LOCUS       sequence-331755-       13329 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant binary expression vector containing the coding sequence of the
            Arabidopsis Copia93 retroelement EVADE (AT5G17125) lacking the 
            intron under CaMV 35S promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13329)
  AUTHORS   Oberlin S, Sarazin A, Chevalier C, Voinnet O, Mari-Ordonez A
  TITLE     A genome-wide transcriptome and translatome analysis of Arabidopsis 
            transposons identifies a unique and conserved genome expression 
            strategy for Ty1/Copia retroelements.
  JOURNAL   Genome Res. 2017 Sep;27(9):1549-1562. doi: 10.1101/gr.220723.117. 
            Epub 2017 Aug 7.
  PUBMED    28784835
REFERENCE   2  (bases 1 to 13329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.220723.117"; journalName: "Genome Res"; date: "2017-09";
            volume: "27"; issue: "9"; pages: "1549-1562"
COMMENT     Sequence Label: p35S_EVDdi
FEATURES             Location/Qualifiers
     source          1..13329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1..18
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        679..1023
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     967..987
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1124..1148
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5071..5095)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5108..5117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        5515..5694
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             5736..6287
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      6307..6559
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6652..6676
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7197..7988
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8234..8822
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8723..8742
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8976..8993
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9008..9148
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9074..9093)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9234..9256
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9492..9686
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9752..10825)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11254..11883)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13214..13238
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    13307..13327
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 atttaggtga cactatagaa tactcaagct atgcatccaa cgcgttggga gctctcccat
       61 atggtcgact agagccaagc tgatctcctt tgccccggag atcaccatgg acgactttct
      121 ctatctctac gatctaggaa gaaagttcga cggagaaggt gacgatacca tgttcaccac
      181 cgataatgag aagattagcc tcttcaattt cagaaagaat gctgacccac agatggttag
      241 agaggcctac gcggcaggtc tcatcaagac gatctacccg agtaataatc tccaggagat
      301 caaatacctt cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa
      361 gaacacagag aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca
      421 aggcttgctt cataaaccaa ggcaagtaat agagattgga gtctctaaga aagtagttcc
      481 tactgaatca aaggccatgg agtcaaaaat tcagatcgag gatctaacag aactcgccgt
      541 gaagactggc gaacagttca tacagagtct tttacgactc aatgacaaga agaaaatctt
      601 cgtcaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc
      661 agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg
      721 attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac
      781 ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgccc ctgccgacag
      841 tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac
      901 cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca
      961 atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag
     1021 gactccggta tttttacaac aattaccaca acaaaacaaa caacaaacaa cattacaatt
     1081 tactattcta gtcgacctgc aggcggccgc actagtgata tcagcaagtt tgtacaaaaa
     1141 agcaggcttc atggagactt ctcaaaagat gattactacg gtgatacttc aagggggaaa
     1201 ctacctaact tggtcaagaa caacaaaaac ggtgttgtgt ggtcgtgggc tttggagcca
     1261 tgtaatctca tctaaagctc caaaagaagc caaagaagaa gaagaaacgg agactatatc
     1321 tcccgaggaa gaaaaatggt tccaagagga tcaagctgtc cttgctcttc ttcaaaactc
     1381 tcttgaaacc tctattctag agggatactc ttactgtgag acagcaaaag agttgtggga
     1441 tacacttaaa aacgtgtatg ggaacgaatc gaatttgacc cgcgtgtttg aagtgaagaa
     1501 ggcgattaac gaactaagtc aagaagactt agagtttact aaacactttg gtaagttcag
     1561 gtccttatgg tctgaacttg agagcttgag gccgggaacg cttgacccga agattctcca
     1621 cgaaaggcgt gagcaagaca aggtgtttgg actccttctc accttaaatc cagggtacaa
     1681 tgacctaatt aaacacttgc ttaggtcgga gaagttacca agtcttgatg aggtgtgttc
     1741 taagattcag aaagagcaag gatcaaccgg gctatttggt ggaaaaagtg aactcatcac
     1801 ggctaacaaa ggagaagtag tggcgaacaa aggtgtgtac aagaatgagg atagaaaact
     1861 gttgacttgt gatcattgta agaagaaggg ccacaccaaa gacaaatgtt ggttgctaca
     1921 tccacacctc aagcccgcca agttcaaaga ctcaagagct catctctctc aagaaactca
     1981 tgaggagcaa agtcaagccg ggtcatcgaa aggagagaca tccacatcat tcggagacta
     2041 tgtgaggaag tcggacttgg aagcactcat caagtccatt gcttcactca aagaatccgg
     2101 atattgagac gggaaaagtt attggagaag gtggttcaaa aggagaactc tatgttcttg
     2161 aagacttgtc accaaactct tctagctgtt tttcctctaa gtcacattta ggcatctctt
     2221 ttaatacttt gtggcatgct aggctaggtc atcctcatac tagagctctt aaactcatgt
     2281 taccaaatat ttcttttgat catacaagtt gtgaggcttg tattcttggt aaacattgca
     2341 aatcggtttt tcctaaatct cttactatct atgagaaatg ttttgatcta gttcattcgg
     2401 atgtttggac ctcaccatgt gtttctagag acaacaacaa atattttgtc acatttattg
     2461 atgagaaatc aaagtacact tggataacac tattaccctc aaaagataga gtgtttgaag
     2521 ctttcactaa ctttgaaacc tatgtcacta atcaatttaa tgcaaagatt aaagtgttta
     2581 ggaccgataa tggaggagaa tacacaagcc aaaagtttcg agatcatcta gccaaacgtg
     2641 gaataataca ccaaacaagt tgcccgtaca caccacaaca aaatggagtg gcggagagaa
     2701 agaaccgaca tctcatggag gtggcgcggt caatgatgtt tcatacaagt gttccaaaga
     2761 gattttgggg tgatgcggtc ttaaccgcgt gctaccttat caatcgaaca ccaactaaag
     2821 tcttaagtga cttatctccc ttcgaggtac ttaacaatat taaaccgttc attgatcact
     2881 tacgagtgtt tggttgtgta tgttttgttt tgatacccgg agaacaaaga agcaagctag
     2941 atgcgaagag cactaagtgt atgtttcttg gctactcaac tactcaaaaa ggatataaat
     3001 gctttgatcc aaccaagaac cgcacattta tatcccgaga tgtcaagttt cttgaaaacc
     3061 aagactacaa caacaagaag gattgggaga acctaaaaga tcttactcat tccacctccg
     3121 atcgtgttgc aactctaaag tttctattgg atcatttggg caatgactca acttcgacaa
     3181 ctcaacatca accggagatg acacaagatc aagaagactt gaatcaagag aatgaggagg
     3241 tgtccctaca acatcaagaa aatcttacac acgtgcaaga agatcctcca aacacacaag
     3301 aacactctga acatgttcaa gaaatccaag atgactcaag tgaagatgaa gaacgtacac
     3361 aagttcttcc acctccacca cctcttagaa ggagtacacg cattcgacgc aagaaggaat
     3421 tcttcaactc taatgcggta gctcatcctt tccaagcgac atgttctctt gcccttgtac
     3481 ctctagatca tcaagcgttt ctctccaaga tttctgagca tcggattcct caaacatatg
     3541 aagaggcaat ggaagtcaag gaatggaggg atgcgatagc cgatgagata aacgctatga
     3601 aacgaaatca tacttgggat gaagatgact tacctaaagg aaagaaaaca gtgagctcaa
     3661 ggtgggtatt cacaatcaag tataagagta atggagatat cgagagatat aaaactcgct
     3721 tggtggctcg ggggttcacg caaacatatg gctccgacta catggagact tttgctccgg
     3781 tggctaagct acacacggtg agagtggttc ttgccttagc aacaaattta tcatggggac
     3841 tatggcaaat ggatgtcaag aatgcatttc tccaaggcga acttgaagat gatgtgtata
     3901 tgacgcctcc acccggtcta gaagatacca ttccatgtga taaagtgttg agactacgca
     3961 aagctatcta tggtcttaaa caatctccac gtgcatggta ccacaagctc agccgaactc
     4021 ttaaagatca tggattcaag aagtccgagt ccgatcacac tttgtttaca ttacaaagtc
     4081 ctcaaggtat tgtcgttgtt ctcatctatg ttgatgatct tatcatcacc ggagacaaca
     4141 aagacggtat agactctacc aagacttttc ttaaatcttg ttttgatatt aaagatcttg
     4201 gtgaactcaa atacttccta ggaattgaag tgtgtcgctc taatgctgga ctctttctat
     4261 cacaacgcaa atacactctt gacttgttga atgagacagg ttttatggac gctaaaccgg
     4321 ctcgcactcc acttgaagat ggctacaagg tcaatcgaaa gggggagaaa gaagatgaga
     4381 agtttggcga tgcacctctc tatcgcaaac ttgtaggtaa gttgatctat ttgacaaaca
     4441 caagacctga catttgtttt gcggtaaatc aggttagtca acacatgaaa gtcccgatgg
     4501 tgtatcattg gaacatggtg gagagaatac taaggtatct caaaggcagc tccggacaag
     4561 gcatatggat gggaaagaac tcaagcaccg agatagtggg atattgcgat gcggactatg
     4621 cgggtgatag aggagataga agatctacaa ctggttattg tacgttcatt ggaggtaacc
     4681 ttgcaacttg gaagacaaag aaacagaagg tggtctcatg ttcgagtgca gaatcggagt
     4741 atagagcaat gagaaagctc accaacgaac tcacttggct taaagctctt cttaaagatg
     4801 ttggcattga acaacacatg cctatcacca tgcattgtga caacaaggca gcaatataca
     4861 tagcctcaaa ttcggtgttt catgagagaa ccaagcatat tgaagtcgat tgtcataaag
     4921 tccgagagaa gatcatcgaa ggagtcacac taccgtgtta tacaagaagt gaagatcagt
     4981 tggcggatat cttcaccaaa gccgcaagtc tcaaagtttg caacttcata catggcaaat
     5041 taggacttgt ggatctatct cactcttgaa acccagcttt cttgtacaaa gtggtgatat
     5101 cccgcggcca tggcggccgg gagcatgcga cgtcgatcta actgactagc cgcggccatg
     5161 ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc
     5221 agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca
     5281 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
     5341 taaaatttct aattcctaaa accaaaatcc agtgacctgc aggcatgcga cgtcgggccc
     5401 tctagaggat ccccgggtac cgcgaattat cgatcatgag cggagaatta agggagtcac
     5461 gttatgaccc ccgccgatga cgcgggacaa gccgttttac gtttggaact gacagaaccg
     5521 caacgttgaa ggagccactg agccgcgggt ttctggagtt taatgagcta agcacatacg
     5581 tcagaaacca ttattgcgcg ttcaaaagtc gcctaaggtc actatcagct agcaaatatt
     5641 tcttgtcaaa aatgctccac tgacgttcca taaattcccc tcggtatcca attagagtct
     5701 catattcact ctcaactcga tcgaggggat ctaccatgag cccagaacga cgcccggccg
     5761 acatccgccg tgccaccgag gcggacatgc cggcggtctg caccatcgtc aaccactaca
     5821 tcgagacaag cacggtcaac ttccgtaccg agccgcagga accgcaggag tggacggacg
     5881 acctcgtccg tctgcgggag cgctatccct ggctcgtcgc cgaggtggac ggcgaggtcg
     5941 ccggcatcgc ctacgcgggt ccctggaagg cacgcaacgc ctacgactgg acggccgagt
     6001 cgaccgtgta cgtctccccc cgccaccagc ggacgggact gggctccacg ctctacaccc
     6061 acctgctgaa gtccctggag gcacagggct tcaagagcgt ggtcgctgtc atcgggctgc
     6121 ccaacgaccc gagcgtgcgc atgcacgagg cgctcggata tgccccccgc ggcatgctgc
     6181 gggcggccgg cttcaagcac gggaactggc atgacgtggg tttctggcag ctggacttca
     6241 gcctgccggt gccgccccgt ccggtcctgc ccgtcaccga aatctgatga cccctagagt
     6301 caagcagatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     6361 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     6421 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     6481 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     6541 tcatctatgt tactagatcg accggcatgc aagctgataa ttcaattcgg cgttaattca
     6601 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
     6661 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
     6721 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
     6781 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
     6841 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
     6901 gctgcctgtg atcaattcgg gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     6961 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     7021 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
     7081 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     7141 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg
     7201 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     7261 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     7321 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     7381 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     7441 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     7501 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     7561 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     7621 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     7681 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     7741 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     7801 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     7861 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     7921 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     7981 gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc gcggcttaac
     8041 tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg
     8101 cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgca
     8161 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8221 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8281 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8341 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     8401 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8461 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8521 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8581 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8641 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8701 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8761 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8821 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8881 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8941 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     9001 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     9061 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     9121 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     9181 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     9241 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat
     9301 gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg
     9361 gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg
     9421 ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct
     9481 ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt
     9541 tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt
     9601 tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg
     9661 ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta
     9721 gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga
     9781 tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat
     9841 cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga
     9901 actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct
     9961 tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa
    10021 agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca
    10081 tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct
    10141 tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct
    10201 tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt
    10261 cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg
    10321 gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg
    10381 ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg
    10441 gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc
    10501 gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg
    10561 tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct
    10621 tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag
    10681 cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg
    10741 cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg
    10801 ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt
    10861 gcagggccgg cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat
    10921 ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc
    10981 cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt
    11041 attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg
    11101 tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc
    11161 tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg
    11221 tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc
    11281 ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt
    11341 gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg
    11401 cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc
    11461 ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac
    11521 cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag
    11581 cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc
    11641 gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc
    11701 gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc
    11761 ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag
    11821 atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt
    11881 catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca
    11941 tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct
    12001 tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt
    12061 tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc
    12121 atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt
    12181 ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca
    12241 atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc
    12301 gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca
    12361 cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg
    12421 tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc
    12481 ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc
    12541 ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg
    12601 cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta
    12661 gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg
    12721 ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt
    12781 gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca
    12841 gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat
    12901 tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag
    12961 cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac
    13021 gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt taaagtactt
    13081 taaagtactt taaagtactt tgatcccgag gggaaccctg tggttggcat gcacatacaa
    13141 atggacgaac ggataaacct tttcacgccc ttttaaatat ccgattattc taataaacgc
    13201 tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaactgaa
    13261 ggcgggaaac gacaatctga tccaagctca agctaagctt gagctccaac tttgtataga
    13321 aaagttgtc
//
