LOCUS       sequence-331740-       10218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for fusion of GFP with human PGBD5, 
            NCBI: NM_001258311.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10218)
  AUTHORS   Henssen AG, Koche R, Zhuang J, Jiang E, Reed C, Eisenberg A, Still 
            E, MacArthur IC, Rodriguez-Fos E, Gonzalez S, Puiggros M, Blackford 
            AN, Mason CE, de Stanchina E, Gonen M, Emde AK, Shah M, Arora K, 
            Reeves C, Socci ND, Perlman E, Antonescu CR, Roberts CWM, Steen H, 
            Mullen E, Jackson SP, Torrents D, Weng Z, Armstrong SA, Kentsis A
  TITLE     PGBD5 promotes site-specific oncogenic mutations in human tumors.
  JOURNAL   Nat Genet. 2017 May 15. doi: 10.1038/ng.3866.
  PUBMED    28504702
REFERENCE   2  (bases 1 to 10218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2017 May 15. doi: 10.1038/ng.3866."
COMMENT     Sequence Label: pRECLV103_PGBD5_B
FEATURES             Location/Qualifiers
     source          1..10218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        404..783
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        784..987
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     938..958
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     984..1008
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(1061..1063,1064..1066,1067..1777)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1061..1063,1064..1066,1067..1777)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1106..1127)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1367..1386)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1714..1735
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3381..3405)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3480..3597
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3646..4156
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4075..4093
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4178..4777
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4178..4197)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4674..4694
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4905..5138
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5210..5331
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5247..5266)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5301..5320
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5371..5506
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5526..5545)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5527..5545)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5555..5572)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5555..5571)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5564..5586)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5713..6168
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5800..5819)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6010..6031
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6194..6298
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6299..6367,6368..7159)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6517..6536)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7330..7918
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7819..7838
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8072..8089
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8206..8227
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8242..8247,8248..8265,8266..8272)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8280..8296
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8285..8307
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8304..8320
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8304..8320
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8339..8359
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8341..8359
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8387..8613
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8614..8794
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8841..8966
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9459..9692
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9877..9921
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg gtatctatac attgaatcaa tattggcaat tagccatatt agtcattggt
      301 tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat
      361 atgtacattt atattggctc atgtccaata tgaccgccat gttgacattg attattgact
      421 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      481 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      541 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      601 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      661 agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      721 atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      781 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      841 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      901 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      961 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     1021 catccacgct gttttgacct ccatagaaga ttctagaacc atggtgagca agggcgagga
     1081 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1141 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     1201 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     1261 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     1321 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     1381 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     1441 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     1501 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     1561 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     1621 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     1681 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1741 cgccgggatc actctcggca tggacgagct gtacaaggtt cgaaacacca tggccgaggg
     1801 cggcgggggc gcgcggagga gggcgccggc gctgctcgag gctgcccgcg cgcgctacga
     1861 gagcctgcac atctcggacg acgtgttcgg cgagtccggc ccggacagcg gcgggaaccc
     1921 cttctacagc acctcggccg cctcgcgctc ctcctcggcc gcctcctcgg acgacgagcg
     1981 cgagcccccg ggacccccag gggccgcccc gccaccgccc cgcgccccgg acgcacagga
     2041 gccggaggag gacgaggccg gcgcgggctg gagcgcagcg ctgcgggacc gcccgccccc
     2101 gcgcttcgag gataccggcg gtcccacccg aaagatgccc cccagcgcca gtgccgtgga
     2161 cttcttccag ctctttgtcc cagacaacgt cctcaagaac atggtggtgc agacaaacat
     2221 gtatgccaag aagttccagg agcggtttgg gagcgacgga gcctgggtgg aggtgacgct
     2281 gacggagatg aaggcgttcc tgggctacat gatctccacc agcatctccc actgcgagtc
     2341 cgtcctcagc atctggagcg gaggcttcta cagcaaccgc agcctcgccc tcgtcatgag
     2401 ccaggcccgc ttcgagaaga tcctcaagta cttccacgtc gtggccttcc gctccagcca
     2461 gaccacgcac gggctctaca aggtccagcc cttcctcgac tccctgcaga acagcttcga
     2521 ctctgccttc aggccttccc aaacccaggt gctacatgaa cccctgatcg atgaggatcc
     2581 tgtattcatt gccacgtgca cagagcggga gctgcgaaag aggaaaaagc ggaaattcag
     2641 cctctgggtc agacaatgtt cttccactgg cttcatcatc cagatttatg tccacctgaa
     2701 ggaaggtggg ggcccagatg gcctggatgc gctgaagaat aagccccagc tccacagcat
     2761 ggtggccagg agcctgtgcc ggaacgcggc aggcaagaac tacatcattt tcacggggcc
     2821 cagcatcacc agcctgacgc tgtttgaaga gtttgagaag caagggattt actgctgcgg
     2881 cttgctccgc gcgcggaaga gtgactgcac cggcctccca ctgtccatgc tgaccaaccc
     2941 agccacaccc ccggcccggg gccagtacca aatcaagatg aaggggaaca tgtccttgat
     3001 ctgctggtac aacaaaggac acttccgctt cctgaccaac gcctactccc cggtgcagca
     3061 gggagtcatc atcaaaagga agagtgggga gatcccatgc cccttggccg tggaggcgtt
     3121 tgccgctcac ctgagctaca tctgcagata cgatgacaaa tacagcaagt atttcatttc
     3181 tcataaacca aacaagacct ggcagcaggt gttctggttc gccatcagca tcgccatcaa
     3241 caatgcctac atcctgtaca aaatgtcaga cgcctaccac gtgaagaggt acagccgggc
     3301 gcagtttgga gagagactcg tcagagagct gctgggcttg gaggatgcct ctccgaccca
     3361 ctagctcgag tgcggccgca acccagcttt cttgtacaaa gtggttgatc gcgtgcatgc
     3421 gacgtcatag ctctctccca attctcgacc tcgagacaaa tggcagtatt catccacaat
     3481 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     3541 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     3601 gtttattaca gggacagcag agatccactt tggccgcggc tcgagggggt tggggttgcg
     3661 ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg
     3721 gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac
     3781 ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag
     3841 tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct
     3901 cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg
     3961 ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc
     4021 ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc
     4081 cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac
     4141 cgacctctct ccccaggggg atccaccgga gcttaccatg accgagtaca agcccacggt
     4201 gcgcctcgcc acccgcgacg acgtccccag ggcggtacgc accctcgccg ccgcgttcgc
     4261 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     4321 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     4381 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     4441 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     4501 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     4561 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     4621 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     4681 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     4741 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg
     4801 cccgaccgaa aggagcgcac gaccccatgc atcggtacct ttaagaccaa tgacttacaa
     4861 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     4921 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     4981 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5041 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5101 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     5161 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     5221 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5281 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     5341 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5401 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     5461 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     5521 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     5581 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     5641 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     5701 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     5761 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     5821 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     5881 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     5941 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6001 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     6061 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     6121 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     6181 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     6241 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     6301 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6361 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6421 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6481 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6541 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6601 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6661 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6721 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6781 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6841 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6901 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6961 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     7021 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7081 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     7141 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     7201 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7261 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7321 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7381 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7441 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     7501 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7561 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7621 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7681 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7741 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7801 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7861 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7921 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7981 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8041 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     8101 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     8161 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     8221 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     8281 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     8341 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     8401 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     8461 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     8521 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     8581 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
     8641 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     8701 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8761 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     8821 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     8881 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     8941 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     9001 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     9061 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     9121 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     9181 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     9241 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     9301 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     9361 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     9421 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     9481 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     9541 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     9601 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     9661 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     9721 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     9781 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     9841 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     9901 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     9961 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
    10021 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
    10081 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
    10141 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
    10201 gcggccgccc ccttcacc
//
