LOCUS       sequence-33185-e         553 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 553)
  AUTHORS   van Kessel JC, Hatfull GF
  TITLE     Recombineering in Mycobacterium tuberculosis.
  JOURNAL   Nat Methods. 2007 Feb . 4(2):147-52.
  PUBMED    17179933
REFERENCE   2  (bases 1 to 553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2007 Feb . 4(2):147-52."
COMMENT     Sequence Label: pJV63
FEATURES             Location/Qualifiers
     source          1..553
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     terminator      complement(205..251)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta
       61 ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg
      121 taacatcaga gattttgaga cacaacgtgg ctttcccccc cccccgacgt caggtggcta
      181 gctgatcacc gcggccatga tggcataaaa cgaaaggccc agtctttcga ctgagccttt
      241 cgttttattt gatgcctggc agtcgatcgt acgctagttg cagatcaacc accgcgtcgg
      301 aattcgccgg ggcgctcatg cgttgggccc gtcgaacatg tcgatgacag ccttcgcctc
      361 gtcgaacgtg aggtcggcgg cgcgcgtggc tttgacgccg gccgaatcgg cgaggaactt
      421 gaaccaatcg gcgtcgttgt acttttcggc cttctggatc tcgccgaggc gcttcagctg
      481 atcgggattg gccatctgca cgtcnggcgc cggcgcggtg gcgcgctgcg cgcgttgtgc
      541 gcgcgacgac ttc
//
