LOCUS       sequence-331878-        9280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LexA activated transgene expression of ultrafast protein calcium 
            sensor under the hsp70 promoter in D. melanogaster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Zhang Y, Rozsa M, Bushey D, Zheng J, Reep D, Broussard GJ, Tsang A, 
            Tsegaye G, Patel R, Narayan S, Li JX, Zhang R, Ahrens MB, Turner GC,
            Wang SS-H, Svoboda K, Korff W, Schreiter ER, Hasseman JP, Kolb I, 
            Looger LL
  TITLE     jGCaMP8 Fast Genetically Encoded Calcium Indicators
  JOURNAL   Janelia Research Campus, Online Resource (2020) 
            doi:10.25378/janelia.13148243.
REFERENCE   2  (bases 1 to 9280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Janelia 
            Research Campus, Online Resource (2020) 
            doi:10.25378/janelia.13148243."
COMMENT     Sequence Label: pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8m-p10
FEATURES             Location/Qualifiers
     source          1..9280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..29)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1907..1976
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2201..2219)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2671..2742,3144..3417,3492..4146,4208..4523,4727..4858,
                     4929..5543)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     promoter        6955..7193
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     7140..7161
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          7208..7274
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             7307..7324
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
       61 tcttagactc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttaccgtcga cgatgtaggt cacggtctcg aagccgcggt
     1921 gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta ctccacctca cccatctggt
     1981 ccatcatgat gaacgggtcg aggtggcggt agttgatccc ggcgaacgcg cggcgcaccg
     2041 ggaagccctc gccctcgaaa ccgctgggcg cggtggtcac ggtgagcacg ggacgtgcga
     2101 cggcgtcggc gggtgcggat acgcggggca gcgtcagcgg gttctcgacg gtcacggcgg
     2161 gcatgtcgac aagccgaaca tatgggcgcg cctagtatgt atgtaagtta ataaaaccca
     2221 tttttgcgga aagtagataa aaaaaacatt tttttttttt actgcactgg atatcattga
     2281 acttatctga tcagttttaa atttacttcg atccaagggt atttgatgta ccaggttctt
     2341 tcgattacct ctcactcaaa atgacattcc actcaaagtc agcgctgttt gcctccttct
     2401 ctgtccacag aaatatcgcc gtctctttcg ccgctgcgtc cgctatctct ttcgccaccg
     2461 tttgtagcgt tacgtagcgt caatgtccgc cttcagttgc attttgtcag cggtttcgtg
     2521 acgaagctcc aagcggttta cgccatcaat taaacacaaa gtgctgtgcc aaaactcctc
     2581 tcgcttctta tttttgtttg ttttttgagt gattggggtg gtgattggtt ttgggtgggt
     2641 aagcagggga aagtgtgaaa aatcccggca atgggccaag aggatcagga gctattaatt
     2701 cgcggaggca gcaaacaccc atctgccgag catctgaaca atgtgagtag tacatgtgca
     2761 tacatcttaa gttcacttga tctataggaa ctgcgattgc aacatcaaat tgtctgcggc
     2821 gtgagaactg cgacccacaa aaatcccaaa ccgcaattgc acaaacaaat agtgacacga
     2881 aacagattat tctggtagct gttctcgcta tataagacaa tttttgagat catatcatga
     2941 tcaagacatc taaaggcatt cattttcgac tatattcttt tttacaaaaa atataacaac
     3001 cagatatttt aagctgatcc tagatgcaca aaaaataaat aaaagtataa acctacttcg
     3061 taggatactt cggggtactt tttgttcggg gttagatgag cataacgctt gtagttgata
     3121 tttgagatcc cctatcattg cagggtgaca gcggagcggc ttcgcagagc tgcattaacc
     3181 agggcttcgg gcaggccaaa aactacggca cgctccggcc acccagtccg ccggaggact
     3241 ccggttcagg gagcggccaa ctagccgaga acctcaccta tgcctggcac aatatggaca
     3301 tctttggggc ggtcaatcag ccgggctccg gatggcggca gctggtcaac cggacacgcg
     3361 gactattctg caacgagcga cacataccgg cgcccaggaa acatttgctc aagaacggtg
     3421 agtttctatt cgcagtcggc tgatctgtgt gaaatcttaa taaagggtcc aattaccaat
     3481 ttgaaactca gtttgcggcg tggcctatcc gggcgaactt ttggccgtga tgggcagttc
     3541 cggtgccgga aagacgaccc tgctgaatgc ccttgccttt cgatcgccgc agggcatcca
     3601 agtatcgcca tccgggatgc gactgctcaa tggccaacct gtggacgcca aggagatgca
     3661 ggccaggtgc gcctatgtcc agcaggatga cctctttatc ggctccctaa cggccaggga
     3721 acacctgatt ttccaagcca tggtgcggat gccacgacat ctgacctatc ggcagcgagt
     3781 ggcccgcgtg gatcaggtga tccaggagct ttcgctcagc aaatgtcagc acacgatcat
     3841 cggtgtgccc ggcagggtga aaggtctgtc cggcggagaa aggaagcgtc tggcattcgc
     3901 ctccgaggct ctaaccgatc cgccgcttct gatctgcgat gagcccacct ccggactgga
     3961 ctcctttacc gcccacagcg tcgtccaggt gctgaagaag ctgtcgcaga agggcaagac
     4021 cgtcatcctg accattcatc agccgtcttc cgagctgttt gagctctttg acaagatcct
     4081 tctgatggcc gagggcaggg tagctttctt gggcactccc agcgaagccg tcgacttctt
     4141 ttcctagtga gttcgatgtg tttattaagg gtatctagta ttacataaca tctcaactcc
     4201 tatccagcgt gggtgcccag tgtcctacca actacaatcc ggcggacttt tacgtacagg
     4261 tgttggccgt tgtgcccgga cgggagatcg agtcccgtga tcggatcgcc aagatatgcg
     4321 acaattttgc cattagcaaa gtagcccggg atatggagca gttgttggcc accaaaaatc
     4381 tggagaagcc actggagcag ccggagaatg ggtacaccta caaggccacc tggttcatgc
     4441 agttccgggc ggtcctgtgg cgatcctggc tgtcggtgct caaggaacca ctcctcgtaa
     4501 aagtgcgact tattcagaca acggtgagtg gttccagtgg aaacaaatga tataacgctt
     4561 acaattcttg gaaacaaatt cgctagattt tagatagaat tgcctgattc cacacccttc
     4621 ttagtttttt tcaatgagat gtatagttta tagttttgca gaagataaat aaatttcatt
     4681 taactcgcga atattaatga gatgcgagta acattttaat ttgcagatgg ttgccatctt
     4741 gattggcctc atctttttgg gccaacaact cacgcaagtg ggtgtgatga atatcaacgg
     4801 agccatcttc ctcttcctga ccaacatgac ctttcaaaac gtctttgcca cgataaatgt
     4861 aagtcatgtt tagaatacat ttgcatttca ataatttact aactttctaa tgaatcgatt
     4921 cgatttaggt gttcacctca gagctgccag tttttatgag ggaggcccga agtcgacttt
     4981 atcgctgtga cacatacttt ctgggcaaaa cgattgccga attgccgctt tttctcacag
     5041 tgccactggt cttcacggcg attgcctatc cgatgatcgg actgcgggcc ggagtgctgc
     5101 acttcttcaa ctgcctggcg ctggtcactc tggtggccaa tgtgtcaacg tccttcggat
     5161 atctaatatc ctgcgccagc tcctcgacct cgatggcgct gtctgtgggt ccgccggtta
     5221 tcataccatt cctgctcttt ggcggcttct tcttgaactc gggctcggtg ccagtatacc
     5281 tcaaatggtt gtcgtacctc tcatggttcc gttacgccaa cgagggtctg ctgattaacc
     5341 aatgggcgga cgtggagccg ggcgaaatta gctgcacatc gtcgaacacc acgtgcccca
     5401 gttcgggcaa ggtcatcctg gagacgctta acttctccgc cgccgatctg ccgctggact
     5461 acgtgggtct ggccattctc atcgtgagct tccgggtgct cgcatatctg gctctaagac
     5521 ttcgggcccg acgcaaggag tagccgacat atatccgaaa taactgcttg tttttttttt
     5581 ttaccattat taccatcgtg tttactgttt attgccccct caaaaagcta atgtaattat
     5641 atttgtgcca ataaaaacaa gatatgacct atagaataca agtatttccc cttcgaacat
     5701 ccccacaagt agactttgga tttgtcttct aaccaaaaga cttacacacc tgcatacctt
     5761 acatcaaaaa ctcgtttatc gctacataaa acaccgggat atatttttta tatacatact
     5821 tttcaaatcg cgcgccctct tcataattca cctccaccac accacgtttc gtagttgctc
     5881 tttcgctgtc tcccacccgc tctccgcaac acattcacct tttgttcgac gaccttggag
     5941 cgactgtcgt tagttccgcg cgattcggtt cgctcaaatg gttccgagtg gttcatttcg
     6001 tctcaataga aattagtaat aaatatttgt atgtacaatt tatttgctcc aatatatttg
     6061 tatatatttc cctcacagct atatttattc taatttaata ttatgacttt ttaaggtaat
     6121 tttttgtgac ctgttcggag tgattagcgt tacaatttga actgaaagtg acatccagtg
     6181 tttgttcctt gtgtagatgc atctcaaaaa aatggtgggc ataatagtgt tgtttatata
     6241 tatcaaaaat aacaactata ataataagaa tacatttaat ttagaaaatg cttggatttc
     6301 actggaacta gggcgcgcct ccggaacata atggtgcagg gcgctgactt ccgcgtttcc
     6361 agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt
     6421 ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta
     6481 aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt
     6541 ggccagggcc gcaagcttgc atgcctgcag gttactgtac atccatacag taagtactgt
     6601 acatccatac agtaagtact gtacatccat acagtaagta ctgtacatcc atacagtaag
     6661 tactgtacat ccatacagta agcggagact ctagccctag ggcatgcctg caggttactg
     6721 tacatccata cagtaagtac tgtacatcca tacagtaagt actgtacatc catacagtaa
     6781 gcggagactc tagcgctagc gcatgcctgc aggttactgt acatccatac agtaagtact
     6841 gtacatccat acagtaagta ctgtacatcc atacagtaag tactgtacat ccatacagta
     6901 agtactgtac atccatacag taagcggaga ctctagcact agtgacgtcg agcgccggag
     6961 tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc aaagtgaaca
     7021 cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta aacaatctgc
     7081 agtaaagtgc aagttaaagt gaatcaatta aaagtaacca gcaaccaagt aaatcaactg
     7141 caactactga aatctgccaa gaagtaatta ttgaatacaa gaagagaact ctgaatagat
     7201 ctaaaaggta ggttcaacca ctgatgccta ggcacaccga aacgactaac cctaattctt
     7261 atcctttact tcaggcggcc gcaacttaaa aaaaaaaatc aaaatgcacc accaccatca
     7321 tcataccagg cggaaaaaga ccttcaaaga ggtcgccaca gccgtgaaaa tcatcggccg
     7381 attgatggga ttgaagatta acgtgtatat taaggccgat aaacagaaga acggcatcaa
     7441 ggccaatttc catatacggc acaacatcga agacggaggc gtccagctgg cctaccatta
     7501 ccagcagaac acacctatcg gcgatggacc ggtgctgttg ccggataatc actacctgag
     7561 tgtggaaagt aagttgtcga aggaccctaa cgaaaaacgc gaccacatgg tgttgctcga
     7621 attcgtgacg gcggccggaa tcacactggg tatggacgaa ctgtacaagg gcggaaccgg
     7681 aggatcgatg gtaagcaaag gcgaggagct gtttaccggc gtggtgccga ttttggtgga
     7741 gttggacggc gatgtcaacg gccacaagtt cagcgtgagc ggtgagggtg agggcgatgc
     7801 aacctacggc aaactgacat tgaaattcat ctgcaccacg ggaaaactcc cggttccatg
     7861 gccaacactg gtcaccactc tgacttatgg cgttcagtgc tttagccggt accccgacca
     7921 tatgaaacag catgacttct ttaagtccgc gatgcccgag ggttacatcc aagagcgaac
     7981 aatcttcttc aaggatgacg gcaattacaa gacgcgcgca gaagtcaagt tcgagggcga
     8041 taccctggtg aacaggattg aattgaaagg cattgacttt aaggaggatg gaaacatcct
     8101 gggccataag ctggagtaca acctgccgga ccagctgaca gaggagcaaa tcgcggagta
     8161 caaggaagcc tttagcctgt tcgacaagga cggtgatggc acgattacca caaaagagtt
     8221 gggcaccgtc atgcgcagtc tgggacaaaa tcctactgaa gctgagctgc aggacatgat
     8281 taacgaggtc gatgcagatg gcgacggcac tatcgacttt cccgagttct tgacaatgat
     8341 ggcccgcaag atgaagtacc gggataccga ggaggagatc cgcgaagcct ttggcgtgtt
     8401 cgataaagac ggcaatggct acattagcgc tgcggagctg cgccatgtca tgaccaactt
     8461 gggcgagaag ctcacggacg aggaagtgga cgaaatgata cgcgaagcgg acatcgatgg
     8521 cgatggccag gtcaactatg aggaattcgt tcaaatgatg accgcaaagt aaggtgtaca
     8581 aatctagaat gaatcgtttt taaaataaca aatcaattgt tttataatat tcgtacgatt
     8641 ctttgattat gtaataaaat gtgatcatta ggaagattac gaaaaatata aaaaatatga
     8701 gttctgtgtg tataacaaat gctgtaaacg ccacaattgt gtttgttgca aataaaccca
     8761 tgattatttg attaaaattg ttgttttctt tgttcataga caatagtgtg ttttgcctaa
     8821 acgtgtactg cataaactcc atgcgagtgt atagcgagct agtggctaac gcttgcccca
     8881 ccaaagtaga ttcgtcaaaa tcctcaattt catcaccctc ctccaagttt aacatttggc
     8941 cgtcggaatt aacttctaaa gatgccacat aatctaataa atgaaataga gattcaaacg
     9001 tggcgtcatc gtccgtttcg accatttccg aaaagaactc gggcataaac tctatgattt
     9061 ctctggacgt ggtgttgtcg aaactctcaa agtacgcagt caggaacgtg cgcgacatgt
     9121 cgtcgggaaa ctcgcgcgga aacatgttgt tgtaaccgaa cgggtcccat agcgccaaaa
     9181 ccaaatctgc cagcgtcaat agaatgagca cgatgccgac aatggagctg gcttggatag
     9241 cgattcgagt taacggccgg ccgtttaaac gaattcttga
//
