LOCUS       sequence-331863-        4920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Replaces Addgene plasmid 118459. Yeast tagging vector to create a 
            gene fusion with yeast optimized mVenus with CaUra3 selection; 
            Contains the linker region at the 5' end of the FP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pFA6a-link-ymVenus-URA3
FEATURES             Location/Qualifiers
     source          1..4920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..30)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(184..772)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(264..283)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(943..1734,1735..1803))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1566..1585
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1804..1908)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1976..1994
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2032..2054)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2154..2173
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        2254..2272
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2254..2271
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             2342..3058
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      3099..3286
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3154..3178)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        3361..3704
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      4523..4720
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(4827..4846)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4828..4846)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
       61 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      121 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      181 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      241 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      301 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      361 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      421 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      481 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
      541 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
      601 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
      661 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
      721 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
      781 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
      841 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
      901 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      961 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     1021 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     1081 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     1141 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     1201 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     1261 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     1321 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     1381 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     1441 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     1501 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     1561 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     1621 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     1681 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     1741 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     1801 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1861 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     1921 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     1981 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     2041 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     2101 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     2161 agagcagatt gtactgagag tgcaccatat ggacatattg tcgttagaac gcggctacaa
     2221 ttaatacata accttatgta tcatacacat acgatttagg tgacactata gaacgcggcc
     2281 gccagctgaa gcttcgtacg ctgcaggtcg acggatcggt gacggtgctg gtttaattaa
     2341 catgtctaaa ggtgaagaat tattcactgg tgttgtccca attttggttg aattagatgg
     2401 tgatgttaat ggtcacaaat tttctgtctc cggtgaaggt gaaggtgatg ctacttacgg
     2461 taaattgacc ttaaaattga tttgtactac tggtaaattg ccagttccat ggccaacctt
     2521 agtcactact ttaggttatg gtttgcaatg ttttgctaga tacccagatc atatgaaaca
     2581 acatgacttt ttcaagtctg ccatgccaga aggttatgtt caagaaagaa ctattttttt
     2641 caaagatgac ggtaactaca agaccagagc tgaagtcaag tttgaaggtg ataccttagt
     2701 taatagaatc gaattaaaag gtattgattt taaagaagat ggtaacattt taggtcacaa
     2761 attggaatac aactataact ctcacaatgt ttacatcact gctgacaaac aaaagaatgg
     2821 tatcaaagct aacttcaaaa ttagacacaa cattgaagat ggtggtgttc aattagctga
     2881 ccattatcaa caaaatactc caattggtga tggtccagtc ttgttaccag acaaccatta
     2941 cttatcctat caatctaaat tatccaaaga tccaaacgaa aagagagacc acatggtctt
     3001 gttagaattt gttactgctg ctggtattac ccatggtatg gatgaattgt acaaataagc
     3061 ggccgcctcg aggtcgacgg cgcgccactt ctaaataagc gaatttctta tgatttatga
     3121 tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt
     3181 aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt
     3241 ctcaggtata gtatgaggtc gctcttattg accacacctc taccggcaga tccgctaggg
     3301 ataacagggt aatatagatc tgtttagctt gcctcgtccc cgccgggtca cccggccagc
     3361 gacatggagg cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga
     3421 tgtgactgtc gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata
     3481 cattttgatg gccgcacggc gcgaagcaaa aattacggct cctcgctgcg gacctgcgag
     3541 cagggaaacg ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga
     3601 aatataaaag gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc
     3661 ttgctaggat acagttctca catcacatcc gaacataaac aaccatgaca gtcaacacta
     3721 agacctatag tgagagagca gaaactcatg cctcaccagt agcacaacga ttatttcgat
     3781 taatggaact gaagaaaacc aatttatgtg catcaattga tgttgatacc actaaggaat
     3841 tccttgaatt aattgataaa ttgggtcctt atgtatgctt aatcaagact catattgata
     3901 taatcaatga tttttcctat gaatccacta ttgaaccatt attagaactt tcacgtaaac
     3961 atcaatttat gatttttgaa gatagaaaat ttgctgatat tggtaatacc gtgaagaaac
     4021 aatatattgg tggagtttat aaaattagta gttgggcaga tattactaat gctcatggtg
     4081 tcactgggaa tggagtagtt gaaggattaa aacagggagc taaagaaacc accaccaacc
     4141 aagagccaag agggttattg atgttagctg aattatcatc agtgggatca ttagcatatg
     4201 gagaatattc tcaaaaaact gttgaaattg ctaaatccga taaggaattt gttattggat
     4261 ttattgccca acgtgatatg ggtggacaag aagaaggatt tgattggctt attatgacac
     4321 ctggagttgg attagatgat aaaggtgatg gattaggaca acaatataga actgttgatg
     4381 aagttgttag cactggaact gatattatca ttgttggtag aggattgttt ggtaaaggaa
     4441 gagatccaga tattgaaggt aaaaggtata gagatgctgg ttggaatgct tatttgaaaa
     4501 agactggcca attataatca gtactgacaa taaaaagatt cttgttttca agaacttgtc
     4561 atttgtatag tttttttata ttgtagttgt tctattttaa tcaaatgtta gcgtgattta
     4621 tatttttttt cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat
     4681 atcatgcgtc aatcgtatgt gaatgctggt cgctatactg ctgtcgattc gatactaacg
     4741 ccgccatcca gtgtcgaaaa cgagctcgaa ttcatcgatg atatcagatc cactagtggc
     4801 ctatgcggcc gcggatctgc cggtctccct atagtgagtc gtattaattt cgataagcca
     4861 ggttaacctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
//
