LOCUS       sequence-33179-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Lee SJ, Escobedo-Lozoya Y, Szatmari EM, Yasuda R
  TITLE     Activation of CaMKII in single dendritic spines during long-term 
            potentiation.
  JOURNAL   Nature. 2009 Mar 19. 458(7236):299-304.
  PUBMED    19295602
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Mar 19. 458(7236):299-304."
COMMENT     Sequence Label: Green-Camuialpha
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      49..58
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(55..57,58..60,61..771)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNWNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(100..121)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(361..380)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     708..729
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttagcagagc tggttngtga accgtcagat ccgctagcgc taccggtcgc caccatggtg
       61 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      121 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      181 ctgaccctga agctgatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      241 accaccctgg gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
      301 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
      361 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      421 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      481 gagtacaact ggaacagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc
      541 aaggccaact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac
      601 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
      661 agctaccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
      721 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggactc
      781 agatctcgag ctatggctac catcacctgc acccgattca cggaagagta ccagctcttc
      841 gaggaactgg gaaagggagc cttctccgtg gtgcgca
//
