LOCUS       sequence-331822-        8864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses cell cycle-dependent OsTIR1 fused with mEmerald and 
            Geminin (1-110). AKA ROLECCS G2(allows integration into AAVS1 
            safe-harbor).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Capece M, Tessari A, Mills J, Vinciguerra GLR, Louke D, Lin C, 
            McElwain BK, Miles WO, Coppola V, Davies AE, Palmieri D, Croce CM
  TITLE     A novel auxin-inducible degron system for rapid, cell cycle-specific
            targeted proteolysis.
  JOURNAL   Cell Death Differ. 2023 Aug 3. doi: 10.1038/s41418-023-01191-4.
  PUBMED    37537305
REFERENCE   2  (bases 1 to 8864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Differ. 2023 Aug 3. doi: 10.1038/s41418-023-01191-4."
COMMENT     Sequence Label: pDP1.2
FEATURES             Location/Qualifiers
     source          1..8864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    295..1098
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     enhancer        1155..1458
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1459..1662
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1613..1633
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1700..3424
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             join(3452..3454,3455..3457,3458..4168)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3497..3518)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3758..3777)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4105..4126
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4205..4534
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     polyA_signal    4711..4832
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4748..4767)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4802..4821
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        4841..5341
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5260..5278
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5363..5962
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5363..5382)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5859..5879
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    5969..6193
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    6196..7032
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(7066..7085)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7067..7085)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7092..7109)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7092..7108)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(join(7111..7902,7903..7971))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7734..7753
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8095..8683
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8584..8603
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8837..8854
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
      241 gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgc cctttgcttt
      301 ctctgaccag cattctctcc cctgggcctg tgccgctttc tgtctgcagc ttgtggcctg
      361 ggtcacctct acggctggcc cagatccttc cctgccgcct ccttcaggtt ccgtcttcct
      421 ccactccctc ttccccttgc tctctgctgt gttgctgccc aaggatgctc tttccggagc
      481 acttccttct cggcgctgca ccacgtgatg tcctctgagc ggatcctccc cgtgtctggg
      541 tcctctccgg gcatctctcc tccctcaccc aaccccatgc cgtcttcact cgctgggttc
      601 ccttttcctt ctccttctgg ggcctgtgcc atctctcgtt tcttaggatg gccttctccg
      661 acggatgtct cccttgcgtc ccgcctcccc ttcttgtagg cctgcatcat caccgttttt
      721 ctggacaacc ccaaagtacc ccgtctccct ggctttagcc acctctccat cctcttgctt
      781 tctttgcctg gacaccccgt tctcctgtgg attcgggtca cctctcactc ctttcatttg
      841 ggcagctccc ctacccccct tacctctcta gtctgtgcta gctcttccag ccccctgtca
      901 tggcatcttc caggggtccg agagctcagc tagtcttctt cctccaaccc gggcccctat
      961 gtccacttca ggacagcatg tttgctgcct ccagggatcc tgtgtccccg agctgggacc
     1021 accttatatt cccagggccg gttaatgtgg ctctggttct gggtactttt atctgtcccc
     1081 tccaccccac agtggggcaa ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     1141 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     1201 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     1261 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     1321 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     1381 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     1441 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     1501 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     1561 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     1621 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag
     1681 atccgctagc gctgccacca tgacatactt tcctgaagag gtcgtcgaac acatttttag
     1741 cttcctgcct gcacagagag atagaaacac agtgagcctg gtctgcaaag tgtggtacga
     1801 gatcgaacgc ctgagccgga gaggagtgtt cgtcggcaac tgctatgctg tgagagcagg
     1861 cagggtcgcc gctaggtttc caaatgtgcg cgcactgacc gtcaagggga aaccccactt
     1921 cgccgacttt aacctggtgc cccctgattg gggaggatac gccggccctt ggatcgaggc
     1981 agccgctcgc ggctgtcatg gactggagga actgcgcatg aagcgaatgg tggtctctga
     2041 cgaaagtctg gagctgctgg ctcggagctt ccctaggttt cgcgcactgg tgctgatttc
     2101 ttgcgaaggc ttcagcaccg atggactggc agccgtggcc tcccactgta agctgctgcg
     2161 ggagctggac ctccaggaga atgaagtgga ggatagaggc cccagatggc tgtcttgctt
     2221 cccagactca tgtaccagcc tggtgtccct gaactttgcc tgcatcaaag gcgaagtgaa
     2281 tgctgggtcc ctggagcggc tggtctcaag aagccccaac ctgaggtctc tgcggctgaa
     2341 ccggagcgtg agcgtggaca ctctggctaa gattctgctg agaaccccta acctggagga
     2401 tctgggaacc ggcaatctga cagacgattt ccagacagaa tcctacttta aactgacttc
     2461 tgccctggag aagtgtaaaa tgctgaggag tctgtcagga ttctgggatg cttcacccgt
     2521 gtgcctgagc tttatctacc ctctgtgtgc acagctgaca ggcctgaacc tgagctatgc
     2581 accaaccctg gacgccagtg atctgacaaa gatgatctca cgctgcgtga aactccagcg
     2641 actgtgggtg ctggactgta tttccgataa ggggctccag gtggtcgcca gctcctgcaa
     2701 ggacctccag gagctgagag tgttcccatc tgatttttac gtggccggat atagtgctgt
     2761 cactgaggaa ggcctggtgg cagtctcact gggatgccca aagctgaaca gcctgctgta
     2821 tttctgtcat cagatgacta atgctgcact ggtgaccgtc gccaagaact gccctaattt
     2881 cacccgattt cggctgtgta ttctggaacc aggcaaaccc gacgtggtca catcccagcc
     2941 actggatgaa gggtttggag ctatcgtgag agagtgcaag ggactccaga ggctgagcat
     3001 ttccggcctg ctgacagaca aagtgttcat gtacatcggc aagtatgcta agcagctgga
     3061 gatgctgagc attgcatttg ccggagactc cgataagggc atgatgcacg tgatgaacgg
     3121 gtgtaagaat ctgcgaaaac tggaaatccg ggacagccct ttcggggatg ccgctctgct
     3181 gggaaacttt gccagatacg agacaatgag gagcctgtgg atgtctagtt gcaatgtgac
     3241 tctgaagggc tgtcaggtcc tggctagtaa aatgcctatg ctgaacgtgg aagtcattaa
     3301 tgagcgggac gggtctaacg aaatggagga aaatcatggc gacctgccaa aggtggagaa
     3361 actgtatgtg tatcggacca ccgcaggggc aagagatgat gctcccaact ttgtgaagat
     3421 tctgggaggt tctggtggtt ctggtggttc catggtgagc aagggcgagg agctgttcac
     3481 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     3541 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     3601 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttgacct acggcgtgca
     3661 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     3721 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     3781 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     3841 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     3901 ggtctatatc accgccgaca agcagaagaa cggcatcaag gtgaacttca agacccgcca
     3961 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     4021 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa
     4081 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     4141 cactctcggc atggacgagc tgtacaagcc taggggatat ccatcacact ggcggccgct
     4201 cgagatgaat cccagtatga agcagaaaca agaagaaatc aaagagaata taaagaatag
     4261 ttctgtccca agaagaactc tgaagatgat tcagccttct gcatctggat ctcttgttgg
     4321 aagagaaaat gagctgtccg caggcttgtc caaaaggaaa catcggaatg accacttaac
     4381 atctacaact tccagccctg gggttattgt cccagaatct agtgaaaata aaaatcttgg
     4441 aggagtcacc caggagtcat ttgatcttat gattaaagaa aatccatcct ctcagtattg
     4501 gaaggaagtg gcagaaaaac ggagaaaggc gctgtgagga tccatcggat cccgggcccg
     4561 tcgacggtac cgcgggcccg ggatccaccg gggccgcgac tctagatcat aatcagccat
     4621 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     4681 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     4741 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     4801 tgtggtttgt ccaaactcat caatgtatct taagttgggg ttgcgccttt tccaaggcag
     4861 ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc
     4921 cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc
     4981 tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt
     5041 gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc
     5101 agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag
     5161 cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg
     5221 gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc
     5281 tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca
     5341 gggggatcca ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     5401 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     5461 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     5521 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     5581 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     5641 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     5701 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     5761 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     5821 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     5881 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     5941 gacccgcaag cccggtgcct gaggtaccct gtgccttcta gttgccagcc atctgttgtt
     6001 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     6061 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     6121 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     6181 gtgggctcta tggttactag ggacaggatt ggtgacagaa aagccccatc cttaggcctc
     6241 ctccttccta gtctcctgat attgggtcta acccccacct cctgttaggc agattcctta
     6301 tctggtgaca cacccccatt tcctggagcc atctctctcc ttgccagaac ctctaaggtt
     6361 tgcttacgat ggagccagag aggatcctgg gagggagagc ttggcagggg gtgggaggga
     6421 agggggggat gcgtgacctg cccggttctc agtggccacc ctgcgctacc ctctcccaga
     6481 acctgagctg ctctgacgcg gctgtctggt gcgtttcact gatcctggtg ctgcagcttc
     6541 cttacacttc ccaagaggag aagcagtttg gaaaaacaaa atcagaataa gttggtcctg
     6601 agttctaact ttggctcttc acctttctag tccccaattt atattgttcc tccgtgcgtc
     6661 agttttacct gtgagataag gccagtagcc agccccgtcc tggcagggct gtggtgagga
     6721 ggggggtgtc cgtgtggaaa actccctttg tgagaatggt gcgtcctagg tgttcaccag
     6781 gtcgtggccg cctctactcc ctttctcttt ctccatcctt ctttccttaa agagtcccca
     6841 gtgctatctg ggacatattc ctccgcccag agcagggtcc cgcttcccta aggccctgct
     6901 ctgggcttct gggtttgagt ccttggcaag cccaggagag gcgctcaggc ttccctgtcc
     6961 cccttcctcg tccaccatct catgcccctg gctctcctgc cccttcccta caggggttcc
     7021 tggctctgct ctaagggcga attcgcggcc gctaaattca attcgcccta tagtgagtcg
     7081 tattacaatt cactggccgt cgttttacaa ttaccaatgc ttaatcagtg aggcacctat
     7141 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7201 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7261 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7321 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7381 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7441 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7501 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7561 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7621 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7681 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     7741 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7801 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     7861 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     7921 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7981 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgaccaaaa tcccttaacg
     8041 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8101 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8161 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8221 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     8281 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     8341 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8401 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8461 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8521 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8581 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     8641 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8701 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     8761 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     8821 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag
//
