LOCUS       sequence-331811-        1101 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp 
            construct for ampilifying the immobilized template for assays. 
            Originally came from Michael Carey, was submitted with his 
            permission.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   He Y, Sato S, Tomomori-Sato C, Chen S, Goode ZH, Conaway JW, Conaway
            RC
  TITLE     Elongin functions as a loading factor for Mediator at 
            ATF6alpha-regulated ER stress response genes.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Sep 28;118(39):e2108751118. doi: 
            10.1073/pnas.2108751118.
  PUBMED    34544872
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-09-28"; volume: "118(39):e2108751118. 
            doi"; pages: " 10.1073/pnas.2108751118"
COMMENT     Sequence Label: pGal4x5-E4T-G210
FEATURES             Location/Qualifiers
     source          1..1101
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        134..152
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     134..151
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    178..272
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     primer_bind     complement(549..568)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(550..568)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(649..666)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(900..919)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccatgggagg gcagtttcga gagagtgata gggtctgctt cagtaagcca gatgctacac
       61 aattaggctt gtacatattg tcgttagaac gcggctacaa ttaatacata accttatgta
      121 tcatacacat acgatttagg tgacactata gaatacaagc ttgcatgcct gcaggtcctc
      181 ggaggacagt actccgctcg gaggacagta ctccgctcgg aggacagtac tccgctcgga
      241 ggacagtact ccgctcggag gacagtactc cgactctaga ggatccccag tcctatatat
      301 actcgctctg cacttggccc ttttttacac ccataccctt cctccatcta taccacccta
      361 ctctcctttc ctcattattc ctcctattat cttctcctct tctctccttc ttctatattt
      421 cccaaatcta tcatcattca ctctcatccc ctcttccttc actcccattc tattctactc
      481 ctttcccttt ccatatcccc tccacccccc ttcctcccct ctttcaatct tatcccgaat
      541 tccggtctcc ctatagtgag tcgtattaat ttcgataagc cagctgcatt aatgaatcgg
      601 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
      661 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      721 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      781 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
      841 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      901 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      961 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtgcgctttc tcatagctca
     1021 cgctgtagta tctcagttcg tgtagtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1081 ccgttcagcc cgaccgctgc g
//
