LOCUS       sequence-331750-       13515 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant binary expression vector containing the coding sequence of the
            Arabidopsis Copia93 retroelement EVADE (AT5G17125) under CaMV 35S 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13515)
  AUTHORS   Oberlin S, Sarazin A, Chevalier C, Voinnet O, Mari-Ordonez A
  TITLE     A genome-wide transcriptome and translatome analysis of Arabidopsis 
            transposons identifies a unique and conserved genome expression 
            strategy for Ty1/Copia retroelements.
  JOURNAL   Genome Res. 2017 Sep;27(9):1549-1562. doi: 10.1101/gr.220723.117. 
            Epub 2017 Aug 7.
  PUBMED    28784835
REFERENCE   2  (bases 1 to 13515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.220723.117"; journalName: "Genome Res"; date: "2017-09";
            volume: "27"; issue: "9"; pages: "1549-1562"
COMMENT     Sequence Label: p35S_EVDwt
FEATURES             Location/Qualifiers
     source          1..13515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        612..956
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     900..920
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1056..1080
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5299..5323)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5717..5896
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             5938..6489
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      6509..6761
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6854..6878
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7399..8190
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8436..9024
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8925..8944
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9178..9195
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9210..9350
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9276..9295)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9436..9458
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9694..9888
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9954..11027)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11456..12085)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13416..13440
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 actagagcca agctgatctc ctttgccccg gagatcacca tggacgactt tctctatctc
       61 tacgatctag gaagaaagtt cgacggagaa ggtgacgata ccatgttcac caccgataat
      121 gagaagatta gcctcttcaa tttcagaaag aatgctgacc cacagatggt tagagaggcc
      181 tacgcggcag gtctcatcaa gacgatctac ccgagtaata atctccagga gatcaaatac
      241 cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaactgcat caagaacaca
      301 gagaaagata tatttctcaa gatcagaagt actattccag tatggacgat tcaaggcttg
      361 cttcataaac caaggcaagt aatagagatt ggagtctcta agaaagtagt tcctactgaa
      421 tcaaaggcca tggagtcaaa aattcagatc gaggatctaa cagaactcgc cgtgaagact
      481 ggcgaacagt tcatacagag tcttttacga ctcaatgaca agaagaaaat cttcgtcaac
      541 atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac
      601 caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat
      661 tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa
      721 tgccatcatt gcgataaagg aaaggctatc gttcaagatg cccctgccga cagtggtccc
      781 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
      841 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac
      901 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gaggactccg
      961 gtatttttac aacaattacc acaacaaaac aaacaacaaa caacattaca atttactatt
     1021 ctagtcgacc tgcaggcggc cgcactagtg atatcacaag tttgtacaaa aaagcaggct
     1081 tcatggagac ttctcaaaag atgattacta cggtgatact tcaaggggga aactacctaa
     1141 cttggtcaag aacaacaaaa acggtgttgt gtggtcgtgg gctttggagc catgtaatct
     1201 catctaaagc tccaaaagaa gccaaagaag aagaagaaac ggagactata tctcccgagg
     1261 aagaaaaatg gttccaagag gatcaagctg tccttgctct tcttcaaaac tctcttgaaa
     1321 cctctattct agagggatac tcttactgtg agacagcaaa agagttgtgg gatacactta
     1381 aaaacgtgta tgggaacgaa tcgaatttga cccgcgtgtt tgaagtgaag aaggcgatta
     1441 acgaactaag tcaagaagac ttagagttta ctaaacactt tggtaagttc aggtccttat
     1501 ggtctgaact tgagagcttg aggccgggaa cgcttgaccc gaagattctc cacgaaaggc
     1561 gtgagcaaga caaggtgttt ggactccttc tcaccttaaa tccagggtac aatgacctaa
     1621 ttaaacactt gcttaggtcg gagaagttac caagtcttga tgaggtgtgt tctaagattc
     1681 agaaagagca aggatcaacc gggctatttg gtggaaaaag tgaactcatc acggctaaca
     1741 aaggagaagt agtggcgaac aaaggtgtgt acaagaatga ggatagaaaa ctgttgactt
     1801 gtgatcattg taagaagaag ggccacacca aagacaaatg ttggttgcta catccacacc
     1861 tcaagcccgc caagttcaaa gactcaagag ctcatctctc tcaagaaact catgaggagc
     1921 aaagtcaagc cgggtcatcg aaaggagaga catccacatc attcggagac tatgtgagga
     1981 agtcggactt ggaagcactc atcaagtcca ttgcttcact caaagaatcc ggtatcactt
     2041 tctcatcaca aacatctagt agttcaattg tgatagactc gggtgcttct catcatatga
     2101 ttagtaactc taatcttctt gataacatag aaccagctct aggacatgtt attattgcta
     2161 atggtgacaa agttccaatt gaagggatag gaaacttgaa attgtttaac aaagattcaa
     2221 aagctttctt tatgcctaag ttcacatcta acttattatc cgtgaaaagg accactagag
     2281 acttaaattg ttatgcgatt tttggcccta atgatgtgta ttttcaggat attgagacgg
     2341 gaaaagttat tggagaaggt ggttcaaaag gagaactcta tgttcttgaa gacttgtcac
     2401 caaactcttc tagctgtttt tcctctaagt cacatttagg catctctttt aatactttgt
     2461 ggcatgctag gctaggtcat cctcatacta gagctcttaa actcatgtta ccaaatattt
     2521 cttttgatca tacaagttgt gaggcttgta ttcttggtaa acattgcaaa tcggtttttc
     2581 ctaaatctct tactatctat gagaaatgtt ttgatctagt tcattcggat gtttggacct
     2641 caccatgtgt ttctagagac aacaacaaat attttgtcac atttattgat gagaaatcaa
     2701 agtacacttg gataacacta ttaccctcaa aagatagagt gtttgaagct ttcactaact
     2761 ttgaaaccta tgtcactaat caatttaatg caaagattaa agtgtttagg accgataatg
     2821 gaggagaata cacaagccaa aagtttcgag atcatctagc caaacgtgga ataatacacc
     2881 aaacaagttg cccgtacaca ccacaacaaa atggagtggc ggagagaaag aaccgacatc
     2941 tcatggaggt ggcgcggtca atgatgtttc atacaagtgt tccaaagaga ttttggggtg
     3001 atgcggtctt aaccgcgtgc taccttatca atcgaacacc aactaaagtc ttaagtgact
     3061 tatctccctt cgaggtactt aacaatatta aaccgttcat tgatcactta cgagtgtttg
     3121 gttgtgtatg ttttgttttg atacccggag aacaaagaag caagctagat gcgaagagca
     3181 ctaagtgtat gtttcttggc tactcaacta ctcaaaaagg atataaatgc tttgatccaa
     3241 ccaagaaccg cacatttata tcccgagatg tcaagtttct tgaaaaccaa gactacaaca
     3301 acaagaagga ttgggagaac ctaaaagatc ttactcattc cacctccgat cgtgttgcaa
     3361 ctctaaagtt tctattggat catttgggca atgactcaac ttcgacaact caacatcaac
     3421 cggagatgac acaagatcaa gaagacttga atcaagagaa tgaggaggtg tccctacaac
     3481 atcaagaaaa tcttacacac gtgcaagaag atcctccaaa cacacaagaa cactctgaac
     3541 atgttcaaga aatccaagat gactcaagtg aagatgaaga acgtacacaa gttcttccac
     3601 ctccaccacc tcttagaagg agtacacgca ttcgacgcaa gaaggaattc ttcaactcta
     3661 atgcggtagc tcatcctttc caagcgacat gttctcttgc ccttgtacct ctagatcatc
     3721 aagcgtttct ctccaagatt tctgagcatc ggattcctca aacatatgaa gaggcaatgg
     3781 aagtcaagga atggagggat gcgatagccg atgagataaa cgctatgaaa cgaaatcata
     3841 cttgggatga agatgactta cctaaaggaa agaaaacagt gagctcaagg tgggtattca
     3901 caatcaagta taagagtaat ggagatatcg agagatataa aactcgcttg gtggctcggg
     3961 ggttcacgca aacatatggc tccgactaca tggagacttt tgctccggtg gctaagctac
     4021 acacggtgag agtggttctt gccttagcaa caaatttatc atggggacta tggcaaatgg
     4081 atgtcaagaa tgcatttctc caaggcgaac ttgaagatga tgtgtatatg acgcctccac
     4141 ccggtctaga agataccatt ccatgtgata aagtgttgag actacgcaaa gctatctatg
     4201 gtcttaaaca atctccacgt gcatggtacc acaagctcag ccgaactctt aaagatcatg
     4261 gattcaagaa gtccgagtcc gatcacactt tgtttacatt acaaagtcct caaggtattg
     4321 tcgttgttct catctatgtt gatgatctta tcatcaccgg agacaacaaa gacggtatag
     4381 actctaccaa gacttttctt aaatcttgtt ttgatattaa agatcttggt gaactcaaat
     4441 acttcctagg aattgaagtg tgtcgctcta atgctggact ctttctatca caacgcaaat
     4501 acactcttga cttgttgaat gagacaggtt ttatggacgc taaaccggct cgcactccac
     4561 ttgaagatgg ctacaaggtc aatcgaaagg gggagaaaga agatgagaag tttggcgatg
     4621 cacctctcta tcgcaaactt gtaggtaagt tgatctattt gacaaacaca agacctgaca
     4681 tttgttttgc ggtaaatcag gttagtcaac acatgaaagt cccgatggtg tatcattgga
     4741 acatggtgga gagaatacta aggtatctca aaggcagctc cggacaaggc atatggatgg
     4801 gaaagaactc aagcaccgag atagtgggat attgcgatgc ggactatgcg ggtgatagag
     4861 gagatagaag atctacaact ggttattgta cgttcattgg aggtaacctt gcaacttgga
     4921 agacaaagaa acagaaggtg gtctcatgtt cgagtgcaga atcggagtat agagcaatga
     4981 gaaagctcac caacgaactc acttggctta aagctcttct taaagatgtt ggcattgaac
     5041 aacacatgcc tatcaccatg cattgtgaca acaaggcagc aatatacata gcctcaaatt
     5101 cggtgtttca tgagagaacc aagcatattg aagtcgattg tcataaagtc cgagagaaga
     5161 tcatcgaagg agtcacacta ccgtgttata caagaagtga agatcagttg gcggatatct
     5221 tcaccaaagc cgcaagtctc aaagtttgca acttcataca tggcaaatta ggacttgtgg
     5281 atctatctca ctcttgaaac ccagctttct tgtacaaagt ggtgatatcc cgcggccatg
     5341 ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc
     5401 agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca
     5461 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
     5521 taaaatttct aattcctaaa accaaaatcc agtgacctgc aggcatgcga cgtcgagctt
     5581 gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg agctcgaatt atcgatcatg
     5641 agcggagaat taagggagtc acgttatgac ccccgccgat gacgcgggac aagccgtttt
     5701 acgtttggaa ctgacagaac cgcaacgttg aaggagccac tgagccgcgg gtttctggag
     5761 tttaatgagc taagcacata cgtcagaaac cattattgcg cgttcaaaag tcgcctaagg
     5821 tcactatcag ctagcaaata tttcttgtca aaaatgctcc actgacgttc cataaattcc
     5881 cctcggtatc caattagagt ctcatattca ctctcaactc gatcgagggg atctaccatg
     5941 agcccagaac gacgcccggc cgacatccgc cgtgccaccg aggcggacat gccggcggtc
     6001 tgcaccatcg tcaaccacta catcgagaca agcacggtca acttccgtac cgagccgcag
     6061 gaaccgcagg agtggacgga cgacctcgtc cgtctgcggg agcgctatcc ctggctcgtc
     6121 gccgaggtgg acggcgaggt cgccggcatc gcctacgcgg gtccctggaa ggcacgcaac
     6181 gcctacgact ggacggccga gtcgaccgtg tacgtctccc cccgccacca gcggacggga
     6241 ctgggctcca cgctctacac ccacctgctg aagtccctgg aggcacaggg cttcaagagc
     6301 gtggtcgctg tcatcgggct gcccaacgac ccgagcgtgc gcatgcacga ggcgctcgga
     6361 tatgcccccc gcggcatgct gcgggcggcc ggcttcaagc acgggaactg gcatgacgtg
     6421 ggtttctggc agctggactt cagcctgccg gtgccgcccc gtccggtcct gcccgtcacc
     6481 gaaatctgat gacccctaga gtcaagcaga tcgttcaaac atttggcaat aaagtttctt
     6541 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     6601 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     6661 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     6721 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgaccggcat gcaagctgat
     6781 aattcaattc ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc
     6841 taagcgtcaa tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg
     6901 accggcagct cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg
     6961 tcagcgggag agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa
     7021 gaacggcaac taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg
     7081 attgtaacga tgacagagcg ttgctgcctg tgatcaattc gggcacgaac ccagtggaca
     7141 taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc
     7201 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
     7261 gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
     7321 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
     7381 aaaacaaagt taaacatcat gggggaagcg gtgatcgccg aagtatcgac tcaactatca
     7441 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     7501 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
     7561 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     7621 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     7681 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     7741 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     7801 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     7861 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     7921 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     7981 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     8041 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     8101 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa
     8161 ggcgagatca ccaaggtagt cggcaaataa tgtctagcta gaaattcgtt caagccgacg
     8221 ccgcttcgcg gcgcggctta actcaagcgt tagatgcact aagcacataa ttgctcacag
     8281 ccaaactatc aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac
     8341 aaattgggag atatatcatg catgaccaaa atcccttaac gtgagttttc gttccactga
     8401 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     8461 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     8521 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     8581 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     8641 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     8701 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     8761 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     8821 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     8881 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     8941 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     9001 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     9061 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     9121 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     9181 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
     9241 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     9301 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
     9361 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     9421 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     9481 gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt
     9541 ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc agcggccgcg
     9601 ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt aggcgctttt
     9661 tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg ggttttaaga
     9721 gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc ttttatatca
     9781 gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat gtacgggttc
     9841 cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg aaagagacct
     9901 tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt acattaggaa
     9961 ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat cgggccagcc
    10021 tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc
    10081 acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta gatcgtcttg
    10141 aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg
    10201 ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg
    10261 ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta ctccggccgc
    10321 ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc
    10381 accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac
    10441 cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg ccggcagaac
    10501 ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc cttcccttcc
    10561 cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc
    10621 cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg
    10681 tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc
    10741 atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa aaaatctggt
    10801 tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg cggttgccgg
    10861 gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg tgcttctgcc
    10921 tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag gtcatcaccc
    10981 agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgctcacgcc gattcctcgg
    11041 gcttgggggt tccagtgcca ttgcagggcc ggcagacaac ccagccgctt acgcctggcc
    11101 aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt tgttcttgat
    11161 tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca tttgctcatt
    11221 tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt gccttggcgt
    11281 accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt gacccgcttc
    11341 atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg tgcgctcgga
    11401 cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca ttaactcaaa
    11461 tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg tcgccctcgg
    11521 gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg
    11581 tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca acctcaccgc
    11641 cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg
    11701 tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat atgtcgtaag
    11761 ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac acagccaagt
    11821 ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt
    11881 cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct tcccgcacgg
    11941 ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca tcggccccgg
    12001 cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac ccgcctttct
    12061 ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg
    12121 catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac atcacctttt
    12181 tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg ccagcttggc
    12241 atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc
    12301 gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga aaaacggttc
    12361 gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc
    12421 gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg cctggcctcg
    12481 gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca
    12541 agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc
    12601 gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg ggccttggcg
    12661 gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc ggcaatgccg
    12721 gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc
    12781 aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg
    12841 gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg
    12901 tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac
    12961 agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg
    13021 gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt
    13081 ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac aagaaaacgc
    13141 caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga catgtcgttt
    13201 tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg
    13261 atcaaagtac tttaaagtac tttaaagtac tttaaagtac tttgatcccg aggggaaccc
    13321 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
    13381 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
    13441 aacactgata gtttaaactg aaggcgggaa acgacaatct gatccaagct caagctaagc
    13501 tctcccatat ggtcg
//
