LOCUS       sequence-33181-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  TITLE     Victoria Prince lab plasmids
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBS ZfHoxa5a
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(778..798)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(778..796)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(817..833)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(817..833)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(830..852)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    841..857
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(865..871,872..889,890..895))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 gtcgacggta tcgataagct tgatatcgaa ttcctgcagc ccgggggatc catgcattcg
       61 ggcaggtatg cttgtggcta caatggaatg gatctcagca ccgggcactc cagccccgga
      121 cactttcttt catctggtga gcgaactcaa agctacaaag acagtccaac tgcgacgcct
      181 gtccggtaca atcagccggt taccgctagc agcgctgaac cttcctctga tcatctcccc
      241 tgctcatcct tagccaactc tcctgtcagt gagcagtcac accgcgccct gaaaatctca
      301 ctatctagca ccgctggctc tgcgtccaaa agctttggaa cggttctcag ccgggaggga
      361 gtttcgaaag tgtcaagctc tatggaggaa gaaaagccac cgggtagtgg acagacagct
      421 tcacagaatg tgtcggaggc accgcagatt tatccatgga tgcgaaaact tcatataagt
      481 catgataatt tggccggacc ggaggggaaa aggcccagga cagcctacac gcgctaccag
      541 acacttgagc tcgagaagga gttccacttc aaccgatacc tgacacgtcg ccgaagaatt
      601 gagattgcac acactctttg tctctcagag agacaaatca aaatatggtt tcaaaaccgc
      661 agaatgaaat ggaaaaaaga caacaaactg aaaagtatga atatggcagc tgcaggaagc
      721 ggataccggc cttaatctag agcggccgcc accgcggtgg agctccagct tttgttccct
      781 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
      841 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
      901 gggtgcctaa tgagtgagct aactcac
//
