LOCUS       sequence-331744-       11975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral transfer vector for expression of roGFP2 and HyPer Red.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11975)
  AUTHORS   Faber SC, McNabb NA, Ariel P, Aungst ER, McCullough SD
  TITLE     Exposure Effects Beyond the Epithelial Barrier: Trans-Epithelial 
            Induction of Oxidative Stress by Diesel Exhaust Particulates in Lung
            Fibroblasts in an Organotypic Human Airway Model.
  JOURNAL   Toxicol Sci. 2020 Jun 11. pii: 5856111. doi: 10.1093/toxsci/kfaa085.
  PUBMED    32525552
REFERENCE   2  (bases 1 to 11975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Toxicol 
            Sci. 2020 Jun 11. pii: 5856111. doi: 10.1093/toxsci/kfaa085."
COMMENT     Sequence Label: pLV-dfROX
FEATURES             Location/Qualifiers
     source          1..11975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    1227..1344
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      1401..1410
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1407..1409,1410..1412,1413..2123)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1452..1473)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1713..1732)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2060..2081
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2133..2186
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2187..2567,2568..2576,2577..3302,3303..3311,
                     3312..3614)
                     /codon_start=1
                     /product="red fluorescent genetically encoded indicator for
                     H2O2 ()"
                     /label=HyPerRed
                     /translation="MEMASQQGETMSGPLHIGLIPTVGPYLLPHIIPMLHQTFPKLEMY
                     LHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPLFDEPMLLAIYEDHPWANRECVP
                     MADLAGEKLLMLEDGHCLRDQAMPRTVSERMYPEDGALKSEIKKGLRLKDGGHYAAEVK
                     TTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQCERAEGRHSTGGMDELYKGGTGGS
                     LVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAFQTAKLKVTKGGPL
                     PFAWDILSPQFMYGSKAYIKHPADIPDYFKLSFPEGFRWERVMNFEDGGIIHVNQDSSL
                     QDGVFIYKVKLRGTNFPPDGPVMQKKTMGWEAAGVGFCFEAGADEDTHFRATSLETLRN
                     MVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGSPLRSRYEQLAEA
                     IRARMDGHFDKVLKQAV"
     primer_bind     3266..3285
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3667..4255
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3720..3740)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4138..4149)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4399..4432)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4452..4632
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4657..4675)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4658..4675)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4965..4984)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5198..5217)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5317..5339
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5377..5395)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5463..5567
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5568..5636,5637..6428)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5786..5805)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6599..7187
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7088..7107
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7341..7358
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7428..7448)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7433..7762
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7613..7748
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8896..8961
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9091..9111
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9536..9670
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9573..9592)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9627..9646
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9838..10471
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10518..10643
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11136..11369
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11554..11598
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaatttcgac atttaaattt
     1201 aattaatctc gacggtatcg gttaactttt aaaagaaaag gggggattgg ggggtacagt
     1261 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     1321 caaattacaa aaattcaaaa ttttatcgat cacgagacta gcctcgaggt ttaaacgcgg
     1381 atccgcgacg cgtgctagcc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     1441 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     1501 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tccaccaccg
     1561 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     1621 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     1681 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     1741 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     1801 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaactgc cacaacgtct
     1861 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     1921 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     1981 gccccgtgct gctgcccgac aaccactacc tgagcacctg ctccgccctg agcaaagacc
     2041 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     2101 tcggcatgga cgagctgtac aagggaagcg gagagggcag aggaagtctg ctaacatgcg
     2161 gtgacgtcga ggagaatcct ggacccatgg agatggcgag ccagcagggc gagacgatgt
     2221 ccggaccgct gcacattggt ttgattccca cagttggacc gtacctgcta ccgcatatta
     2281 tccctatgct gcaccagacc tttccaaagc tggaaatgta tctgcatgaa gcacagaccc
     2341 accagttact ggcgcaactg gacagcggca aactcgattg cgtgatcctc gcactggtga
     2401 aagagagcga agcattcatt gaagtgccgt tgtttgatga gccaatgttg ctggctatct
     2461 atgaagatca cccgtgggcg aaccgcgaat gcgtaccgat ggccgatctg gcaggggaaa
     2521 aactgctgat gctggaagat ggtcactgtt tgcgcgatca ggcaatgccc cgcacggttt
     2581 ccgagcggat gtaccccgag gacggcgccc tgaagagcga gatcaagaag gggctgaggc
     2641 tgaaggacgg cggccactac gccgccgagg tcaagaccac ctacaaggcc aagaagcccg
     2701 tgcagctgcc cggcgcctac atcgtcgaca tcaagttgga catcgtgtcc cacaacgagg
     2761 actacaccat cgtggaacag tgcgaacgcg ccgagggccg ccactccacc ggcggcatgg
     2821 acgagctgta caagggaggt acaggcggga gtctggtgag caagggcgag gaggataaca
     2881 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     2941 acgagttcga gatcgagggc gagggcgagg gccgccccta cgaggccttt cagaccgcta
     3001 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     3061 tcatgtacgg ctccaaggcc tacattaagc acccagccga catccccgac tacttcaagc
     3121 tgtccttccc cgagggcttc aggtgggagc gcgtgatgaa cttcgaggac ggcggcatta
     3181 ttcacgttaa ccaggactcc tccctgcagg acggcgtatt catctacaag gtgaagctgc
     3241 gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     3301 ctgccggggt gggtttctgt tttgaagccg gggcggatga agatacacac ttccgcgcga
     3361 ccagcctgga aactctgcgc aacatggtgg cggcaggtag cgggatcact ttactgccag
     3421 cgctggctgt gccgccggag cgcaaacgcg atggggttgt ttatctgccg tgcattaagc
     3481 cggaaccacg ccgcactatt ggtctggttt atcgtcctgg ctcaccgctg cgcagccgct
     3541 atgagcagct ggcagaggcc atccgcgcaa gaatggatgg ccatttcgat aaagttttaa
     3601 aacaggcggt ttaatgagaa ttctgcagtc gacggtaccg tacgtcgacc tcgagggaat
     3661 tccgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     3721 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     3781 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     3841 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     3901 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     3961 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     4021 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     4081 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     4141 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     4201 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcgg
     4261 gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     4321 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatgg
     4381 gatcaattca ccatgggaat aacttcgtat agcatacatt atacgaagtt atgctgcttt
     4441 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     4501 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     4561 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     4621 aaatctctag cagcatctag aattaattcc gtgtattcta tagtgtcacc taaatcgtat
     4681 gtgtatgata cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa
     4741 gcctaattgt gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc
     4801 cgtcagagtc ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc
     4861 ggaaatggtc agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg
     4921 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat
     4981 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg
     5041 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt
     5101 ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga
     5161 gtcgcataag ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat
     5221 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     5281 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     5341 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     5401 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     5461 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5521 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5581 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5641 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5701 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5761 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5821 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5881 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5941 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6001 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6061 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6121 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6181 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6241 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6301 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6361 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6421 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6481 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6541 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6601 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6661 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6721 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6781 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6841 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6901 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6961 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7021 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7081 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7141 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7201 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7261 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7321 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     7381 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca
     7441 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7501 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7561 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     7621 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7681 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7741 tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga
     7801 gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg
     7861 aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga
     7921 acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg
     7981 tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg
     8041 aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc
     8101 gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa
     8161 ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat
     8221 tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc
     8281 gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt
     8341 tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc
     8401 gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa
     8461 cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg
     8521 caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag
     8581 tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca
     8641 tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac
     8701 gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg
     8761 atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg
     8821 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
     8881 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
     8941 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
     9001 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
     9061 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
     9121 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
     9181 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
     9241 atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca taacatactg
     9301 ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg
     9361 tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc
     9421 ttgactagag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     9481 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     9541 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     9601 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     9661 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
     9721 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
     9781 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
     9841 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
     9901 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     9961 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
    10021 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
    10081 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
    10141 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
    10201 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
    10261 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
    10321 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
    10381 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
    10441 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
    10501 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
    10561 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
    10621 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
    10681 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
    10741 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
    10801 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
    10861 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
    10921 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
    10981 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
    11041 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
    11101 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
    11161 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
    11221 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
    11281 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
    11341 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
    11401 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
    11461 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
    11521 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
    11581 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
    11641 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
    11701 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
    11761 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
    11821 agatccattc gattagtgaa cggatctcga cggtatcgat cacgagacta gcctcgacca
    11881 tcgatgtcga agctttgcaa agatggataa agttttaaac agagaggaat ctttgcagct
    11941 aatggacctt ctaggtcttg aaaggagtgg gaatt
//
