LOCUS       sequence-331732-        8875 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses His6- and MBP-tagged BhaB5 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8875)
  AUTHORS   Daniels PN, Lee H, Splain RA, Ting CP, Zhu L, Zhao X, Moore BS, van 
            der Donk WA
  TITLE     A biosynthetic pathway to aromatic amines that uses glycyl-tRNA as 
            nitrogen donor.
  JOURNAL   Nat Chem. 2022 Jan;14(1):71-77. doi: 10.1038/s41557-021-00802-2. 
            Epub 2021 Nov 1.
  PUBMED    34725492
REFERENCE   2  (bases 1 to 8875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41557-021-00802-2"; journalName: "Nat Chem"; date: 
            "2022-01"; volume: "14"; issue: "1"; pages: "71-77"
COMMENT     Sequence Label: pET28b-MBP-bhaB5
FEATURES             Location/Qualifiers
     source          1..8875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             100..117
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             127..144
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             148..1248
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1105..1114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1222..1245
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1261..1278
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1294..1311
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1312..1332
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             2029..2040
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     RBS             3600..3608
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             3661..3690
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="ASMTGGQQMG"
     CDS             3740..3757
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3810..3828)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3824..3871
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      3908..4363
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3995..4014)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4205..4226
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4455..5270)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4455..5270)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5178..5197
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5262..5281
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      5392..5980
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5881..5900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6134..6151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6166..6305
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6231..6250)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6391..6413
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6407..6598)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7407..8489)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8451..8470
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8490..8567)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc
      121 agcggcctgg tgccgcgcgg cagccatatg aaaatcgaag aaggtaaact ggtaatctgg
      181 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat
      241 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt
      301 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac
      361 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat
      421 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt
      481 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa
      541 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac
      601 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag
      661 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg
      721 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac
      781 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg
      841 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc
      901 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt
      961 ccgaacaaag agctggcgaa agagttcctc gaaaactatc tgctgactga tgaaggtctg
     1021 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag
     1081 ttggcgaaag atccacgtat tgccgccacc atggaaaacg cccagaaagg tgaaatcatg
     1141 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc
     1201 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcc
     1261 caccatcacc atcaccacgc gaattcggta ccgctggttc cgcgtggatc tgagaacctg
     1321 tacttccaat ccatgttgtc agtgaaacaa tctagtataa atgaagacca taataactgg
     1381 gatttttttg ggcattttgt tttacggagc acaggcttcc cttatgaatg gatgaaggaa
     1441 cttaaaatgc aacaaacctt tgacttaatc tttcaaaatg cgaaatggga acaggtagag
     1501 tctaagttca atcaagagtt agcaataaaa agagagcagt taaaggctta cttcgattcg
     1561 gaagactttc gtcaagctgt gtttatatct aatcccgata tgtatcaaca tatagaccga
     1621 tatatgaagc attttcaatc tcattcacgc ccatcaaaag tgaaaagaat tgaaaaaaaa
     1681 ttatttacgt accttcagcg cttttgtggc aagaatgaat cggcaagttt tttcggacca
     1741 ttaaattacg gtcaggttga gccaaatatt gacgagtatt gggacggatc gtttattgag
     1801 actaaagatt tacaaaagcg ggaagcattc ttatcctatt gggctgttaa ggcattggca
     1861 aaagcggttg caaaagagaa tgagttagca ccatatgtcc ctttgcaaat cccgtcctgg
     1921 attgtcgtac gcaaggagta tgttgtgttg tcctccggaa aacggattaa tcttccggct
     1981 tggatgatgg aaatcatgca ttatattcaa gaaacttctt cttgtttatg ggaacttcaa
     2041 aatcatttca atcatatcga agcaggacaa ttgaaagcat cgttagagaa attaatttct
     2101 aaaggactca ttcatagaga gtggataatc ccttccaccg ttgtacatcc actacaccgt
     2161 ttgcttgagc agctaaggga gcttccagat agccctgcga agaataaatg gtgtcaggca
     2221 cttgacgaat tagcatctga ggttactaaa ctagctaact taccgatcgt agaaaagcga
     2281 agaagttttg ctcacttaga agaaacgttt accaagctaa caggagaacc tagtcgtaga
     2341 ggaaaagctt ctctttacgc tgatcgcttc atctactatg aagatgcaca agggcatata
     2401 caagaattta gattcggtaa accttttatt gaggatttac aaacgaagct cgctggatct
     2461 ttgaatatga gcgctgctta tggagaggaa atatgggctt actatcaaga attaggaaga
     2521 aacgtgtatg aggacatgca ggtagaggcg cgtaacgatg aaaagagaca actcgccaat
     2581 tctggaatcc ctttttcgtc gttcatcaac aaactgcgcc aaacgtaccc agatgtccct
     2641 caactaccaa aatcatcgtt ttcgaataaa atagaggcga ttattagaga gaaagggact
     2701 gaacagcgtg ttgttaagct tacttctgat caattaaatg ttttcccttc gaatcgttcc
     2761 ttttattctt taccggattt gtttttacaa gcggaaaata tagaagcgtt acgtaacgga
     2821 gacgtgcaaa tcattctcgc aaagctacac caccacttgt taatgcataa ttggatgact
     2881 tatttttatc aagaaaaaga gagacttgaa agggacctgg ttcaactcgt acagaagctt
     2941 gatcatgaag atggaaccgt cctatcgggc cttgaaatca tgagaagaaa caaagcttac
     3001 tatgattatc caacaacagt cattgaatat gcagagaagc cggactctag caaagagtcg
     3061 atcaagctta cggatcttat tgtggttcgc aatgatgacg gacaccttga actacaagaa
     3121 aaaaacactt ccagacctat tgaattatat gttccacttg ctgatcaagt gcactatcta
     3181 ccttttgcga tgttttccaa accgatgctt ctccatgtcc cgatttctag cgggaaacat
     3241 acaccacgaa ttgtaattga tgatgtcgta taccaaagag aacgatggtt tttttataca
     3301 aaacagctgg tagatctgtt tcaccaacta caagggcctt tgctattaaa aaaagttgag
     3361 gaatggagac aggcagaagg aataccagag gtggtttaca tcaagggttc tgatgttcga
     3421 aagccttatt gggtcgattt taaaaactat ttttctcttg agttaatgca gcaaatttta
     3481 ctagaaaata atgagattac aatagaggaa atgctgccag atcctcatca tttgtggcta
     3541 aagtcaagaa aagggagtca ctcgtgcgag ttacggatga gtgtttataa attggggatt
     3601 aaggaggttt ctgaacatgc gtgagcaatt cagggtagcg gtgatgttca ggcagaataa
     3661 gctagcatga ctggtggaca gcaaatgggt cgcggatccg aattcgagct ccgtcgacaa
     3721 gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc
     3781 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     3841 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc
     3901 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     3961 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4021 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4081 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4141 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4201 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     4261 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4321 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     4381 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4441 tcatgaatta attcttagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata
     4501 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca
     4561 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca
     4621 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca
     4681 ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact
     4741 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta
     4801 ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta
     4861 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca
     4921 cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg
     4981 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat
     5041 tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg
     5101 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca
     5161 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg
     5221 gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa tatggctcat aacacccctt
     5281 gtattactgt ttatgtaagc agacagtttt attgttcatg accaaaatcc cttaacgtga
     5341 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5401 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5461 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5521 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5581 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5641 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     5701 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5761 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5821 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5881 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5941 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6001 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     6061 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     6121 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
     6181 tctccttacg catctgtgcg gtatttcaca ccgcaatggt gcactctcag tacaatctgc
     6241 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     6301 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     6361 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     6421 cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg
     6481 attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta
     6541 atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg
     6601 atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga
     6661 ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg
     6721 gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc
     6781 agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc
     6841 agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac
     6901 ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc
     6961 ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc
     7021 ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggggcc gccatgccgg
     7081 cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag
     7141 cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc
     7201 gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca
     7261 tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg
     7321 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga
     7381 gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     7441 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc
     7501 agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg
     7561 ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt
     7621 ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact
     7681 accgagatat ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc
     7741 gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc
     7801 atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga
     7861 atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa
     7921 cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg
     7981 cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag
     8041 acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg
     8101 tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc
     8161 gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc
     8221 agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga
     8281 ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg
     8341 ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa
     8401 acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct
     8461 gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg
     8521 cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg
     8581 ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag
     8641 caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac
     8701 ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg
     8761 atcttcccca tcggtgatgt cggcgatata tgcgccagca accgcacctg tggcgccggt
     8821 gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
//
