LOCUS       sequence-331746-       12773 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant binary expression vector containing the intron and proximal 
            polyadenylation signal sequences of the Copia93 retroelement EVADE 
            (AT5G17125) between mGFP5 and GUS under CaMV 35S promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12773)
  AUTHORS   Oberlin S, Sarazin A, Chevalier C, Voinnet O, Mari-Ordonez A
  TITLE     A genome-wide transcriptome and translatome analysis of Arabidopsis 
            transposons identifies a unique and conserved genome expression 
            strategy for Ty1/Copia retroelements.
  JOURNAL   Genome Res. 2017 Sep;27(9):1549-1562. doi: 10.1101/gr.220723.117. 
            Epub 2017 Aug 7.
  PUBMED    28784835
REFERENCE   2  (bases 1 to 12773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.220723.117"; journalName: "Genome Res"; date: "2017-09";
            volume: "27"; issue: "9"; pages: "1549-1562"
COMMENT     Sequence Label: p35S_GFP-EVDinter-GUS
FEATURES             Location/Qualifiers
     source          1..12773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1..18
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        679..1023
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     967..987
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1124..1148
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(1179..1207)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1802..1822
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             2703..4514
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     protein_bind    complement(4515..4539)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4552..4561
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        4959..5138
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             5180..5731
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      5751..6003
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6096..6120
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6641..7432
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7678..8266
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8167..8186
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8420..8437
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8452..8592
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8518..8537)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8678..8700
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8936..9130
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9196..10269)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10698..11327)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12658..12682
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    12751..12771
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 atttaggtga cactatagaa tactcaagct atgcatccaa cgcgttggga gctctcccat
       61 atggtcgact agagccaagc tgatctcctt tgccccggag atcaccatgg acgactttct
      121 ctatctctac gatctaggaa gaaagttcga cggagaaggt gacgatacca tgttcaccac
      181 cgataatgag aagattagcc tcttcaattt cagaaagaat gctgacccac agatggttag
      241 agaggcctac gcggcaggtc tcatcaagac gatctacccg agtaataatc tccaggagat
      301 caaatacctt cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa
      361 gaacacagag aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca
      421 aggcttgctt cataaaccaa ggcaagtaat agagattgga gtctctaaga aagtagttcc
      481 tactgaatca aaggccatgg agtcaaaaat tcagatcgag gatctaacag aactcgccgt
      541 gaagactggc gaacagttca tacagagtct tttacgactc aatgacaaga agaaaatctt
      601 cgtcaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc
      661 agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg
      721 attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac
      781 ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgccc ctgccgacag
      841 tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac
      901 cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca
      961 atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag
     1021 gactccggta tttttacaac aattaccaca acaaaacaaa caacaaacaa cattacaatt
     1081 tactattcta gtcgacctgc aggcggccgc actagtgata tcagcaagtt tgtacaaaaa
     1141 agcaggctat gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
     1201 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
     1261 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
     1321 caaccctggt caccaccctg acctacggcg tgcagtgctt ctcccgttac cctgatcata
     1381 tgaagcggca cgacttcttc aagagcgcca tgcctgaggg atacgtgcag gagaggacca
     1441 tcttcttcaa ggacgacggg aactacaaga cacgtgctga agtcaagttt gagggagaca
     1501 ccctcgtcaa caggatcgag cttaagggaa tcgatttcaa ggaggacgga aacatcctcg
     1561 gccacaagtt ggaatacaac tacaactccc acaacgtata catcatggcc gacaagcaaa
     1621 agaacggcat caaagccaac ttcaagaccc gccacaacat cgaagacggc ggcgtgcaac
     1681 tcgctgatca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
     1741 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
     1801 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggta gaactataca
     1861 aactcgagga gacatccaca tcattcggag actatgtgag gaagtcggac ttggaagcac
     1921 tcatcaagtc cattgcttca ctcaaagaat ccggtatcac tttctcatca caaacatcta
     1981 gtagttcaat tgtgatagac tcgggtgctt ctcatcatat gattagtaac tctaatcttc
     2041 ttgataacat agaaccagct ctaggacatg ttattattgc taatggtgac aaagttccaa
     2101 ttgaagggat aggaaacttg aaattgttta acaaagattc aaaagctttc tttatgccta
     2161 agttcacatc taacttatta tccgtgaaaa ggaccactag agacttaaat tgttatgcga
     2221 tttttggccc taatgatgtg tattttcagg atattgagac gggaaaagtt attggagaag
     2281 gtggttcaaa aggagaactc tatgttcttg aagacttgtc accaaactct tctagctgtt
     2341 tttcctctaa gtcacattta ggcatctctt ttaatacttt gtggcatgct aggctaggtc
     2401 atcctcatac tagagctctt aaactcatgt taccaaatat ttcttttgat catacaagtt
     2461 gtgaggcttg tattcttggt aaacattgca aatcggtttt tcctaaatct cttactatct
     2521 atgagaaatg ttttgatcta gttcattcgg atgtttggac ctcaccatgt gtttctagag
     2581 acaacaacaa atattttgtc acatttattg atgagaaatc aaagtacact tggataacac
     2641 tattaccctc aaaagataga gtgtttgaag ctttcactaa ctttgaaacc tatgtcccta
     2701 ggatgttacg tcctgtagaa accccaaccc gtgaaatcaa aaaactcgac ggcctgtggg
     2761 cattcagtct ggatcgcgaa aactgtggaa ttgatcagcg ttggtgggaa agcgcgttac
     2821 aagaaagccg ggcaattgct gtgccaggca gttttaacga tcagttcgcc gatgcagata
     2881 ttcgtaatta tgcgggcaac gtctggtatc agcgcgaagt ctttataccg aaaggttggg
     2941 caggccagcg tatcgtgctg cgtttcgatg cggtcactca ttacggcaaa gtgtgggtca
     3001 ataatcagga agtgatggag catcagggcg gctatacgcc atttgaagcc gatgtcacgc
     3061 cgtatgttat tgccgggaaa agtgtacgta tcaccgtttg tgtgaacaac gaactgaact
     3121 ggcagactat cccgccggga atggtgatta ccgacgaaaa cggcaagaaa aagcagtctt
     3181 acttccatga tttctttaac tatgccggaa tccatcgcag cgtaatgctc tacaccacgc
     3241 cgaacacctg ggtggacgat atcaccgtgg tgacgcatgt cgcgcaagac tgtaaccacg
     3301 cgtctgttga ctggcaggtg gtggccaatg gtgatgtcag cgttgaactg cgtgatgcgg
     3361 atcaacaggt ggttgcaact ggacaaggca ctagcgggac tttgcaagtg gtgaatccgc
     3421 acctctggca accgggtgaa ggttatctct atgaactgtg cgtcacagcc aaaagccaga
     3481 cagagtgtga tatctacccg cttcgcgtcg gcatccggtc agtggcagtg aagggcgaac
     3541 agttcctgat taaccacaaa ccgttctact ttactggctt tggtcgtcat gaagatgcgg
     3601 acttgcgtgg caaaggattc gataacgtgc tgatggtgca cgaccacgca ttaatggact
     3661 ggattggggc caactcctac cgtacctcgc attaccctta cgctgaagag atgctcgact
     3721 gggcagatga acatggcatc gtggtgattg atgaaactgc tgctgtcggc tttaacctct
     3781 ctttaggcat tggtttcgaa gcgggcaaca agccgaaaga actgtacagc gaagaggcag
     3841 tcaacgggga aactcagcaa gcgcacttac aggcgattaa agagctgata gcgcgtgaca
     3901 aaaaccaccc aagcgtggtg atgtggagta ttgccaacga accggatacc cgtccgcaag
     3961 gtgcacggga atatttcgcg ccactggcgg aagcaacgcg taaactcgac ccgacgcgtc
     4021 cgatcacctg cgtcaatgta atgttctgcg acgctcacac cgataccatc agcgatctct
     4081 ttgatgtgct gtgcctgaac cgttattacg gatggtatgt ccaaagcggc gatttggaaa
     4141 cggcagagaa ggtactggaa aaagaacttc tggcctggca ggagaaactg catcagccga
     4201 ttatcatcac cgaatacggc gtggatacgt tagccgggct gcactcaatg tacaccgaca
     4261 tgtggagtga agagtatcag tgtgcatggc tggatatgta tcaccgcgtc tttgatcgcg
     4321 tcagcgccgt cgtcggtgaa caggtatgga atttcgccga ttttgcgacc tcgcaaggca
     4381 tattgcgcgt tggcggtaac aagaaaggga tcttcactcg cgaccgcaaa ccgaagtcgg
     4441 cggcttttct gctgcaaaaa cgctggactg gcatgaactt cggtgaaaaa ccgcagcagg
     4501 gaggcaaaca atgaacccag ctttcttgta caaagtggtg atatcccgcg gccatggcgg
     4561 ccgggagcat gcgacgtcga tctaactgac tagccgcggc catgctagag tccgcaaaaa
     4621 tcaccagtct ctctctacaa atctatctct ctctattttt ctccagaata atgtgtgagt
     4681 agttcccaga taagggaatt agggttctta tagggtttcg ctcatgtgtt gagcatataa
     4741 gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc
     4801 taaaaccaaa atccagtgac ctgcaggcat gcgacgtcgg gccctctaga ggatccccgg
     4861 gtaccgcgaa ttatcgatca tgagcggaga attaagggag tcacgttatg acccccgccg
     4921 atgacgcggg acaagccgtt ttacgtttgg aactgacaga accgcaacgt tgaaggagcc
     4981 actgagccgc gggtttctgg agtttaatga gctaagcaca tacgtcagaa accattattg
     5041 cgcgttcaaa agtcgcctaa ggtcactatc agctagcaaa tatttcttgt caaaaatgct
     5101 ccactgacgt tccataaatt cccctcggta tccaattaga gtctcatatt cactctcaac
     5161 tcgatcgagg ggatctacca tgagcccaga acgacgcccg gccgacatcc gccgtgccac
     5221 cgaggcggac atgccggcgg tctgcaccat cgtcaaccac tacatcgaga caagcacggt
     5281 caacttccgt accgagccgc aggaaccgca ggagtggacg gacgacctcg tccgtctgcg
     5341 ggagcgctat ccctggctcg tcgccgaggt ggacggcgag gtcgccggca tcgcctacgc
     5401 gggtccctgg aaggcacgca acgcctacga ctggacggcc gagtcgaccg tgtacgtctc
     5461 cccccgccac cagcggacgg gactgggctc cacgctctac acccacctgc tgaagtccct
     5521 ggaggcacag ggcttcaaga gcgtggtcgc tgtcatcggg ctgcccaacg acccgagcgt
     5581 gcgcatgcac gaggcgctcg gatatgcccc ccgcggcatg ctgcgggcgg ccggcttcaa
     5641 gcacgggaac tggcatgacg tgggtttctg gcagctggac ttcagcctgc cggtgccgcc
     5701 ccgtccggtc ctgcccgtca ccgaaatctg atgaccccta gagtcaagca gatcgttcaa
     5761 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
     5821 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
     5881 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
     5941 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
     6001 atcgaccggc atgcaagctg ataattcaat tcggcgttaa ttcagtacat taaaaacgtc
     6061 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
     6121 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
     6181 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
     6241 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
     6301 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaat
     6361 tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
     6421 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     6481 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
     6541 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     6601 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc
     6661 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     6721 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
     6781 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     6841 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     6901 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     6961 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     7021 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     7081 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     7141 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     7201 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     7261 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     7321 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     7381 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctagc
     7441 tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc gttagatgca
     7501 ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa
     7561 gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca aaatccctta
     7621 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7681 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7741 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7801 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     7861 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7921 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7981 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8041 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8101 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8161 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8221 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8281 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8341 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8401 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     8461 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     8521 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     8581 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     8641 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     8701 ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc
     8761 gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat
     8821 ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag
     8881 cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc
     8941 acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc
     9001 gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt
     9061 tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt
     9121 gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa tttgccctag
     9181 catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg
     9241 catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc
     9301 atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc
     9361 actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg
     9421 tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac
     9481 cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc
     9541 gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa
     9601 ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat
     9661 ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc
     9721 gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc
     9781 gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc
     9841 catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc
     9901 tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga
     9961 cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag
    10021 catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc
    10081 accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga
    10141 ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa
    10201 cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg
    10261 accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg ccggcagaca
    10321 acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca ttccacggcg
    10381 tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa aattcatcta
    10441 ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag atagcagctc
    10501 ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat cctccgccgg
    10561 caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca acgttgcagc
    10621 cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt tgctcatttt
    10681 ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag cgcctggacc
    10741 tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg
    10801 cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc gtacccggcc
    10861 agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag
    10921 gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc caccaggtcg
    10981 gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc
    11041 ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg
    11101 cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt
    11161 agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg
    11221 actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc
    11281 gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg ttccccttgc
    11341 gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc aagctgtttt
    11401 actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg gccaagctgg
    11461 ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc agccgcacgg
    11521 ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc gcctcgatct
    11581 cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc
    11641 ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa
    11701 ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta
    11761 cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc ggccttcctg
    11821 gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc gggggccaaa
    11881 cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga tgattaggga
    11941 acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt
    12001 ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct ggatgcgctc
    12061 ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac
    12121 aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt cgcgcctggt
    12181 gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt cggcccgttg
    12241 gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc cagcggcggc
    12301 gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtattctact ttatgcgact
    12361 aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta
    12421 ggacttgtgc gacatgtcgt tttcagaaga cggctgcact gaacgtcaga agccgactgc
    12481 actatagcag cggaggggtt ggatcaaagt actttaaagt actttaaagt actttaaagt
    12541 actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa
    12601 accttttcac gcccttttaa atatccgatt attctaataa acgctctttt ctcttaggtt
    12661 tacccgccaa tatatcctgt caaacactga tagtttaaac tgaaggcggg aaacgacaat
    12721 ctgatccaag ctcaagctaa gcttgagctc caactttgta tagaaaagtt gtc
//
