LOCUS       sequence-331868-        8459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces equimolar expression of mGreenLantern and mCherry for use 
            in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8459)
  AUTHORS   Botman D, de Groot DH, Wehrens M, van Rossum K, Yska S, van der 
            Kieft B, Albert V, Minghetti N, Goedhart J, Teusink B
  TITLE     Side-by-side systematic characterization of novel FPs in budding 
            yeast
  JOURNAL   bioRxiv 2025.06.27.661975; doi: 
            https://doi.org/10.1101/2025.06.27.661975
REFERENCE   2  (bases 1 to 8459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.27.661975; doi: https://doi.org/10.1101/2025.06.27.661975"
COMMENT     Sequence Label: mGreenLantern-T2A-mCherry pDRF1-GW
FEATURES             Location/Qualifiers
     source          1..8459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             221..274
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      290..299
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             296..1006
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(441..459)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     709..728
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(1016..1040)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(1102..1121)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1103..1121)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1269..1456
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1324..1348)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(1468..1485)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1468..1484)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1477..1499)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1920..3266
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3377..4180)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(4181..4396)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(4421..4439)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4507..4611
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4612..4680,4681..5472)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4830..4849)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5643..6231
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6132..6151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6385..6402
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6519..6540
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6555..6560,6561..6578,6579..6585)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6593..6609
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6598..6620
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6617..6633
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6617..6633
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(6663..7118)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6800..6821)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7012..7031
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7926..7950
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(7957..7959,7960..7962,7963..8459,1..214)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(8002..8023)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ccgccacaac gttgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc
       61 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagccatc agtccaaact
      121 gagcaaagac cccaacgaga agcgcgatca catggtcctg aaggagaggg tgaccgccgc
      181 cgggattaca catgacatgg acgagctgta caagtccgga gagggcagag gaagtcttct
      241 aacatgcggt gacgtggagg agaatcccgg ccctggggat ccaccggtcg ccaccatggt
      301 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
      361 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
      421 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
      481 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
      541 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
      601 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
      661 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
      721 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
      781 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
      841 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
      901 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
      961 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaaagcg gccgcaccca
     1021 gctttcttgt acaaagtggt gtagttaatt catgatggcc gctgcaggtc gacctcgagg
     1081 gggggcccgg tacccaattc gccctatagt gagtcgtatt acgcgcggat ccagctttgg
     1141 acttcttcgc cagaggtttg gtcaagtctc caatcaaggt tgtcggcttg tctaccttgc
     1201 cagaaattta cgaaaagatg gaaaagggtc aaatcgttgg tagatacgtt gttgacactt
     1261 ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa
     1321 taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga
     1381 gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc gctcttattg
     1441 accacacctc taccggcatg ccaattcact ggccgtcgtt ttacaacgtc gtgactggga
     1501 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     1561 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     1621 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataat
     1681 cggatcgtac ttgttaccca tcattgaatt ttgaacatcc gaacctggga gttttccctg
     1741 aaacagatag tatatttgaa cctgtataat aatatatagt ctagcgcttt acggaagaca
     1801 atgtatgtat ttcggttcct ggagaaacta ttgcatctat tgcataggta atcttgcacg
     1861 tcgcatcccc ggttcatttt ctgcgtttcc atcttgcact tcaatagcat atctttgtta
     1921 acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc
     1981 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaaa gcgctatttt
     2041 accaacgaag aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat
     2101 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgagagcgct
     2161 attttaccaa caaagaatct atacttcttt tttgttctac aaaaatgcat cccgagagcg
     2221 ctatttttct aacaaagcat cttagattac tttttttctc ctttgtgcgc tctataatgc
     2281 agtctcttga taactttttg cactgtaggt ccgttaaggt tagaagaagg ctactttggt
     2341 gtctattttc tcttccataa aaaaagcctg actccacttc ccgcgtttac tgattactag
     2401 cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc gattatattc tataccgatg
     2461 tggattgcgc atactttgtg aacagaaagt gatagcgttg atgattcttc attggtcaga
     2521 aaattatgaa cggtttcttc tattttgtct ctatatacta cgtataggaa atgtttacat
     2581 tttcgtattg ttttcgattc actctatgaa tagttcttac tacaattttt ttgtctaaag
     2641 agtaatacta gagataaaca taaaaaatgt agaggtcgag tttagatgca agttcaagga
     2701 gcgaaaggtg gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat
     2761 acttttgagc aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc gttacagtcc
     2821 ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct
     2881 ctgaagttcc tatactttct agctagagaa taggaacttc ggaataggaa cttcaaagcg
     2941 tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca gctcactgtt
     3001 cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa gaacggcata
     3061 gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga tgaaaggtag
     3121 tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct tccttcagca
     3181 ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc atccttcaat
     3241 gctatcattt cctttgatat tggatcgatc cgatgataag ctgtcaaaca tgagaattgg
     3301 gtaataactg atataattaa attgaagctc taatttgtga gtttagtata catgcattta
     3361 cttataatac agttttttag ttttgctggc cgcatcttct caaatatgct tcccagcctg
     3421 cttttctgta acgttcaccc tctaccttag catcccttcc ctttgcaaat agtcctcttc
     3481 caacaataat aatgtcagat cctgtagaga ccacatcatc cacggttcta tactgttgac
     3541 ccaatgcgtc tcccttgtca tctaaaccca caccgggtgt cataatcaac caatcgtaac
     3601 cttcatctct tccacccatg tctctttgag caataaagcc gataacaaaa tctttgtcgc
     3661 tcttcgcaat gtcaacagta cccttagtat attctccagt agatagggag cccttgcatg
     3721 acaattctgc taacatcaaa aggcctctag gttcctttgt tacttcttct gccgcctgct
     3781 tcaaaccgct aacaatacct gggcccacca caccgtgtgc attcgtaatg tctgcccatt
     3841 ctgctattct gtatacaccc gcagagtact gcaatttgac tgtattacca atgtcagcaa
     3901 attttctgtc ttcgaagagt aaaaaattgt acttggcgga taatgccttt agcggcttaa
     3961 ctgtgccctc catggaaaaa tcagtcaaga tatccacatg tgtttttagt aaacaaattt
     4021 tgggacctaa tgcttcaact aactccagta attccttggt ggtacgaaca tccaatgaag
     4081 cacacaagtt tgtttgcttt tcgtgcatga tattaaatag cttggcagca acaggactag
     4141 gatgagtagc agcacgttcc ttatatgtag ctttcgacat gatttatctt cgtttcctgc
     4201 atgtttttgt tctgtgcagt tgggttaaga atactgggca atttcatgtt tcttcaacac
     4261 tacatatgcg tatatatacc aatctaagtc tgtgctcctt ccttcgttct tccttctgtt
     4321 cggagattac cgaatcaaaa aaatttcaag gaaaccgaaa tcaaaaaaaa gaataaaaaa
     4381 aaaatgatga attgaaaagc taattcttga agacgaaagg gcctcgtgat acgcctattt
     4441 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     4501 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4561 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4621 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4681 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4741 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4801 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4861 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4921 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4981 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5041 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     5101 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     5161 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     5221 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     5281 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     5341 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5401 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5461 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5521 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5581 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5641 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5701 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5761 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5821 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5881 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5941 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6001 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6061 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     6361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     6421 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     6481 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     6541 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     6601 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct taccgcatca
     6661 ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     6721 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     6781 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     6841 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     6901 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     6961 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     7021 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     7081 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccaag cttcctgaaa
     7141 cggagaaaca taaacaggca ttgctgggat cacccataca tcactctgtt ttgcctgacc
     7201 ttttccggta atttgaaaac aaacccggtc tcgaagcgga gatccggcga taattaccgc
     7261 agaaataaac ccatacacga gacgtagaac cagccgcaca tggccggaga aactcctgcg
     7321 agaatttcgt aaactcgcgc gcattgcatc tgtatttcct aatgcggcac ttccaggcct
     7381 cgatcgagac cgtttatcca ttgctttttt gttgtctttt tccctcgttc acagaaagtc
     7441 tgaagaagct atagtagaac tatgagcttt ttttgtttct gttttccttt tttttttttt
     7501 tacctctgtg gaaattgtta ctctcacact ctttagttcg tttgtttgtt ttgtttattc
     7561 caattatgac cggtgacgaa acgtggtcga tggtgggtac cgcttatgct cccctccatt
     7621 agtttcgatt atataaaaag gccaaatatt gtattatttt caaatgtcct atcattatcg
     7681 tctaacatct aatttctctt aaattttttc tctttctttc ctataacacc aatagtgaaa
     7741 atcttttttt cttctatatc tacaaaaact ttttttttct atcaacctcg ttgataaatt
     7801 ttttctttaa caatcgttaa taattaatta attggaaaat aaccattttt tctctctttt
     7861 atacacacat tcaaaagaaa gaaaaaaaat ataccccagc ctcgactagt gaattcgcgg
     7921 ccatcacaag tttgtacaaa aaagcaggct gctagcatgg tgagcaaggg cgaggagctg
     7981 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     8041 agcgtccgcg gcgagggcga gggcgatgcc accaacggca agctgaccct gaagttcatc
     8101 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt aggctacggc
     8161 gtggcctgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     8221 atgcccgaag gctacgtcca ggagcgcacc atctctttca aggacgacgg tacctacaag
     8281 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcgt gctgaagggc
     8341 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa cttcaacagc
     8401 cacaaggtct atatcacggc cgacaagcag aagaacggca tcaaggctaa cttcaagac
//
