LOCUS       sequence-331771-       11295 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TagBFP-tagged Src kinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11295)
  AUTHORS   Dine E, Reed EH, Toettcher JE
  TITLE     Positive feedback between the T cell kinase Zap70 and its substrate 
            LAT acts as a clustering-dependent signaling switch.
  JOURNAL   Cell Rep. 2021 Jun 22;35(12):109280. doi: 
            10.1016/j.celrep.2021.109280.
  PUBMED    34161759
REFERENCE   2  (bases 1 to 11295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-06-22"; volume: "35(12):109280. doi"; pages: " 
            10.1016/j.celrep.2021.109280"
COMMENT     Sequence Label: pHR Src-TagBFP
FEATURES             Location/Qualifiers
     source          1..11295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             249..293
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    576..693
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        847..1255
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             2941..3639
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    3737..4325
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3790..3810)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4208..4219)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4388..4621
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4646..4664)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4647..4664)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4954..4973)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     5307..5329
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5367..5385)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5453..5557
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5558..5626,5627..6418)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5776..5795)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6589..7177
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7078..7097
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7331..7348
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7418..7438)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7423..7752
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7603..7738
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8886..8951
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9081..9101
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9526..9660
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9563..9582)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9617..9636
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9829..10462
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10509..10634
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    join(11126..11295,1..64)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
       61 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
      121 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
      181 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
      241 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
      301 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
      361 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
      421 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
      481 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
      541 ctcgacggtc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
      601 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
      661 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
      721 ccagtttgga tcgataagct tgatatcgaa ttcctgcagc cccgataaaa taaaagattt
      781 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggtta tggcaagcta
      841 gctgcagtaa cgccattatt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt
      901 cagatcaagg gcgggtacat gaaaatagct aacgtagggc caaacaggat atctgcggtg
      961 agcagtttcg gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc
     1021 ccgaggccaa gagcagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc
     1081 catcagatgt ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta
     1141 accaatcagc ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag
     1201 agctcacaac ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccggggggga
     1261 tctggagctc tcgagaattc tcacgcgtca agtggagcaa ggcaggtgga cagtggatcc
     1321 atgggcagca acaagagcaa gcccaaggac gccagccagc ggcgccgcag cctggagccc
     1381 tcggaaaacg tgcacggggc agggggcgcc ttcccggcct cacagacacc gagcaagccc
     1441 gcctccgccg acggccaccg cgggcccagc gccgccttcg tgccgcccgc ggccgagccc
     1501 aagctcttcg gaggcttcaa ctcctcggac accgtcacct ccccgcagag ggcggggcct
     1561 ctggcaggtg gggtgaccac ctttgtggcc ctctatgact atgagtcacg gacagagact
     1621 gacctgtcct tcaagaaagg ggagcggctg cagattgtca ataacacgga gggagactgg
     1681 tggctggcac actcgctgag cacgggacag accggttaca tccccagcaa ctatgtggcg
     1741 ccctccgact ccatccaggc tgaggagtgg tactttggca agatcactag acgggaatca
     1801 gagcggctgc tgctcaacgc cgagaacccg agagggacct tcctcgtgag ggagagtgag
     1861 accacaaaag gtgcctactg cctctctgta tccgacttcg acaatgccaa gggcctaaat
     1921 gtgaaacact acaagatccg caagctggac agcggcggtt tctacatcac ctcccgcacc
     1981 cagttcaaca gcctgcagca gctcgtggct tactactcca aacatgctga tggcctgtgt
     2041 caccgcctca ctaccgtatg tcccacatcc aagcctcaga cccagggatt ggccaaggat
     2101 gcgtgggaga tcccccggga gtccctgcgg ctggaggtca agctgggcca gggttgcttc
     2161 ggagaggtgt ggatggggac ctggaacggc accacgaggg ttgccatcaa gactctgaag
     2221 ccaggcacca tgtccccaga ggccttcctg caggaggccc aagtcatgaa gaaactgagg
     2281 cacgagaaac tggtgcagct gtatgctgtg gtgtcggaag aacccattta cattgtgaca
     2341 gagtacatga acaaggggag tctgctggac tttctcaagg gggaaacggg caaatatttg
     2401 cggctacccc agctggtgga catgtctgct cagatcgctt caggcatggc ctatgtggag
     2461 cggatgaact atgtgcaccg ggaccttcga gccgccaata tcctagtagg ggagaacctg
     2521 gtgtgcaaag tggccgactt tgggttggcc cggctcatag aagacaacga atacacagcc
     2581 cggcaaggtg ccaaattccc catcaagtgg accgcccctg aagctgctct gtacggcagg
     2641 ttcaccatca agtcggatgt gtggtccttt gggattctgc tgaccgagct caccactaag
     2701 ggaagagtgc cctatcctgg gatggtgaac cgtgaggttc tggaccaggt ggagcggggc
     2761 taccggatgc cttgtccccc cgagtgcccc gagtccctgc atgaccttat gtgccagtgc
     2821 tggcggaagg agcccgagga gcggcccacc ttcgagtacc tgcaggcctt cctggaagac
     2881 tactttacgt ccactgagcc acagtaccag cccggggaga acctaggatc cggagctacc
     2941 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
     3001 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     3061 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
     3121 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
     3181 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     3241 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     3301 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
     3361 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
     3421 gccctgaagc tcgtgggcgg gagccatctg atcgcaaaca tcaagaccac atatagatcc
     3481 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
     3541 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
     3601 tactgcgacc tccctagcaa actggggcac aagcttaatg cggccgcaac tcccacctgc
     3661 aacatgcgtg actgactgag gccgcgactc tagagtcgac ctgcaggcat gcaagcttga
     3721 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3781 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3841 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     3901 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     3961 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     4021 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     4081 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     4141 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     4201 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     4261 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     4321 cccgcatcga taccgtcgac caaggcagct gtagatctta gccacttttt aaaagaaaag
     4381 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     4441 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     4501 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     4561 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     4621 agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac
     4681 ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg
     4741 tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg
     4801 gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca
     4861 gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg
     4921 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
     4981 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
     5041 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
     5101 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
     5161 gagagcgtcg aatggtgcac tctcagtaca atctagctct gatgccgcat agttaagcca
     5221 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     5281 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     5341 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     5401 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     5461 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5521 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     5581 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5641 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     5701 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5761 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     5821 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     5881 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     5941 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6001 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     6061 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     6121 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6181 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6241 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6301 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6361 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6421 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6481 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6541 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6601 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6661 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6721 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6781 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6841 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6901 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6961 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7021 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7081 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7141 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7201 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7261 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     7321 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     7381 tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt
     7441 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     7501 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7561 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     7621 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7681 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7741 ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact
     7801 ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt
     7861 ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta
     7921 agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga
     7981 catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa
     8041 aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg
     8101 ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag
     8161 cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg
     8221 gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc
     8281 accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg
     8341 caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc
     8401 ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg
     8461 gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag
     8521 cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt
     8581 taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg
     8641 gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct
     8701 cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga
     8761 tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga
     8821 aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct
     8881 ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt
     8941 gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta
     9001 atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg
     9061 actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc
     9121 cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct
     9181 ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat
     9241 attctgtaac ctttataagt aggcataaca gttataatca taacatactg ttttttctta
     9301 ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta
     9361 gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag
     9421 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     9481 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     9541 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     9601 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     9661 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
     9721 actgccaatt acctagtggt ttcatttact ctaaacctgt gattcctctg aattattttc
     9781 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa
     9841 ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     9901 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
     9961 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
    10021 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
    10081 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
    10141 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
    10201 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
    10261 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
    10321 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
    10381 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
    10441 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
    10501 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
    10561 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
    10621 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
    10681 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
    10741 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
    10801 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
    10861 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
    10921 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggtg
    10981 atcttcagac ctggacgata tatatgaggg acaattggag aagtgaatta tataaatata
    11041 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
    11101 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
    11161 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
    11221 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
    11281 aactcacagt ctggg
//
