LOCUS       sequence-33178-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Lee SJ, Escobedo-Lozoya Y, Szatmari EM, Yasuda R
  TITLE     Activation of CaMKII in single dendritic spines during long-term 
            potentiation.
  JOURNAL   Nature. 2009 Mar 19. 458(7236):299-304.
  PUBMED    19295602
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Mar 19. 458(7236):299-304."
COMMENT     Sequence Label: Green-Camuialpha
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    125..246
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(136..155)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     190..209
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(372..623)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(414..435)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     763..782
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
       61 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttac
      121 gccttaagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
      181 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
      241 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
      301 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tctagagtcg
      361 cggccgcttt acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
      421 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagctt ggactgggtg
      481 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
      541 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc
      601 ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac
      661 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
      721 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
      781 tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
      841 tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccg
//
