LOCUS       sequence-331873-        4914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Replaces Addgene Plasmid 125703. Yeast tagging vector to create a 
            gene fusion with yeast optimized mNeongreen with CaUra3 selection; 
            Contains the linker region at the 5' end of the FP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pFA6a-link-ymNeongreen-URA3
FEATURES             Location/Qualifiers
     source          1..4914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..23)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     123..142
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        223..241
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     223..240
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      566..575
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      1062..1249
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1117..1141)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        1324..1667
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      2486..2683
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2790..2809)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2791..2809)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2896..2913)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3067..3655)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3147..3166)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3826..4617,4618..4686))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4449..4468
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4687..4791)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4859..4877
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
       61 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
      121 tgcggcatca gagcagattg tactgagagt gcaccatatg gacatattgt cgttagaacg
      181 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactatag
      241 aacgcggccg ccagctgaag cttcgtacgc tgcaggtcga cggatcggtg acggtgctgg
      301 tttaattaac atggtctcta agggtgaaga agataacatg gcttctttgc cagccaccca
      361 cgagttgcac atcttcggtt ccattaacgg tgtcgacttc gatatggtcg gtcaaggtac
      421 cggtaaccca aacgacggtt acgaagaatt gaacttgaag tctaccaagg gcgacttgca
      481 gttctcccct tggattttgg ttccacacat cggttacggt ttccaccaat acttgccata
      541 cccagacggt atgtccccat tccaagccgc catggttgac ggttctggtt accaagtcca
      601 cagaaccatg caattcgaag acggtgcttc cttgaccgtg aactacagat acacctacga
      661 aggttctcac atcaagggcg aagcccaagt taagggtacc ggtttccctg ctgacggtcc
      721 agtcatgact aactccttga ccgctgctga ctggtgtcgt tccaagaaga cctacccaaa
      781 cgataagacc atcatttcca ctttcaagtg gtcctacacc accggtaacg gcaagagata
      841 ccgctctacc gctcgtacca cctacacctt cgccaagcca atggctgcca actacttgaa
      901 gaaccagcct atgtacgtct tccgtaagac cgagctgaag cattccaaga ctgaattgaa
      961 cttcaaggaa tggcaaaagg ctttcaccga cgtcatgggt atggacgaat tgtacaagta
     1021 agcggccgcc tcgaggtcga cggcgcgcca cttctaaata agcgaatttc ttatgattta
     1081 tgatttttat tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact
     1141 cttaggtttt aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc
     1201 tttctcaggt atagtatgag gtcgctctta ttgaccacac ctctaccggc agatccgcta
     1261 gggataacag ggtaatatag atctgtttag cttgcctcgt ccccgccggg tcacccggcc
     1321 agcgacatgg aggcccagaa taccctcctt gacagtcttg acgtgcgcag ctcaggggca
     1381 tgatgtgact gtcgcccgta catttagccc atacatcccc atgtataatc atttgcatcc
     1441 atacattttg atggccgcac ggcgcgaagc aaaaattacg gctcctcgct gcggacctgc
     1501 gagcagggaa acgctcccct cacagacgcg ttgaattgtc cccacgccgc gcccctgtag
     1561 agaaatataa aaggttagga tttgccactg aggttcttct ttcatatact tccttttaaa
     1621 atcttgctag gatacagttc tcacatcaca tccgaacata aacaaccatg acagtcaaca
     1681 ctaagaccta tagtgagaga gcagaaactc atgcctcacc agtagcacaa cgattatttc
     1741 gattaatgga actgaagaaa accaatttat gtgcatcaat tgatgttgat accactaagg
     1801 aattccttga attaattgat aaattgggtc cttatgtatg cttaatcaag actcatattg
     1861 atataatcaa tgatttttcc tatgaatcca ctattgaacc attattagaa ctttcacgta
     1921 aacatcaatt tatgattttt gaagatagaa aatttgctga tattggtaat accgtgaaga
     1981 aacaatatat tggtggagtt tataaaatta gtagttgggc agatattact aatgctcatg
     2041 gtgtcactgg gaatggagta gttgaaggat taaaacaggg agctaaagaa accaccacca
     2101 accaagagcc aagagggtta ttgatgttag ctgaattatc atcagtggga tcattagcat
     2161 atggagaata ttctcaaaaa actgttgaaa ttgctaaatc cgataaggaa tttgttattg
     2221 gatttattgc ccaacgtgat atgggtggac aagaagaagg atttgattgg cttattatga
     2281 cacctggagt tggattagat gataaaggtg atggattagg acaacaatat agaactgttg
     2341 atgaagttgt tagcactgga actgatatta tcattgttgg tagaggattg tttggtaaag
     2401 gaagagatcc agatattgaa ggtaaaaggt atagagatgc tggttggaat gcttatttga
     2461 aaaagactgg ccaattataa tcagtactga caataaaaag attcttgttt tcaagaactt
     2521 gtcatttgta tagttttttt atattgtagt tgttctattt taatcaaatg ttagcgtgat
     2581 ttatattttt tttcgcctcg acatcatctg cccagatgcg aagttaagtg cgcagaaagt
     2641 aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata ctgctgtcga ttcgatacta
     2701 acgccgccat ccagtgtcga aaacgagctc gaattcatcg atgatatcag atccactagt
     2761 ggcctatgcg gccgcggatc tgccggtctc cctatagtga gtcgtattaa tttcgataag
     2821 ccaggttaac ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     2881 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2941 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3001 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3061 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3121 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3181 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3241 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3301 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3361 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3421 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     3481 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     3541 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3601 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3661 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3721 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3781 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     3841 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     3901 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     3961 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     4021 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     4081 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     4141 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     4201 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     4261 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     4321 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     4381 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     4441 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     4501 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     4561 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     4621 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     4681 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     4741 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     4801 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     4861 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaa
//
