LOCUS       sequence-33187-e         472 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 472)
  AUTHORS   van Kessel JC, Hatfull GF
  TITLE     Efficient point mutagenesis in mycobacteria using single-stranded 
            DNA recombineering: characterization of antimycobacterial drug 
            targets.
  JOURNAL   Mol Microbiol. 2008 Mar . 67(5):1094-107.
  PUBMED    18221264
REFERENCE   2  (bases 1 to 472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Microbiol. 2008 Mar . 67(5):1094-107."
COMMENT     Sequence Label: pJV62
FEATURES             Location/Qualifiers
     source          1..472
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     terminator      complement(210..256)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atttaatcgc ggcctcgagc aagacgtttc ccgttgaata tggctcataa caccccttgt
       61 attactgntt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg
      121 caatgtaaca tcagagattt tgagacacaa cgtggctttc cccccccccc gacgtcaggt
      181 ggctagctga tcaccgcggc catgatggca taaaacgaaa ggcccagtct ttcgactgag
      241 cctttcgttt tatttgatgc ctggcagtcg atcgtacgct agttgcagat caaccaccgc
      301 gtcggaattc gccggggcgc tcatgcgttg ggcccgtcga acatgtcgat gacagccttc
      361 gcctcgtcga acgtgaggtc ggcggcgcgc gtggctttga cgccggccga atcggcgagg
      421 aacttgaacc aatcggcgtc gttgtacttt tcggccttct ggatctcgcc ga
//
