LOCUS       sequence-331718-        1223 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector for CMV promoter driven expression of dominant 
            negative (DN) MAML1 (Notch pathway) fused to mVenus fluorescent 
            protein (to be used in conjunction with Phoenix packaging cells)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Hoare M, Ito Y, Kang TW, Weekes MP, Matheson NJ, Patten DA, Shetty 
            S, Parry AJ, Menon S, Salama R, Antrobus R, Tomimatsu K, Howat W, 
            Lehner PJ, Zender L, Narita M
  TITLE     NOTCH1 mediates a switch between two distinct secretomes during 
            senescence.
  JOURNAL   Nat Cell Biol. 2016 Sep;18(9):979-92. doi: 10.1038/ncb3397. Epub 
            2016 Aug 15.
  PUBMED    27525720
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3397"; 
            journalName: "Nat Cell Biol"; date: "2016-09"; volume: "18"; issue: 
            "9"; pages: "979-92"
COMMENT     Sequence Label: pLPC-dnMAML1-mVenus
FEATURES             Location/Qualifiers
     source          1..1223
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      74..83
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(293..295,296..298,299..811)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(338..359)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(599..618)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     945..966
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     gap             1142..1157
                     /estimated_length=16
ORIGIN
        1 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agcttggtac
       61 cgagctcgga tccgccacca tggcgctgcc gcggcacagc gcggtcatgg agcgccttcg
      121 ccggcgcatc gagctgtgcc ggcgccacca cagcacctgc gaggcccgct acgaggccgt
      181 gtcgcccgag cgcctggagc tggagcgcca acacaccttc gccctgcacc agcgctgcat
      241 ccaggccaag gccaagcgcg ccgggaagca cgggaattct ggcagatcta tcatggtgag
      301 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      361 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      421 gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      481 caccctgggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
      541 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      601 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      661 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
      721 gtacaactac aacagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa
      781 ggccaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta
      841 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      901 ctaccagtcc aagctgagca agaccccaac gagaagcgcg atcacatggt cctgctggag
      961 ttcgtgaccg ccgccgggat cactctcggc atggncgagc tgnacaagta agtcnagcat
     1021 gcatctagag ggccganaaa nnaannattt tatttagtcn ncngaaanng ggggangnaa
     1081 gaccccaccn nnnggnttnn nngctngcta ntacgccatt ttgcnnnnnn naaannnnnn
     1141 tnnnnnnnnn nnnnnnnann annnnggann antnnnnnct nannnnnnng nnncnnnnnn
     1201 ancannnnnn nncnngnnnn ngg
//
