LOCUS       sequence-331850-       17514 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Final destination vector for single Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17514)
  AUTHORS   Ursache R, Fujita S, Denervaud Tendon V, Geldner N
  TITLE     Combined fluorescent seed selection and multiplex CRISPR/Cas9 
            assembly for fast generation of multiple Arabidopsis mutants.
  JOURNAL   Plant Methods. 2021 Oct 30;17(1):111. doi: 
            10.1186/s13007-021-00811-9.
  PUBMED    34717688
REFERENCE   2  (bases 1 to 17514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2021-10-30"; volume: "17(1):111. doi"; pages: " 
            10.1186/s13007-021-00811-9"
COMMENT     Sequence Label: pRU61
FEATURES             Location/Qualifiers
     source          1..17514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(650..670)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(1671..1687)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1671..1687)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1684..1706)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1695..1711
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1719..1725,1726..1743,1744..1749))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1764..1785
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    1962..1986
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2507..3298
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3544..4132
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4033..4052
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4286..4303
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4318..4458
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4384..4403)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4544..4566
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4802..4996
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5062..6135)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6564..7193)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8514..8538
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8719..8741
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8733..8750
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8734..8750
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      8760..9011
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(9010..9705)
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     protein_bind    11959..12083
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(12124..12429)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(12226..12245)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(12749..13429)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     13344..13363
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(13483..13489,13490..13507,13508..13513))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(13538..13662)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(14174..14221)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(join(14222..17514,1..625))
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
ORIGIN
        1 gggtgaagat gtggcagttc tccttgaact tcgggaagtt gtcttgcacg atcctatgcg
       61 ggatggcggt gctaatgtcc tcggcgctga acacgttttt gcggttctcg taaaagccgc
      121 taaagtatgt cgtaaacttg tcgaacgacc ggaggagggc gttctcatgc tctgtggtcg
      181 tgacggtgcc cagttgcttc agcactttgc cattgaacag ctcggcctta aacagcccct
      241 tatagatttc ggcgtggcgc ttgttgatgg cgtcggtgag attatctgtg cgcccgatga
      301 agtagtcgtg gatagcgttg cgatacgtag cctgttcttc gatgagcgcg ttgcgtgtct
      361 cctccgtctt ctccttgcgg taggagtcga tggcggccga gaggttctcc cagtcgagct
      421 gcacgagctg caggcactgg tccgcgtagg tcttgtagat ccggtcgatg atcggcttga
      481 gctccttgta gtggtcgttc cgcgctttgt cctcctcgat aaagccctgc tcctggatgt
      541 gcttgagggt cttgccctgc ggaatcagct cgaaccgcag cgtcttgctg acctggtaga
      601 ggttggtgaa cccctcgaac tgcgtagccg caggcacccc gtgaatacca accttccgct
      661 tcttcttagg ggcgcgccga attcgctgca catacataac atatcaagat cagaacacac
      721 atatacacac acaaatacaa tcaagtcaac aactccaaaa agtccagatc tacatatata
      781 catacgtaaa taacaaaatc atgtaaataa tcacaatcat gtaatccaga tctatgcaca
      841 tatatatata cacaattaat aaaaaaaatg atataacaga tctatatcta tgtatgtaac
      901 aacacaatca gatgagagaa gtgatgtttt cagatctgta tacatacaaa cacaaacaga
      961 tgaacaattg atacgtagat ccatatgtat acgtacaatt agctacacga ttaaatgaaa
     1021 aaaatcaacg atttcggatt ggtacacaca aacgcaacaa tatgaagaaa ttcatatctg
     1081 attagatata aacataacca cgtgtagata cacagtcaaa tcaacaaatt tatagcttct
     1141 aaacggatga gatgaacaag ataaagatat tcacataagg catacataag ataagcagat
     1201 taacaaacta gcaataatac atacctaatt aaaacaagga ataacagaga gagagagaga
     1261 gagagagatt taccttgaaa atgaagagga gaagagagga tttcttaaaa ttgggggtag
     1321 agaaagaaag atgatgaatt gtgagaaagg agagatagaa gggggggttg tatatatagg
     1381 ctgtagaaga ttatttttgt gtttgaggcg gtgaaggaag aggggatctg actatgacac
     1441 gtttgcggtt acgtatttcg ataggagtct ttcaacgctt aacgccgtta ctctatatga
     1501 ccgtttgggc cgtaacgggg ccgtttgtta acgctgatgt tgattctttt ctttctttct
     1561 ttcttccttt tttaaagaag caattgtaca atcgttgcta gctgtcaaac ggataattcg
     1621 gatacggata tgcctatatt catatccgta atttttgaat tcgtaatcat gtcatagctg
     1681 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     1741 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     1801 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     1861 gcggggagag gcggtttgcg tattggctag agcaattcgg cgttaattca gtacattaaa
     1921 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc
     1981 ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat
     2041 acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact
     2101 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac
     2161 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg
     2221 atcaattcgg gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     2281 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     2341 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
     2401 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     2461 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg gggaagcggt
     2521 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     2581 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     2641 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     2701 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     2761 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     2821 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     2881 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     2941 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     3001 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     3061 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     3121 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     3181 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     3241 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg
     3301 tctagctaga aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta
     3361 gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat
     3421 ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgca tgaccaaaat
     3481 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3541 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3601 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3661 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     3721 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3781 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3841 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3901 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3961 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4021 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4081 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4141 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4201 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     4261 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     4321 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     4381 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     4441 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     4501 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     4561 cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg
     4621 gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc
     4681 agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga
     4741 gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag
     4801 ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga
     4861 aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta
     4921 cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat
     4981 gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg
     5041 ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg
     5101 gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg
     5161 caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc
     5221 gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc
     5281 gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg
     5341 gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc
     5401 tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct
     5461 aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg
     5521 ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg
     5581 ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac
     5641 gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga
     5701 aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga
     5761 aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg
     5821 cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc
     5881 atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga
     5941 cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg
     6001 ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc
     6061 cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg
     6121 tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg
     6181 cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc
     6241 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt
     6301 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag
     6361 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc
     6421 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt
     6481 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct
     6541 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc
     6601 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg
     6661 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac
     6721 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg
     6781 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc
     6841 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg
     6901 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg
     6961 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca
     7021 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg
     7081 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg
     7141 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc
     7201 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc
     7261 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca
     7321 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc
     7381 gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct
     7441 cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg
     7501 ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc
     7561 acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc
     7621 gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc
     7681 ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg
     7741 gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat
     7801 tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg
     7861 cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat
     7921 gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac
     7981 tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg
     8041 cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc
     8101 ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc
     8161 ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat
     8221 gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt
     8281 aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc
     8341 gactgcacta tagcagcgga ggggttggat caaagtactt taaagtactt taaagtactt
     8401 tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     8461 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     8521 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     8581 tccaagctca agctcattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     8641 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     8701 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctta
     8761 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
     8821 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     8881 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     8941 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
     9001 gttactagat cacttgtgcc ccagtttgct agggaggtcg cagtatctgg ccacagccac
     9061 ctcgtgctgc tcgacgtagg tctctttgtc ggcctccttg attctttcca gtctgtggtc
     9121 cacatagtag acgccgggca tcttgaggtt cttagcgggt ttcttggatc tgtatgtggt
     9181 cttgaagttg cagatcaggt ggcccccgcc cacgagcttc agggccatgt cgcttctgcc
     9241 ttccaggccg ccgtcagcgg ggtacagcat ctcggtgttg gcctcccagc cgagtgtttt
     9301 cttctgcatc acagggccgt tggatgggaa gttcacccct ctgatcttga cgttgtagat
     9361 gaggcagccg tcctggaggc tggtgtcctg ggtagcggtc agcacgcccc cgtcttcgta
     9421 tgtggtgact ctctcccatg tgaagccctc agggaaggac tgcttaaaga agtcggggat
     9481 gccctgggtg tggttgatga aggttctgct gccgtacatg aagctggtag ccaggatgtc
     9541 gaaggcgaag gggagagggc cgccctcgac caccttgatt ctcatggtct gggtgccctc
     9601 gtagggcttg ccttcgccct cggatgtgca cttgaagtgg tggttgttca cggtgccctc
     9661 catgtacagc ttcatgtgca tgttctcctt aatcagctcg ctcatagtag tgtgctggcc
     9721 accacgagta cggtcacggt catgttcccc accagtatgt tgctgtccgt agtactgagc
     9781 tctgtctttc agatcctgag ctttgcttcc caacttcatc cttgcactgt ccaacttgtc
     9841 tgatccctgt gggtgctctc ccgttgcgta cctattcaca atcaataggt atcataccaa
     9901 gcattttacc tatatagatg atatacacag acacattcaa gctagctagg tcaaatttcg
     9961 tatttagtta accaaatgtt ccctagaatt tgcaatttct tattgttaca tttgttattc
    10021 gaccaaaaaa attgatccaa agctactggc tcaacacatg catgcacata ttgcacaaaa
    10081 ttatgaagta agatgataaa tgtgtgctta cttgtaaatc caagagaaaa cggttatagc
    10141 ggcaatgcca aaccctccag aggaaagaaa accggtgatg aggagtgcaa ctgtgatgag
    10201 agccgggaca aggattgggc tgaagataac gagcagaggt gttgcaacag tcaaagctat
    10261 gacagttcca acaagggtaa ggctggagag aacaaggagg gaaccaccag ctgtgacagc
    10321 agttgcagct ttagcaatct gcctagactt ggagtagtca gatcctcgtc cggacatctg
    10381 gtactggtct cggtctcggc ccatcatcgg gtactggtct ctgccgatga tatcgtgatg
    10441 ggttcctcta gctgtatccg ccattttttt gttcttgttt actagagaga atgagatttt
    10501 gaatgagaga ttgatcaaaa agatgtgtat atattttgga agaggtagga gtgtttgtta
    10561 gatatattaa gaaaggagac gagaagtcac gtgtcaatgg agacatcatg caattgtagg
    10621 ccacgtgtta atggcgtcac catgcagccg ctgcatgtgt taacgagagg agtctattca
    10681 aagacacgtt tgttttttgc ctagctcaga atgtggcgtt ggcatcgaac cgaaacataa
    10741 aacgcaaaac gaacctttgg gctcgtcagt atgggtttgg gccttcaata gattccttct
    10801 atacggccca catatccact attggcaaca aaattagaaa aatttaggat ttagttttaa
    10861 aatttgttcc gcgacattct agaacaatga agaaaatgga tacaaataat aaatctatct
    10921 gcctcttaaa agttccaaat ttcaccacgt cactgctttg ataatgcagg tcctcgtcgt
    10981 ctctcctccg ctcatcgccg ccgcatctct ctctaaacct ctcaattctc tctctaaagc
    11041 cgcactctct ttctccagag ccaaacccat ttgtcctttc cctcaaacct ctcgacgacc
    11101 aatctccgta tacaaatccc caatgaacaa ccttttcaac agactcggat tcgggtctcg
    11161 tcctcaagcc caagctgacc catcttccgc cgcaatcgcc caaggacccg acgatgatgt
    11221 tccgtcttca ggtcagcaat tcgcgcaatt tggcgccggt tgtttctggg gagtggagct
    11281 agcttaccag agagttcctg gtgtgaccaa gaccgaggtt gggtatagcc atggcatcgt
    11341 gcacaatccg agttacgagg atgtctgtac tggcactacg ggacataacg aggttgttag
    11401 agttcagtat gatcccaaag agtgtagctt tgagagcttg cttgatgttt tctggaatcg
    11461 tcatgatcca accaccttga atcgtcaggt gattgattgc tttaacccta aaaatctaga
    11521 cgttatcagt atcagatcgt aatttgaatc aatagagagc tctatgtgtg ttctgcattt
    11581 ggggctgaat tgtttattag ataacaacga ctagattcac ctgtttagac gaactctctg
    11641 tcccttagtc tttagttcta catacatttg gtagtctcaa gtaactattg gctaagttgt
    11701 tatgagctag ttagaatctc aggccttgtt gatatgagga ttcgtgtttg attatgtgga
    11761 agtgacattg ccatttccat tttcgtatgc gatcttgtac ttgagtctgt tcttgttcat
    11821 gttactatac ctacatacac ttgaagaagc ttgcatgcct gcaggtcgac tctagaggat
    11881 ccactagtcg gtccggacgt cttaattaac catggacgcg tcctcaggcc cgggcctagg
    11941 ctggatcgga attatcgaac cactttgtac aagaaagctg aacgagaaac gtaaaatgat
    12001 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa
    12061 acacaacata tccagtcact atggtcgacc tgcagactgg ctgtgtataa gggagcctga
    12121 catttatatt ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct
    12181 gagatcagcc acttcttccc cgataacgga gaccggcaca ctggccatat cggtggtcat
    12241 catgcgccag ctttcatccc cgatatgcac caccgggtaa agttcacggg ggactttatc
    12301 tgacagcaga cgtgcactgg ccagggggat caccatccgt cgcccgggcg tgtcaataat
    12361 atcactctgt acatccacaa acagacgata acggctctct cttttatagg tgtaaacctt
    12421 aaactgcatt tcaccagccc ctgttctcgt cagcaaaaga gccgttcatt tcaataaacc
    12481 gggcgacctc agccatccct tcctgatttt ccgctttcca gcgttcggca cgcagacgac
    12541 gggcttcatt ctgcatggtt gtgcttacca gaccggagat attgacatca tatatgcctt
    12601 gagcaactga tagctgtcgc tgtcaactgt cactgtaata cgctgcttca tagcatacct
    12661 ctttttgaca tacttcgggt atacatatca gtatatattc ttataccgca aaaatcagcg
    12721 cgcaaatacg catactgtta tctggctttt agtaagccgg atcctctaga ttacgccccg
    12781 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
    12841 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
    12901 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
    12961 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
    13021 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
    13081 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
    13141 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
    13201 ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
    13261 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
    13321 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
    13381 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
    13441 tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac gagccggaag
    13501 cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt
    13561 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
    13621 atcattttac gtttctcgtt cagctttttt gtacaaactt gttagggata acaggcctag
    13681 ggagctcaag cctatactgt acttaacttg attgcataat tacttgatca tagactcata
    13741 gtaaacttga ttacacagat aagtgaagaa acaaaccaat tcaagacata accaaagaga
    13801 ggtgaaagac tgttttatat gtctaacatt gcaccttaat atcacactgt tagttccttt
    13861 cttacttaaa ttcaacccat taaagtaaaa acaacagata ataataattt gagaatgaac
    13921 aaaaggacca tatcatttat taactcttat ccatccattt gcattttgat gtccgaaaac
    13981 aaaaactgaa agaacacagt aaattacaag cagaacaaat gatagaagaa aacagctttt
    14041 ccaatgccat aatactcaaa cttagtagga ttctggtgtg tgggcaatga aactgatgca
    14101 ttgaacttga cgaacgttgt cgaaaccgat gatacggacg aaagctggga ggcctggagc
    14161 tcggcgcgcc tcacttcttt ttcttagcct gtccggcctt tttggtggca gcaggacgct
    14221 tattgcggag ctcttggata taggcaagcc aatcctggtt gctgatgccg ttctgcagct
    14281 taaggtcctt gctctccttg agatggttca acaacagttg tcctttcaat gctatgtggt
    14341 atgcgccgtt agcatcggca tccatcggcc actcaggatt ctgaaatcta gaatcgaagc
    14401 acacgccatt gagatcacgc acaggggagt tgatgtaatc ctccccagtg gcagcatttg
    14461 aatttctcat ttgcagcacg gatctgatga gcgctaccat cgtgtcaata gcgtggctat
    14521 catcgttctc gagcagtttg gggaggatat ttgacccgtc gcggaaaacg atgcctttct
    14581 cctccaggag ggcgatgagt tcgtttgcgg ggtacaggtc acggtaacgc ccggtgaatc
    14641 tatggttctc aattacaggg acaatcctct tgcccgcgat gaaaggcgtg cctttggcgt
    14701 cgaattgggt ttcatttttc tcaaaaacga tatcccaagc gggcatgaag ccagggagtc
    14761 cccgctggaa ggagaggttc cgattcatct tgaaatgcag gatgaagtct ccggttttta
    14821 catcatagtg caggaagtcg aaaccctcca ggaaatgctt acgcgactca tggttcttga
    14881 tggtcttcca gacaaacggg tccacgaatc cggtcaaggg atcaatcttt gaggtatatg
    14941 gggccgggac atagaaaagg aagccgctct gggtgcccat cttcgcaaat gacgtaaact
    15001 ggtcggtcag ctgatacgga ttgaggaccc ccccgacctt ctccgccgga tagtccttga
    15061 gcaccaggca attgagcttg tcgataagca ttttctcgaa ctgttggtac accgccttct
    15121 cggcgatgcc tgtgcgcttg gatttgaatc cgaaattgag attctcgagg accacgacgg
    15181 cttggtagtg gatcatgaga tccactatct catggatgac ctggctgaga tagccctgct
    15241 tcaggtcttt gatcgtgccc actacggacc aggcctgcct cgcggcgacg cgttccttct
    15301 cccggttgtc gagcttcttc tggtagtcga actgctggat ggtattgagc gaccgttgct
    15361 cgagaatctt tccggtggag tcgatgacgg tgatgtagat gaggttccgc tccccccggt
    15421 caatcccgat tatgggtgtc tctgggtgct ccttgagata cgcgttgacc cgctggttga
    15481 acttggacgg gctattcgcg gcctggtagt tcagcgtgat ggggacatgg aaaaagaact
    15541 tgtcagaggt aaaccgccga tcctttatga tctcgtggct gacctccttc gtgatgacgt
    15601 ttggcaggag cgcgcgggcc tcgtcgctga ggtcgtgtga gaggcggtgg ttcacgtaat
    15661 cgtacagctc ctgatacagt gtatcaggga ttggcgtctt ttgatctttc agcttcttgt
    15721 tcagcatctt ctcaccgagg cgatgagcca tgcgcttcat cctggacttc ggcctgtaga
    15781 agagttctgc ctggccgttc agctttatgc tcgtcttcgc gagattttcc gggctgaaga
    15841 ggcctgtcca gtagagggtg tgcaggtttg gcttcccgtg gtggcccttc gcgaagtcct
    15901 tgttatagat ttggaaaaga tacaacttgc cggtttcgac ggcgtccatg atctcctttt
    15961 ccgcgatacg ctgaaacgag atgtgataca ggagtgggtt gagttcggcg tagtactccc
    16021 cgaggtcctt gtactgggaa gaggggcgga gggaagagag atcgatggag gttgtcttgg
    16081 tgtacttgct gaggaaatcg cgcgtgaagt cgatccactt gcagagcgcc tctctatacc
    16141 ccttttgatc gcccgtcttc ttggcgtagg cggtctggaa cttcttgggt tccttttctg
    16201 gattattcag atcgtatatc tccttggtga tctcgagcgg ctcgatgaag ttgttggaga
    16261 ggaggatggg ggtggtatgg gtctgaaagt gcgccgtcac cgccttaagc tgagtgctgc
    16321 acttcggtat catctttgca gcgtcaggaa aataatcgta gtacatcttg tcgaagccct
    16381 cggaggtctt ctcagttggc tcgaagctga gcgccttgta cctgcctttt tgtttcggca
    16441 taattcccaa ataatacaac ccatttttca cgaaaagaat ggctccgttg ttcttctctt
    16501 tgttaacgtc ccacccgctg gccagggtgg gcatttggaa gttcagcttg aacttctcca
    16561 cggaatacgg cttcttggtc gcgtaattgc gcgccttatt atagaagctc aagctaggtt
    16621 ccatctcaag cttgatcccg gtgaggcggg cgctaaactc cggatcgacc tcgttggact
    16681 cgtccacggc gaaccagtcg agaaggtggt acagccccag gaggctgtcc agctgggact
    16741 tgaggatttc cttttcctct tgctttttca gggtcgtcgg cagcggctgg tccagggccg
    16801 cgtgggcgtg agagaggatc tccgaggtct tctgcttgaa agcctcggac agctccttgc
    16861 ccgcggcgga gatgatctcc tgcaggttaa tgtcctcgtg cttgaggctc ctttgcactt
    16921 tctctttcgc cgacttcgtg atcttgccgg tgagctcgct gatgcgccgc tcgtagaggg
    16981 cgttgcggag ggtgtcccag tggtcgcaca gggcgctgga gatggtttcc agcttcttat
    17041 gggagatgaa gatgtgggtg agatcgatgc tattgagctc attgaacagc gcctcggcgg
    17101 tttcgagcac attctcgttg cggaggaggg tcttgtactt gcagaaggac tggatgacct
    17161 cctcgtcgct cttgaactcc tcgaggatga aggacaacgt gttgcggtcg gagaggatct
    17221 gcttaaacag ggggataaac ctatgtggga gtgaggcaat gatgtgcgcg gtttcgtcgt
    17281 tcttctggat ggcgagattg agcacctcgt tcaggccctt gatcttctcg gtgccggcct
    17341 cgcgggagat gccgccgagg agctggttgt agaggtcgat ctgggtctgc gtcagcagct
    17401 gattgtagaa ggggaaggag aacacctctt cgatcgaggt ggacacgaag atcccgatgg
    17461 ccttcttcac gttctcgaag tgctcgcgga gggacggcac ggccgtgatg aggc
//
