LOCUS       sequence-331879-        9280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LexA activated transgene expression of ultrafast protein calcium 
            sensor under the hsp70 promoter in D. melanogaster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Zhang Y, Rozsa M, Bushey D, Zheng J, Reep D, Broussard GJ, Tsang A, 
            Tsegaye G, Patel R, Narayan S, Li JX, Zhang R, Ahrens MB, Turner GC,
            Wang SS-H, Svoboda K, Korff W, Schreiter ER, Hasseman JP, Kolb I, 
            Looger LL
  TITLE     jGCaMP8 Fast Genetically Encoded Calcium Indicators
  JOURNAL   Janelia Research Campus, Online Resource (2020) 
            doi:10.25378/janelia.13148243.
REFERENCE   2  (bases 1 to 9280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Janelia 
            Research Campus, Online Resource (2020) 
            doi:10.25378/janelia.13148243."
COMMENT     Sequence Label: pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8f-p10
FEATURES             Location/Qualifiers
     source          1..9280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        33..137
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(138..206,207..998)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(356..375)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1169..1757
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1658..1677
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1844..1913
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2138..2156)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2608..2679,3081..3354,3429..4083,4145..4460,4664..4795,
                     4866..5480)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     promoter        6892..7130
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     7077..7098
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          7145..7211
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             7244..7261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9228..9246)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tagactcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
       61 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      121 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
      181 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
      241 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
      301 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
      361 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
      421 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
      481 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
      541 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
      601 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
      661 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
      721 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
      781 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
      841 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
      901 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
      961 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     1021 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     1081 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     1141 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1201 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1261 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     1321 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1381 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1441 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1501 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     1561 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     1621 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     1681 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     1741 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     1801 ggccttttgc tcacatgtta ccgtcgacga tgtaggtcac ggtctcgaag ccgcggtgcg
     1861 ggtgccaggg cgtgcccttg ggctccccgg gcgcgtactc cacctcaccc atctggtcca
     1921 tcatgatgaa cgggtcgagg tggcggtagt tgatcccggc gaacgcgcgg cgcaccggga
     1981 agccctcgcc ctcgaaaccg ctgggcgcgg tggtcacggt gagcacggga cgtgcgacgg
     2041 cgtcggcggg tgcggatacg cggggcagcg tcagcgggtt ctcgacggtc acggcgggca
     2101 tgtcgacaag ccgaacatat gggcgcgcct agtatgtatg taagttaata aaacccattt
     2161 ttgcggaaag tagataaaaa aaacattttt tttttttact gcactggata tcattgaact
     2221 tatctgatca gttttaaatt tacttcgatc caagggtatt tgatgtacca ggttctttcg
     2281 attacctctc actcaaaatg acattccact caaagtcagc gctgtttgcc tccttctctg
     2341 tccacagaaa tatcgccgtc tctttcgccg ctgcgtccgc tatctctttc gccaccgttt
     2401 gtagcgttac gtagcgtcaa tgtccgcctt cagttgcatt ttgtcagcgg tttcgtgacg
     2461 aagctccaag cggtttacgc catcaattaa acacaaagtg ctgtgccaaa actcctctcg
     2521 cttcttattt ttgtttgttt tttgagtgat tggggtggtg attggttttg ggtgggtaag
     2581 caggggaaag tgtgaaaaat cccggcaatg ggccaagagg atcaggagct attaattcgc
     2641 ggaggcagca aacacccatc tgccgagcat ctgaacaatg tgagtagtac atgtgcatac
     2701 atcttaagtt cacttgatct ataggaactg cgattgcaac atcaaattgt ctgcggcgtg
     2761 agaactgcga cccacaaaaa tcccaaaccg caattgcaca aacaaatagt gacacgaaac
     2821 agattattct ggtagctgtt ctcgctatat aagacaattt ttgagatcat atcatgatca
     2881 agacatctaa aggcattcat tttcgactat attctttttt acaaaaaata taacaaccag
     2941 atattttaag ctgatcctag atgcacaaaa aataaataaa agtataaacc tacttcgtag
     3001 gatacttcgg ggtacttttt gttcggggtt agatgagcat aacgcttgta gttgatattt
     3061 gagatcccct atcattgcag ggtgacagcg gagcggcttc gcagagctgc attaaccagg
     3121 gcttcgggca ggccaaaaac tacggcacgc tccggccacc cagtccgccg gaggactccg
     3181 gttcagggag cggccaacta gccgagaacc tcacctatgc ctggcacaat atggacatct
     3241 ttggggcggt caatcagccg ggctccggat ggcggcagct ggtcaaccgg acacgcggac
     3301 tattctgcaa cgagcgacac ataccggcgc ccaggaaaca tttgctcaag aacggtgagt
     3361 ttctattcgc agtcggctga tctgtgtgaa atcttaataa agggtccaat taccaatttg
     3421 aaactcagtt tgcggcgtgg cctatccggg cgaacttttg gccgtgatgg gcagttccgg
     3481 tgccggaaag acgaccctgc tgaatgccct tgcctttcga tcgccgcagg gcatccaagt
     3541 atcgccatcc gggatgcgac tgctcaatgg ccaacctgtg gacgccaagg agatgcaggc
     3601 caggtgcgcc tatgtccagc aggatgacct ctttatcggc tccctaacgg ccagggaaca
     3661 cctgattttc caagccatgg tgcggatgcc acgacatctg acctatcggc agcgagtggc
     3721 ccgcgtggat caggtgatcc aggagctttc gctcagcaaa tgtcagcaca cgatcatcgg
     3781 tgtgcccggc agggtgaaag gtctgtccgg cggagaaagg aagcgtctgg cattcgcctc
     3841 cgaggctcta accgatccgc cgcttctgat ctgcgatgag cccacctccg gactggactc
     3901 ctttaccgcc cacagcgtcg tccaggtgct gaagaagctg tcgcagaagg gcaagaccgt
     3961 catcctgacc attcatcagc cgtcttccga gctgtttgag ctctttgaca agatccttct
     4021 gatggccgag ggcagggtag ctttcttggg cactcccagc gaagccgtcg acttcttttc
     4081 ctagtgagtt cgatgtgttt attaagggta tctagtatta cataacatct caactcctat
     4141 ccagcgtggg tgcccagtgt cctaccaact acaatccggc ggacttttac gtacaggtgt
     4201 tggccgttgt gcccggacgg gagatcgagt cccgtgatcg gatcgccaag atatgcgaca
     4261 attttgccat tagcaaagta gcccgggata tggagcagtt gttggccacc aaaaatctgg
     4321 agaagccact ggagcagccg gagaatgggt acacctacaa ggccacctgg ttcatgcagt
     4381 tccgggcggt cctgtggcga tcctggctgt cggtgctcaa ggaaccactc ctcgtaaaag
     4441 tgcgacttat tcagacaacg gtgagtggtt ccagtggaaa caaatgatat aacgcttaca
     4501 attcttggaa acaaattcgc tagattttag atagaattgc ctgattccac acccttctta
     4561 gtttttttca atgagatgta tagtttatag ttttgcagaa gataaataaa tttcatttaa
     4621 ctcgcgaata ttaatgagat gcgagtaaca ttttaatttg cagatggttg ccatcttgat
     4681 tggcctcatc tttttgggcc aacaactcac gcaagtgggt gtgatgaata tcaacggagc
     4741 catcttcctc ttcctgacca acatgacctt tcaaaacgtc tttgccacga taaatgtaag
     4801 tcatgtttag aatacatttg catttcaata atttactaac tttctaatga atcgattcga
     4861 tttaggtgtt cacctcagag ctgccagttt ttatgaggga ggcccgaagt cgactttatc
     4921 gctgtgacac atactttctg ggcaaaacga ttgccgaatt gccgcttttt ctcacagtgc
     4981 cactggtctt cacggcgatt gcctatccga tgatcggact gcgggccgga gtgctgcact
     5041 tcttcaactg cctggcgctg gtcactctgg tggccaatgt gtcaacgtcc ttcggatatc
     5101 taatatcctg cgccagctcc tcgacctcga tggcgctgtc tgtgggtccg ccggttatca
     5161 taccattcct gctctttggc ggcttcttct tgaactcggg ctcggtgcca gtatacctca
     5221 aatggttgtc gtacctctca tggttccgtt acgccaacga gggtctgctg attaaccaat
     5281 gggcggacgt ggagccgggc gaaattagct gcacatcgtc gaacaccacg tgccccagtt
     5341 cgggcaaggt catcctggag acgcttaact tctccgccgc cgatctgccg ctggactacg
     5401 tgggtctggc cattctcatc gtgagcttcc gggtgctcgc atatctggct ctaagacttc
     5461 gggcccgacg caaggagtag ccgacatata tccgaaataa ctgcttgttt ttttttttta
     5521 ccattattac catcgtgttt actgtttatt gccccctcaa aaagctaatg taattatatt
     5581 tgtgccaata aaaacaagat atgacctata gaatacaagt atttcccctt cgaacatccc
     5641 cacaagtaga ctttggattt gtcttctaac caaaagactt acacacctgc ataccttaca
     5701 tcaaaaactc gtttatcgct acataaaaca ccgggatata ttttttatat acatactttt
     5761 caaatcgcgc gccctcttca taattcacct ccaccacacc acgtttcgta gttgctcttt
     5821 cgctgtctcc cacccgctct ccgcaacaca ttcacctttt gttcgacgac cttggagcga
     5881 ctgtcgttag ttccgcgcga ttcggttcgc tcaaatggtt ccgagtggtt catttcgtct
     5941 caatagaaat tagtaataaa tatttgtatg tacaatttat ttgctccaat atatttgtat
     6001 atatttccct cacagctata tttattctaa tttaatatta tgacttttta aggtaatttt
     6061 ttgtgacctg ttcggagtga ttagcgttac aatttgaact gaaagtgaca tccagtgttt
     6121 gttccttgtg tagatgcatc tcaaaaaaat ggtgggcata atagtgttgt ttatatatat
     6181 caaaaataac aactataata ataagaatac atttaattta gaaaatgctt ggatttcact
     6241 ggaactaggg cgcgcctccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga
     6301 ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg
     6361 cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg
     6421 caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc
     6481 cagggccgca agcttgcatg cctgcaggtt actgtacatc catacagtaa gtactgtaca
     6541 tccatacagt aagtactgta catccataca gtaagtactg tacatccata cagtaagtac
     6601 tgtacatcca tacagtaagc ggagactcta gccctagggc atgcctgcag gttactgtac
     6661 atccatacag taagtactgt acatccatac agtaagtact gtacatccat acagtaagcg
     6721 gagactctag cgctagcgca tgcctgcagg ttactgtaca tccatacagt aagtactgta
     6781 catccataca gtaagtactg tacatccata cagtaagtac tgtacatcca tacagtaagt
     6841 actgtacatc catacagtaa gcggagactc tagcactagt gacgtcgagc gccggagtat
     6901 aaatagaggc gcttcgtcta cggagcgaca attcaattca aacaagcaaa gtgaacacgt
     6961 cgctaagcga aagctaagca aataaacaag cgcagctgaa caagctaaac aatctgcagt
     7021 aaagtgcaag ttaaagtgaa tcaattaaaa gtaaccagca accaagtaaa tcaactgcaa
     7081 ctactgaaat ctgccaagaa gtaattattg aatacaagaa gagaactctg aatagatcta
     7141 aaaggtaggt tcaaccactg atgcctaggc acaccgaaac gactaaccct aattcttatc
     7201 ctttacttca ggcggccgca acttaaaaaa aaaaatcaaa atgcaccacc accatcatca
     7261 taccaggcgg aaaaagacct tcaaagaggt cgccacagcc gtgaaaatca tcgcccgatt
     7321 gatgggattg aagattaacg tgtatattaa ggccgataaa cagaagaacg gcatcaaggc
     7381 caatttccat atacggcaca acatcgaaga cggaggcgtc cagctggcct accattacca
     7441 gcagaacaca cctatcggcg atggaccggt gctgttgccg gataatcact acctgagtgt
     7501 ggaaagtaag ttgtcgaagg accctaacga aaaacgcgac cacatggtgt tgctcgaatt
     7561 cgtgacggcg gccggaatca cactgggtat ggacgaactg tacaagggcg gaaccggagg
     7621 atcgatggta agcaaaggcg aggagctgtt taccggcgtg gtgccgattt tggtggagtt
     7681 ggacggcgat gtcaacggcc acaagttcag cgtgagcggt gagggtgagg gcgatgcaac
     7741 ctacggcaaa ctgacattga aattcatctg caccacggga aaactcccgg ttccatggcc
     7801 aacactggtc accactctga cttatggcgt tcagtgcttt agccggtacc ccgaccatat
     7861 gaaacagcat gacttcttta agtccgcgat gcccgagggt tacatccaag agcgaacaat
     7921 cttcttcaag gatgacggca attacaagac gcgcgcagaa gtcaagttcg agggcgatac
     7981 cctggtgaac aggattgaat tgaaaggcat tgactttaag gaggatggaa acatcctggg
     8041 ccataagctg gagtacaacc tgccggacca gctgacagag gagcaaatcg cggagttcaa
     8101 ggaagccttt agcctgttcg acaaggacgg tgatggcacg attaccacaa aagagttggg
     8161 caccgtcatg cgcagtctgg gactgaatcc tactgaagct gagctgcagg acatgattaa
     8221 cgaggtcgat gcagatggcg acggcactat cgactttccc gagttcttga caatgatggc
     8281 ccgcaagatg aagtaccggg ataccgagga ggagatccgc gaagcctttg gcgtgttcga
     8341 taaagacggc aatggctaca ttagcgctgc ggagctgcgc catgtcatga ccaacttggg
     8401 cgagaagctc acggacgagg aagtggacga aatgatacgc gaagcggaca tcgatggcga
     8461 tggccaggtc aactatgagg aattcgttca aatgatgacc gcaaagtaag gtgtacaaat
     8521 ctagaatgaa tcgtttttaa aataacaaat caattgtttt ataatattcg tacgattctt
     8581 tgattatgta ataaaatgtg atcattagga agattacgaa aaatataaaa aatatgagtt
     8641 ctgtgtgtat aacaaatgct gtaaacgcca caattgtgtt tgttgcaaat aaacccatga
     8701 ttatttgatt aaaattgttg ttttctttgt tcatagacaa tagtgtgttt tgcctaaacg
     8761 tgtactgcat aaactccatg cgagtgtata gcgagctagt ggctaacgct tgccccacca
     8821 aagtagattc gtcaaaatcc tcaatttcat caccctcctc caagtttaac atttggccgt
     8881 cggaattaac ttctaaagat gccacataat ctaataaatg aaatagagat tcaaacgtgg
     8941 cgtcatcgtc cgtttcgacc atttccgaaa agaactcggg cataaactct atgatttctc
     9001 tggacgtggt gttgtcgaaa ctctcaaagt acgcagtcag gaacgtgcgc gacatgtcgt
     9061 cgggaaactc gcgcggaaac atgttgttgt aaccgaacgg gtcccatagc gccaaaacca
     9121 aatctgccag cgtcaataga atgagcacga tgccgacaat ggagctggct tggatagcga
     9181 ttcgagttaa cggccggccg tttaaacgaa ttcttgaaga cgaaagggcc tcgtgatacg
     9241 cctattttta taggttaatg tcatgataat aatggtttct
//
