaggggt ctctcgctga tccgtta
//
