LOCUS       sequence-33183-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   van Kessel JC, Hatfull GF
  TITLE     Recombineering in Mycobacterium tuberculosis.
  JOURNAL   Nat Methods. 2007 Feb . 4(2):147-52.
  PUBMED    17179933
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2007 Feb . 4(2):147-52."
COMMENT     Sequence Label: pJV52
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     terminator      complement(204..250)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atcgcggcct cgagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac
       61 tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt
      121 aacatcagag attttgagac acaacgtggc tttccccccc ccccgacgtc aggtggctag
      181 ctgatcaccg cggccatgat ggcataaaac gaaaggccca gtctttcgac tgagcctttc
      241 gttttatttg atgcctggca gtcgatcgta cgctagttgc agatcaacca ccgcgtcgga
      301 attcgccggg gcgctcatgc gttgggcccg tcgaacatgt cgatgacagc cttcgcctcg
      361 tcgaacgtga ggtcggcggc gcgcgtggct ttgacgccgg ccgaatcggc gaggaacttg
      421 aaccaatcgg cgtcgttgta cttttcggcc ttctggatct cgccgaggcg cttcagctga
      481 tcgggattgg ccatctgcac gtcgggcgcc ggcgcggtgg cgcgctgcgc gcgttgtgcg
      541 cgcgacgact tcggtggcgt atcgacctcg tgcggctcgg cgatctcgcc gtcgatgtag
      601 tcggccgtcg gcttgacggt cgacggcagc gcgagcgcct gcgactgtga caggtcggtg
      661 cccgggcgat cgtcggcgcg gatcgccgcg tcgagccgcg tcgtcttcgg tgccagcttc
      721 agcacct
//
