LOCUS       sequence-331721-        9514 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9514)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1032
FEATURES             Location/Qualifiers
     source          1..9514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2452..3040
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2505..2525)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2923..2934)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3112..3345
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3423..3544
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3460..3479)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3514..3533
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3584..3719
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3739..3758)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3740..3758)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3768..3785)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3768..3784)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3777..3799)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3926..4381
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4013..4032)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4223..4244
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4407..4511
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4512..4580,4581..5372)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4730..4749)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5543..6131
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6032..6051
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6285..6302
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6419..6440
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6455..6460,6461..6478,6479..6485)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6493..6509
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6498..6520
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6517..6533
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6517..6533
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6552..6572
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6554..6572
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6600..6826
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6827..7007
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7054..7179
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7672..7905
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8090..8134
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8365..8575
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8387..8583
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8434..8569
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8615..9217
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8615..8634)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9111..9131
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9260..9377
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggt
     1201 gcccagctcg ccctcgcccc ctccgcctcc tcgggtctac aagccatgct tcgtgtgcaa
     1261 tgacaagtcc tctggctacc actatggggt cagctcttgt gaaggctgca agggcttctt
     1321 tcgccgaagc atccagaaga acatggtgta cacgtgtcac cgcgacaaaa actgtatcat
     1381 caacaaggtg accaggaatc gctgccagta ctgccggcta cagaagtgct tcgaagtggg
     1441 catgtccaag gaagctgtgc gaaatgaccg gaacaagaag aagaaagagg tgaaggaaga
     1501 agggtcacct gacagctatg agctgagccc tcagttagaa gagctcatca ccaaggtcag
     1561 caaagcccat caggagactt tcccctcgct ctgccagctg ggcaagtata ccacgaactc
     1621 cagtgcagac caccgcgtgc agctggatct ggggctgtgg gacaagttca gtgagctggc
     1681 taccaagtgc atcatcaaga tcgtggagtt tgccaagcgg ttgcctggct ttacagggct
     1741 cagcattgct gaccagatca ctctgctcaa agctgcctgc ctagatatcc tgatgctgcg
     1801 tatctgcaca aggtacaccc cagagcagga caccatgacc ttctccgacg ggctgaccct
     1861 gaaccggacc cagatgcaca atgccggctt cgggcccctc acagaccttg tctttgcctt
     1921 tgctgggcag ctcctgcccc tggagatgga tgacaccgag acagggctgc tcagcgccat
     1981 ctgcctcatc tgcggagacc gcatggacct ggaggagccc gaaaaagtgg acaagctgca
     2041 ggagccactg ctggaagccc tgaggctgta cgcccggcgc cggcggccca gccagcccta
     2101 catgttccca aggatgctaa tgaaaatcac cgacctccgg ggcatcagca ctaagggagc
     2161 tgaaagggcc attactctga agatggagat tccaggcccg atgcctccct taatccgaga
     2221 gatgctggag aaccctgaaa tgtttgagga tgactcctcg cagcctggtc cccaccccaa
     2281 tgcctctagc gaggatgagg ttcctggggg ccagggcaaa gggggcctga agtccccagc
     2341 ctagtaatga actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     2401 tggaaaggac gactgcggcc ataatgtttc ctatggacgc gttaagtcga caatcaacct
     2461 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     2521 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     2581 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     2641 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     2701 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     2761 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     2821 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     2881 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     2941 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     3001 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     3061 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     3121 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     3181 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     3241 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     3301 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     3361 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     3421 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     3481 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     3541 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     3601 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     3661 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     3721 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     3781 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     3841 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     3901 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3961 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4021 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4081 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4141 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4201 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4261 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4321 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     4381 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4441 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4501 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4561 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4621 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4681 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4741 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4801 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     4861 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     4921 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     4981 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5041 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5101 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5161 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5221 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5281 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5341 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5401 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5461 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5521 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5581 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5641 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     5701 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5761 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5821 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5881 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5941 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6001 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6061 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6121 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6181 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     6241 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6301 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6361 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     6421 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     6481 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     6541 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     6601 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     6661 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     6721 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     6781 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     6841 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     6901 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6961 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     7021 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     7081 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     7141 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     7201 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     7261 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     7321 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     7381 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     7441 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     7501 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     7561 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     7621 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     7681 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     7741 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     7801 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     7861 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     7921 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     7981 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     8041 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     8101 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     8161 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     8221 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     8281 gacccacctc ccaaccccga ggggacccat gcattgcatc tcaattagtc agcaaccagg
     8341 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgcg tctcaattag
     8401 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8461 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8521 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     8581 aaaaagcttt ctagaggtac caccggagct taccatgacc gagtacaagc ccacggtgcg
     8641 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     8701 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     8761 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     8821 cggcgccgcc gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     8881 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     8941 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     9001 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     9061 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     9121 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     9181 cacctggtgc atgacccgca agcccggtgc cagttaacga attctagatc ttgagacaaa
     9241 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     9301 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     9361 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc
     9421 tcgaggcctg caggtgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta
     9481 atggaccttc taggtcttga aaggagtggg aatt
//
